Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   A9404_RS05445 Genome accession   NZ_CP016027
Coordinates   1231712..1232950 (+) Length   412 a.a.
NCBI ID   WP_066099258.1    Uniprot ID   A0A191ZG85
Organism   Halothiobacillus diazotrophicus strain LS2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1226712..1237950
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9404_RS05430 (A9404_05430) - 1228364..1229386 (+) 1023 WP_066099255.1 MerR family transcriptional regulator -
  A9404_RS05435 (A9404_05435) - 1229460..1229918 (+) 459 WP_066099256.1 PAS domain-containing protein -
  A9404_RS05440 (A9404_05440) pilB 1230000..1231700 (+) 1701 WP_066099257.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A9404_RS05445 (A9404_05445) pilC 1231712..1232950 (+) 1239 WP_066099258.1 type II secretion system F family protein Machinery gene
  A9404_RS05450 (A9404_05450) pilD 1232988..1233878 (+) 891 WP_066099259.1 A24 family peptidase Machinery gene
  A9404_RS05455 (A9404_05455) coaE 1233922..1234545 (+) 624 WP_082922762.1 dephospho-CoA kinase -
  A9404_RS05460 (A9404_05460) - 1234561..1234992 (-) 432 WP_066099261.1 hypothetical protein -
  A9404_RS05465 (A9404_05465) - 1235080..1236120 (+) 1041 WP_066099262.1 folate-binding protein YgfZ -
  A9404_RS05470 (A9404_05470) - 1236264..1237055 (+) 792 WP_066099263.1 thiazole synthase -
  A9404_RS05475 (A9404_05475) trmB 1237088..1237813 (+) 726 WP_066099264.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 45230.74 Da        Isoelectric Point: 9.9517

>NTDB_id=185419 A9404_RS05445 WP_066099258.1 1231712..1232950(+) (pilC) [Halothiobacillus diazotrophicus strain LS2]
MAVATSAKKAKPDEKTIFVYDAKNKDGKLVKGEMAAHNEMVVRAEVRRLGLTVVRVKKKPKPLFSNSKGIVPRDIAVFAR
QLATMMTAGLPIVQALDIMAQSAEKVSMKELVTTVKNDVESGQTLANAMDKHRKYFDELFVNLVRAGEDSGSLELVLDRV
AVYKEKTESLKGKIKKALFYPIAVVVVAFIVTAILLIFVIPQFKQLFDGFGATLPAFTLFVIGLSETFQKYWYFIFGGIG
VAIFLFTYVKQRSDRFNHFLDRMILKMPIFGPLTEKAAIARFSRTLATMFSAGVPLVEAMPPTAGSTGNAVFKDAVMNMR
DIIAAGSPLKVGMQQSKLFPVMVVQMVAIGEETGALDTMLGKVADMYEEEVDNMVDAMSSLMEPMIMAFLGVVVGGLVIA
MYLPIFKLGSVV

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=185419 A9404_RS05445 WP_066099258.1 1231712..1232950(+) (pilC) [Halothiobacillus diazotrophicus strain LS2]
ATGGCAGTCGCGACCTCCGCAAAGAAAGCCAAACCGGACGAAAAGACGATTTTCGTCTATGACGCGAAGAACAAGGACGG
AAAACTCGTCAAGGGCGAAATGGCGGCACACAACGAGATGGTCGTCCGCGCCGAAGTGCGCCGCCTCGGGCTCACCGTCG
TGCGGGTCAAGAAAAAGCCCAAACCGCTCTTTTCGAACTCCAAAGGCATCGTCCCCCGCGATATCGCGGTCTTCGCCCGC
CAGCTCGCCACCATGATGACCGCGGGCCTGCCCATCGTTCAGGCGTTGGACATCATGGCGCAGAGTGCGGAAAAGGTATC
GATGAAGGAACTGGTCACCACGGTCAAGAATGATGTGGAAAGTGGCCAGACCCTGGCCAACGCCATGGACAAGCACCGCA
AGTATTTCGACGAACTGTTCGTCAACCTGGTCCGGGCTGGCGAAGATTCCGGCTCGCTGGAACTCGTGCTGGATCGGGTC
GCCGTCTATAAGGAAAAGACCGAAAGCCTGAAAGGCAAGATCAAAAAAGCCCTGTTTTACCCGATCGCCGTTGTGGTTGT
CGCCTTCATCGTGACTGCCATCCTGCTGATTTTCGTGATCCCGCAATTCAAACAGCTCTTTGACGGATTCGGTGCGACCC
TGCCGGCATTCACCCTGTTCGTGATCGGCTTGTCCGAAACATTCCAGAAGTACTGGTATTTCATTTTCGGTGGCATCGGG
GTGGCCATTTTCCTGTTTACCTACGTCAAGCAGCGATCGGATCGTTTCAACCACTTCCTCGACCGCATGATCCTCAAGAT
GCCGATCTTCGGCCCCCTGACGGAAAAAGCCGCCATTGCGCGATTCTCACGCACCCTTGCCACCATGTTCTCGGCCGGTG
TACCCCTCGTCGAAGCCATGCCGCCGACCGCCGGCTCCACCGGCAACGCCGTCTTCAAGGATGCGGTCATGAACATGCGC
GACATCATTGCGGCGGGCTCGCCCCTGAAGGTCGGGATGCAGCAGTCCAAGCTGTTCCCCGTGATGGTCGTTCAGATGGT
CGCCATCGGTGAAGAGACCGGCGCACTGGACACCATGCTGGGCAAGGTCGCCGACATGTACGAGGAAGAGGTCGACAACA
TGGTCGACGCCATGTCCAGTTTGATGGAACCGATGATCATGGCATTCCTCGGCGTCGTTGTCGGCGGCCTCGTCATTGCC
ATGTACCTGCCGATTTTCAAACTCGGATCCGTGGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(78-201)

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A191ZG85

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

55.051

96.117

0.529
  pilC Acinetobacter baylyi ADP1

52.261

96.602

0.505
  pilC Pseudomonas stutzeri DSM 10701

52.13

96.845

0.505
  pilC Acinetobacter baumannii D1279779

51.637

96.359

0.498
  pilC Vibrio campbellii strain DS40M4

44.389

97.33

0.432
  pilG Neisseria meningitidis 44/76-A

43.103

98.544

0.425
  pilG Neisseria gonorrhoeae MS11

42.857

98.544

0.422
  pilC Vibrio cholerae strain A1552

43.939

96.117

0.422
  pilC Thermus thermophilus HB27

40.852

96.845

0.396

Multiple sequence alignment