Detailed information NTG0185420

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: A9404_RS05450
• Genome accession: NZ_CP016027
• Coordinates: 1232988..1233878 (+)
• Length: 296 a.a.
• NCBI ID: WP_066099259.1
• Uniprot ID: A0A191ZGB7
• Organism: Halothiobacillus diazotrophicus strain LS2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1227988..1238878

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A9404_RS05430 (A9404_05430)	-	1228364..1229386 (+)	1023	WP_066099255.1	MerR family transcriptional regulator	-
A9404_RS05435 (A9404_05435)	-	1229460..1229918 (+)	459	WP_066099256.1	PAS domain-containing protein	-
A9404_RS05440 (A9404_05440)	pilB	1230000..1231700 (+)	1701	WP_066099257.1	type IV-A pilus assembly ATPase PilB	Machinery gene
A9404_RS05445 (A9404_05445)	pilC	1231712..1232950 (+)	1239	WP_066099258.1	type II secretion system F family protein	Machinery gene
A9404_RS05450 (A9404_05450)	pilD	1232988..1233878 (+)	891	WP_066099259.1	A24 family peptidase	Machinery gene
A9404_RS05455 (A9404_05455)	coaE	1233922..1234545 (+)	624	WP_082922762.1	dephospho-CoA kinase	-
A9404_RS05460 (A9404_05460)	-	1234561..1234992 (-)	432	WP_066099261.1	hypothetical protein	-
A9404_RS05465 (A9404_05465)	-	1235080..1236120 (+)	1041	WP_066099262.1	folate-binding protein YgfZ	-
A9404_RS05470 (A9404_05470)	-	1236264..1237055 (+)	792	WP_066099263.1	thiazole synthase	-
A9404_RS05475 (A9404_05475)	trmB	1237088..1237813 (+)	726	WP_066099264.1	tRNA (guanosine(46)-N7)-methyltransferase TrmB	-
A9404_RS05480 (A9404_05480)	-	1237818..1238099 (+)	282	WP_066099265.1	YrhK family protein	-

Sequence

Protein

• Length: 296 a.a.
• Molecular weight: 32478.91 Da
• Isoelectric Point: 8.0611

>NTDB_id=185420 A9404_RS05450 WP_066099259.1 1232988..1233878(+) (pilD) [Halothiobacillus diazotrophicus strain LS2]
MTLLATWAAFFTEYPAVWLGFAFILGLLIGSFLNVVIHRLPRMMQAEWHRDCRELLELPPEPDTASPFNLLHPRSACPHC
QTPIKSWQNIPLISWLLLRGKCAQCGHAISMQYPLIELLSGGLTLLAAWQFGPGATAVAVWVLGWVLIAAAIIDLKTTLL
PDVLTLPLLWLGLLLALTGIGTTVTLKDAVLGAMVGYLSLWSIYWLFKLTTGREGMGYGDFKLLAALGAWLGWHQLPLVL
LLSAGVGAVIGIGMILFLKHDKRIPIPFGPYLASAGLLAYYLGMPILDLYLGAHRF

Nucleotide

• Length: 891 bp

>NTDB_id=185420 A9404_RS05450 WP_066099259.1 1232988..1233878(+) (pilD) [Halothiobacillus diazotrophicus strain LS2]
ATGACGCTACTTGCCACGTGGGCTGCCTTTTTCACCGAATATCCAGCCGTGTGGCTGGGGTTCGCATTCATTCTCGGACT
GCTGATCGGCAGCTTTCTCAACGTCGTGATTCACCGCCTGCCCCGCATGATGCAGGCAGAGTGGCACCGGGATTGCCGGG
AACTGCTCGAACTGCCCCCCGAGCCCGATACGGCTTCTCCATTCAATCTCCTGCATCCGCGCTCGGCCTGCCCTCATTGC
CAGACACCCATCAAAAGCTGGCAGAACATCCCGCTCATCAGCTGGTTGCTGCTGCGCGGGAAATGCGCCCAATGCGGCCA
TGCCATCAGCATGCAGTACCCCCTGATCGAGCTGCTGAGCGGTGGACTGACCCTATTGGCGGCCTGGCAATTCGGCCCCG
GTGCCACGGCGGTCGCCGTCTGGGTACTCGGGTGGGTACTGATCGCAGCAGCGATCATCGACCTGAAAACCACGCTCCTG
CCGGACGTACTCACCCTTCCGCTGCTCTGGCTGGGGCTTTTGCTGGCGCTTACCGGGATCGGTACAACCGTCACCCTCAA
GGATGCCGTCCTGGGTGCCATGGTCGGCTATCTGTCGCTCTGGTCGATCTACTGGCTGTTCAAGCTGACCACCGGTCGCG
AGGGCATGGGATATGGAGACTTCAAATTGCTGGCGGCCCTGGGCGCCTGGCTGGGCTGGCACCAGCTTCCGCTCGTATTA
CTGCTTTCCGCAGGCGTTGGTGCCGTCATCGGCATCGGGATGATTCTCTTCCTGAAGCACGACAAGCGTATTCCCATCCC
CTTCGGCCCCTATCTGGCCAGCGCGGGCCTCCTGGCCTACTACCTGGGGATGCCCATCCTGGATTTGTATCTCGGCGCGC
ATCGGTTTTAG

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (24-131)

• Type IV leader peptidase family (Peptidase_A24) (141-252)

3D structure

• AlphaFold DB: A0A191ZGB7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.737	96.284	0.527
pilD	Vibrio campbellii strain DS40M4	52.817	95.946	0.507
pilD	Acinetobacter nosocomialis M2	49.65	96.622	0.48
pilD	Acinetobacter baumannii D1279779	49.301	96.622	0.476
pilD	Neisseria gonorrhoeae MS11	50.196	86.149	0.432