Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A9404_RS05440 Genome accession   NZ_CP016027
Coordinates   1230000..1231700 (+) Length   566 a.a.
NCBI ID   WP_066099257.1    Uniprot ID   A0A191ZGB4
Organism   Halothiobacillus diazotrophicus strain LS2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1225000..1236700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9404_RS05415 (A9404_05415) rimI 1225365..1225829 (-) 465 WP_066099252.1 ribosomal protein S18-alanine N-acetyltransferase -
  A9404_RS05420 (A9404_05420) - 1225863..1226375 (-) 513 WP_156521249.1 hypothetical protein -
  A9404_RS05425 (A9404_05425) - 1226386..1227939 (-) 1554 WP_066099254.1 2-isopropylmalate synthase -
  A9404_RS05430 (A9404_05430) - 1228364..1229386 (+) 1023 WP_066099255.1 MerR family transcriptional regulator -
  A9404_RS05435 (A9404_05435) - 1229460..1229918 (+) 459 WP_066099256.1 PAS domain-containing protein -
  A9404_RS05440 (A9404_05440) pilB 1230000..1231700 (+) 1701 WP_066099257.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A9404_RS05445 (A9404_05445) pilC 1231712..1232950 (+) 1239 WP_066099258.1 type II secretion system F family protein Machinery gene
  A9404_RS05450 (A9404_05450) pilD 1232988..1233878 (+) 891 WP_066099259.1 A24 family peptidase Machinery gene
  A9404_RS05455 (A9404_05455) coaE 1233922..1234545 (+) 624 WP_082922762.1 dephospho-CoA kinase -
  A9404_RS05460 (A9404_05460) - 1234561..1234992 (-) 432 WP_066099261.1 hypothetical protein -
  A9404_RS05465 (A9404_05465) - 1235080..1236120 (+) 1041 WP_066099262.1 folate-binding protein YgfZ -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 61793.02 Da        Isoelectric Point: 6.2929

>NTDB_id=185418 A9404_RS05440 WP_066099257.1 1230000..1231700(+) (pilB) [Halothiobacillus diazotrophicus strain LS2]
MTHPPITGLLAALVKTGVIDAPRATEIQEKAKASKESTIQALMGNGISAAQIARAASRNFGLPLVDLEAFDTSELPIKLV
PAKLLENGNALPLRQRGRRLTVALADPSDLGIIESFRFSTNLTIDTVVAEAPKIGRLLEEAANHSMDALTADLDDDLANI
QIEGGEDSAAAEQSDDPLQDDVVVKFVTKVLTDAIRRKASDIHIEPYEKQFRIRFRIDGILTEMPAPPRTLADRIVSRVK
VMSRMDISERRIPQDGRIKLKLSAGRSIDFRVNSCPTLFGEKIVLRILDPSSAQLGIDSLGYEAEQKELYLKALNRPYGM
ILVTGPTGSGKTVSLYTGLNILNTMDRNISTAEDPAEIVVPGINQVNVNARVGLTFASALRAFLRQDPDVIMVGEIRDLE
TAEIAIKAAQTGHLVLSTLHTNDAPQTLTRLLNMGVPGYNIASSVNLIIAQRLARRLCNACKKVDQVPEEELIREGFKPE
DVHNGLKLFKAVGCDQCTNGYKGRVGIYQVMPVSEEMGRIIMRGGNSMELAEQARKENIPDLRSSGLRKVQAGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=185418 A9404_RS05440 WP_066099257.1 1230000..1231700(+) (pilB) [Halothiobacillus diazotrophicus strain LS2]
ATGACCCACCCGCCCATTACCGGCCTTCTGGCAGCCCTGGTGAAAACCGGTGTTATCGATGCCCCGCGCGCCACGGAAAT
TCAGGAAAAGGCCAAAGCCAGCAAGGAATCCACCATCCAGGCCCTGATGGGCAACGGGATTTCGGCCGCCCAGATCGCCC
GCGCCGCTTCGCGAAATTTCGGTCTACCCCTTGTTGACCTCGAGGCATTCGATACCTCCGAACTGCCCATCAAGCTCGTT
CCGGCAAAACTGCTGGAAAACGGCAACGCGCTGCCTTTGCGTCAACGCGGACGTCGTCTGACCGTCGCACTGGCCGACCC
GTCGGACCTCGGCATCATCGAAAGCTTCCGCTTCAGCACCAACCTGACCATCGACACGGTCGTCGCCGAAGCGCCGAAAA
TCGGTCGTCTGCTTGAAGAGGCCGCCAATCATTCGATGGATGCCCTAACGGCAGATCTCGATGATGATCTGGCCAACATC
CAGATCGAAGGCGGCGAGGACAGCGCTGCAGCCGAACAGAGCGACGATCCGCTTCAGGACGACGTGGTGGTCAAGTTCGT
GACCAAGGTCCTGACCGACGCCATTCGGCGGAAGGCCTCGGACATTCATATCGAACCTTACGAAAAGCAGTTTCGGATCC
GTTTTCGGATCGACGGCATCCTGACGGAAATGCCTGCCCCACCGCGCACGCTGGCCGACCGGATCGTCTCCCGGGTCAAG
GTCATGAGCCGCATGGACATTTCCGAGCGCCGCATTCCACAGGATGGCCGGATCAAGCTGAAGCTGTCCGCCGGTCGTTC
GATCGACTTCCGGGTCAACTCCTGCCCCACGCTGTTCGGCGAAAAGATCGTTCTTCGTATCCTCGATCCCAGCAGCGCGC
AACTCGGAATCGATTCCCTGGGTTACGAAGCGGAGCAGAAGGAACTCTATCTCAAGGCACTCAATCGCCCCTACGGGATG
ATTCTCGTCACCGGCCCGACCGGTTCCGGTAAGACCGTCTCGCTCTATACCGGCCTGAACATCCTGAACACCATGGACCG
GAACATTTCGACCGCGGAAGATCCTGCAGAAATCGTGGTACCCGGCATCAACCAGGTCAACGTCAACGCCCGGGTGGGAC
TGACCTTCGCCTCGGCCCTTCGCGCCTTTCTGCGTCAGGATCCGGACGTCATCATGGTGGGCGAGATCCGGGATCTGGAA
ACGGCCGAAATCGCCATCAAGGCGGCACAGACCGGTCACCTCGTGCTTTCGACCCTACACACCAACGATGCGCCGCAAAC
CCTCACCCGCCTGCTCAACATGGGGGTTCCCGGTTACAACATCGCCTCATCGGTGAACCTGATCATCGCACAGCGCTTGG
CGCGGCGTCTGTGCAATGCCTGCAAGAAGGTCGACCAGGTCCCCGAGGAAGAGTTGATTCGGGAAGGATTCAAACCCGAG
GATGTCCACAACGGCCTGAAGCTGTTCAAGGCCGTCGGCTGCGACCAGTGCACCAATGGCTACAAGGGCCGCGTGGGCAT
CTATCAGGTCATGCCCGTTTCCGAGGAAATGGGGCGCATCATCATGCGTGGCGGCAACTCGATGGAGCTGGCCGAACAGG
CGCGCAAAGAAAACATTCCCGACCTGAGAAGCTCAGGACTTCGTAAGGTTCAGGCCGGGGTGACCAGCCTCGAAGAAATC
AACCGGGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-142)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A191ZGB4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.106

100

0.553
  pilB Acinetobacter baumannii D1279779

54.225

100

0.544
  pilB Legionella pneumophila strain ERS1305867

53.439

100

0.535
  pilB Vibrio cholerae strain A1552

49.033

100

0.493
  pilF Neisseria gonorrhoeae MS11

48.668

99.47

0.484
  pilB Vibrio campbellii strain DS40M4

48.057

100

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

47.611

99.823

0.475
  pilB/pilB1 Synechocystis sp. PCC 6803

37.643

100

0.406

Multiple sequence alignment