Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   THL1_RS05075 Genome accession   NZ_CP015992
Coordinates   1093949..1095166 (-) Length   405 a.a.
NCBI ID   WP_069082243.1    Uniprot ID   A0A1B3E208
Organism   Pseudomonas sp. TCU-HL1 isolate Hualien Taiwan     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1088949..1100166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THL1_RS05030 (THL1_1006) - 1089319..1089492 (-) 174 WP_069082234.1 DUF3094 family protein -
  THL1_RS05035 (THL1_1007) - 1089577..1090203 (-) 627 WP_069082235.1 DUF1780 domain-containing protein -
  THL1_RS05040 (THL1_1008) - 1090338..1090760 (+) 423 WP_069082236.1 GNAT family N-acetyltransferase -
  THL1_RS05045 (THL1_1009) - 1090765..1091232 (+) 468 WP_069082237.1 FAD/FMN-containing dehydrogenase -
  THL1_RS05050 (THL1_1010) - 1091229..1091918 (+) 690 WP_069082238.1 energy-coupling factor ABC transporter permease -
  THL1_RS05055 (THL1_1011) - 1092044..1092259 (+) 216 WP_069082239.1 hypothetical protein -
  THL1_RS05060 (THL1_1012) yacG 1092269..1092469 (-) 201 WP_069082240.1 DNA gyrase inhibitor YacG -
  THL1_RS05065 (THL1_1013) coaE 1092466..1093077 (-) 612 WP_069082241.1 dephospho-CoA kinase -
  THL1_RS05070 (THL1_1014) pilD 1093074..1093946 (-) 873 WP_069082242.1 prepilin peptidase Machinery gene
  THL1_RS05075 (THL1_1015) pilC 1093949..1095166 (-) 1218 WP_069082243.1 type II secretion system F family protein Machinery gene
  THL1_RS05080 (THL1_1016) pilB 1095169..1096875 (-) 1707 WP_069082244.1 type IV-A pilus assembly ATPase PilB Machinery gene
  THL1_RS05085 (THL1_1017) pilA 1097141..1097548 (+) 408 WP_069086413.1 pilin Machinery gene
  THL1_RS05090 (THL1_1018) - 1097627..1099249 (+) 1623 WP_069082245.1 tetratricopeptide repeat protein -
  THL1_RS05100 (THL1_1019) - 1099466..1099693 (-) 228 WP_069082246.1 hypothetical protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44022.88 Da        Isoelectric Point: 9.8473

>NTDB_id=184968 THL1_RS05075 WP_069082243.1 1093949..1095166(-) (pilC) [Pseudomonas sp. TCU-HL1 isolate Hualien Taiwan]
MAEKALKTSTFAWEGTDKKGGKVKGELTGQNPALVKAQLRKQGINPTKVRKKSASLLNTGKKIKPMDIALFTRQLATMMK
AGVPLMQSFDIIGEGFEKPAMRTLVNEVKQEVSSGNSLANSLRKKPDYFDELYCNLVDSGEQAGALETLLDRIATYKEKT
EALKAKIKKAMNYPIAVVVVAIIVTAILLIKVVPQFKTVFANFGAELPAFTLMVIGISEILQEWWFIVLLGFFAAGFLIQ
HTHRKSEKFRDWVDRTVLKLPIVGDILYKSAVARFARTLATTFAAGVPLVDALDSVAGASGNVVFKNAVLKVKSDVSTGM
QLNFSMRTTGVFPAMAVQMTAIGEESGALDDMLDKVASFYEQEVDNAVDGLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGAVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=184968 THL1_RS05075 WP_069082243.1 1093949..1095166(-) (pilC) [Pseudomonas sp. TCU-HL1 isolate Hualien Taiwan]
ATGGCGGAAAAAGCGCTTAAAACCAGTACCTTCGCCTGGGAAGGCACCGACAAGAAAGGCGGAAAGGTCAAGGGCGAACT
CACAGGGCAGAACCCGGCGCTTGTGAAAGCGCAGTTGCGCAAACAAGGTATCAACCCGACCAAGGTGCGCAAGAAGTCCG
CTTCTCTGCTGAATACAGGGAAGAAGATCAAGCCCATGGATATCGCACTGTTCACCCGTCAGTTGGCAACCATGATGAAG
GCAGGTGTCCCGCTGATGCAGTCCTTCGACATTATTGGCGAAGGTTTCGAGAAGCCGGCAATGCGTACGCTGGTGAATGA
AGTCAAGCAGGAGGTTTCCTCGGGCAATAGCCTGGCAAACTCCCTGCGCAAGAAACCGGATTACTTCGACGAACTGTATT
GCAATCTAGTGGACTCAGGCGAACAGGCAGGTGCGCTGGAAACGCTGCTTGACCGGATTGCCACCTATAAGGAAAAGACC
GAGGCCCTGAAGGCGAAGATCAAGAAGGCGATGAACTACCCAATTGCGGTAGTCGTGGTTGCGATCATCGTGACAGCAAT
TCTTCTGATCAAAGTCGTACCGCAGTTCAAAACAGTATTCGCGAACTTCGGTGCTGAGCTTCCGGCCTTTACCCTGATGG
TCATCGGGATATCCGAGATACTCCAAGAATGGTGGTTCATCGTTTTACTTGGCTTCTTTGCGGCTGGTTTTCTAATCCAG
CATACTCATCGAAAGTCTGAAAAGTTCCGCGACTGGGTTGATCGCACCGTTCTCAAACTGCCCATCGTCGGCGACATTCT
TTACAAGTCGGCGGTGGCCCGATTCGCCCGGACACTCGCTACCACCTTCGCCGCTGGTGTTCCACTTGTGGATGCCCTGG
ACTCGGTGGCTGGCGCCAGCGGCAACGTTGTCTTCAAAAACGCTGTGCTAAAGGTTAAATCTGATGTCTCCACCGGCATG
CAGCTCAACTTCTCCATGCGCACCACCGGCGTCTTCCCGGCCATGGCCGTACAAATGACTGCCATCGGCGAAGAGTCCGG
CGCGCTGGACGATATGCTCGACAAGGTCGCCAGCTTCTATGAACAGGAAGTAGATAACGCCGTCGATGGCCTGACCGCGC
TTATGGAACCCATGATTATGGCCGTACTCGGCGTACTGGTCGGCGGGCTGATCATCGCCATGTACCTGCCCATTTTCCAA
CTGGGCGCAGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B3E208

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

80

100

0.8
  pilC Acinetobacter baylyi ADP1

61.275

100

0.617
  pilC Acinetobacter baumannii D1279779

60.247

100

0.602
  pilC Legionella pneumophila strain ERS1305867

53.133

98.519

0.523
  pilG Neisseria gonorrhoeae MS11

45.792

99.753

0.457
  pilG Neisseria meningitidis 44/76-A

45.025

99.259

0.447
  pilC Vibrio cholerae strain A1552

41.523

100

0.417
  pilC Vibrio campbellii strain DS40M4

41.162

97.778

0.402
  pilC Thermus thermophilus HB27

37.25

98.765

0.368

Multiple sequence alignment