Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   THL1_RS05080 Genome accession   NZ_CP015992
Coordinates   1095169..1096875 (-) Length   568 a.a.
NCBI ID   WP_069082244.1    Uniprot ID   -
Organism   Pseudomonas sp. TCU-HL1 isolate Hualien Taiwan     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1090169..1101875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THL1_RS05040 (THL1_1008) - 1090338..1090760 (+) 423 WP_069082236.1 GNAT family N-acetyltransferase -
  THL1_RS05045 (THL1_1009) - 1090765..1091232 (+) 468 WP_069082237.1 FAD/FMN-containing dehydrogenase -
  THL1_RS05050 (THL1_1010) - 1091229..1091918 (+) 690 WP_069082238.1 energy-coupling factor ABC transporter permease -
  THL1_RS05055 (THL1_1011) - 1092044..1092259 (+) 216 WP_069082239.1 hypothetical protein -
  THL1_RS05060 (THL1_1012) yacG 1092269..1092469 (-) 201 WP_069082240.1 DNA gyrase inhibitor YacG -
  THL1_RS05065 (THL1_1013) coaE 1092466..1093077 (-) 612 WP_069082241.1 dephospho-CoA kinase -
  THL1_RS05070 (THL1_1014) pilD 1093074..1093946 (-) 873 WP_069082242.1 prepilin peptidase Machinery gene
  THL1_RS05075 (THL1_1015) pilC 1093949..1095166 (-) 1218 WP_069082243.1 type II secretion system F family protein Machinery gene
  THL1_RS05080 (THL1_1016) pilB 1095169..1096875 (-) 1707 WP_069082244.1 type IV-A pilus assembly ATPase PilB Machinery gene
  THL1_RS05085 (THL1_1017) pilA 1097141..1097548 (+) 408 WP_069086413.1 pilin Machinery gene
  THL1_RS05090 (THL1_1018) - 1097627..1099249 (+) 1623 WP_069082245.1 tetratricopeptide repeat protein -
  THL1_RS05100 (THL1_1019) - 1099466..1099693 (-) 228 WP_069082246.1 hypothetical protein -
  THL1_RS05105 (THL1_1020) nadC 1100070..1100918 (-) 849 WP_069082247.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62702.80 Da        Isoelectric Point: 5.3332

>NTDB_id=184969 THL1_RS05080 WP_069082244.1 1095169..1096875(-) (pilB) [Pseudomonas sp. TCU-HL1 isolate Hualien Taiwan]
MNETAPTLSGLAKQLVLTNLLDERSALQAHTQAQRNKIPLITYLVQNKLLKSRDLAEMAAEQFGVAFLDLSSIDRDSQPK
ELVSEKLIRQHRALPLWRRGNKLFVAISDPTNHQAVTDIQFSTGLNTEAILVEDDKLGDAIEKFFDSANSGMEDLGDVDL
DGVDVEAVDDDKKDSPGGVDADDAPVVRFVNKMLLDAIRGGSSDLHFEPYERSYRVRFRTDGMLHEVAKPPIQLASRIAA
RLKVMAALDISERRKPQDGRIKMKISKVKSIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEEEQKDLYMVALKQP
QGMILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPKQGLDFAQALRAFLRQDPDVIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNSAAETLTRMRNMGVPAFNIATSVNLIIAQRLARKLCAHCKKELNIPREALIEEGF
PEAKVGTFTLYGPVGCENCNQGYKGRVGIYEVVKITPAMQQLIMEEGNSIQIAQQARRDGFNDLRTSGLVKAMNGITSLE
EVNRVTKD

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=184969 THL1_RS05080 WP_069082244.1 1095169..1096875(-) (pilB) [Pseudomonas sp. TCU-HL1 isolate Hualien Taiwan]
ATGAACGAGACCGCCCCCACGCTATCCGGCCTAGCCAAACAGCTTGTCCTGACCAATTTGCTGGATGAGCGAAGCGCCCT
CCAGGCCCATACCCAGGCGCAGCGCAACAAGATTCCGCTGATCACCTACCTGGTGCAGAACAAGCTGCTGAAGAGTCGGG
ATCTCGCGGAAATGGCGGCGGAGCAATTCGGTGTCGCCTTCCTGGACTTGAGCAGCATCGACAGGGATTCCCAGCCTAAG
GAGCTTGTTAGCGAAAAGCTGATTCGCCAGCACCGGGCCCTGCCCCTGTGGCGGCGCGGCAACAAGCTGTTCGTCGCCAT
ATCCGACCCGACTAATCACCAGGCCGTTACCGATATCCAGTTCAGCACCGGCCTGAACACCGAGGCCATTCTGGTCGAGG
ACGACAAGCTCGGCGATGCCATCGAGAAGTTCTTCGACAGCGCCAACAGCGGGATGGAAGACCTGGGCGACGTCGACCTG
GATGGCGTGGACGTTGAAGCTGTCGACGACGACAAGAAGGACAGCCCCGGCGGTGTGGATGCCGACGACGCCCCGGTGGT
GCGTTTCGTGAATAAGATGCTGCTGGACGCCATTCGCGGCGGTTCCTCAGACCTGCACTTCGAGCCCTACGAGCGCTCCT
ACCGTGTTCGCTTTCGTACCGATGGCATGCTGCATGAAGTGGCCAAGCCGCCCATCCAACTGGCCAGCCGTATTGCTGCG
CGCCTCAAGGTGATGGCTGCTCTCGACATTTCCGAGCGCCGCAAGCCCCAGGACGGCCGGATCAAGATGAAGATATCCAA
GGTCAAGTCCATCGACTTCCGCGTCAACACCCTGCCAACGCTCTGGGGCGAGAAGATCGTGATGCGGATTCTCGACCCCA
CCAGCGCGCAGATGGGTATCGATGCCCTGGGCTATGAAGAGGAACAGAAAGACCTGTACATGGTGGCCCTGAAGCAGCCC
CAGGGCATGATCCTAGTGACCGGCCCTACCGGTTCGGGCAAAACCGTTTCTCTGTACACCGGCCTCAACATTCTCAATAC
CGTGGATATCAATATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCCA
AGCAGGGCCTGGACTTCGCCCAAGCCCTGCGCGCCTTCCTGCGCCAGGATCCGGACGTGATCATGGTGGGCGAGATCCGT
GACCTGGAAACCGCCGAAATCGCCATCAAGGCCGCGCAAACCGGCCATATGGTGCTGTCCACCCTGCACACCAACAGCGC
AGCGGAAACCCTTACCCGTATGCGCAATATGGGTGTGCCTGCCTTCAACATTGCCACCTCGGTCAACCTGATCATCGCCC
AGCGCCTGGCACGCAAATTGTGTGCGCACTGCAAGAAGGAGCTGAACATTCCACGGGAAGCGCTGATCGAGGAAGGCTTC
CCCGAAGCGAAGGTCGGCACCTTCACGCTCTATGGGCCGGTCGGCTGCGAAAACTGCAACCAGGGCTACAAGGGCCGAGT
GGGTATTTATGAAGTGGTTAAAATCACGCCGGCCATGCAGCAGCTTATTATGGAAGAAGGCAACTCCATCCAGATCGCCC
AGCAGGCGCGCCGCGATGGCTTCAATGACCTGCGAACCTCCGGCCTGGTGAAAGCCATGAATGGCATCACCAGCCTTGAG
GAAGTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.524

100

0.586
  pilB Acinetobacter baylyi ADP1

58.201

99.824

0.581
  pilB Legionella pneumophila strain ERS1305867

57.672

99.824

0.576
  pilB Vibrio parahaemolyticus RIMD 2210633

49.645

99.296

0.493
  pilF Neisseria gonorrhoeae MS11

49.378

99.12

0.489
  pilB Vibrio cholerae strain A1552

52.481

92.254

0.484
  pilB Vibrio campbellii strain DS40M4

48.668

99.12

0.482
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.13

93.134

0.364

Multiple sequence alignment