Detailed information NTG0184967

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: THL1_RS05070
• Genome accession: NZ_CP015992
• Coordinates: 1093074..1093946 (-)
• Length: 290 a.a.
• NCBI ID: WP_069082242.1
• Uniprot ID: A0A1B3E1Z6
• Organism: Pseudomonas sp. TCU-HL1 isolate Hualien Taiwan
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1088074..1098946

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
THL1_RS05030 (THL1_1006)	-	1089319..1089492 (-)	174	WP_069082234.1	DUF3094 family protein	-
THL1_RS05035 (THL1_1007)	-	1089577..1090203 (-)	627	WP_069082235.1	DUF1780 domain-containing protein	-
THL1_RS05040 (THL1_1008)	-	1090338..1090760 (+)	423	WP_069082236.1	GNAT family N-acetyltransferase	-
THL1_RS05045 (THL1_1009)	-	1090765..1091232 (+)	468	WP_069082237.1	FAD/FMN-containing dehydrogenase	-
THL1_RS05050 (THL1_1010)	-	1091229..1091918 (+)	690	WP_069082238.1	energy-coupling factor ABC transporter permease	-
THL1_RS05055 (THL1_1011)	-	1092044..1092259 (+)	216	WP_069082239.1	hypothetical protein	-
THL1_RS05060 (THL1_1012)	yacG	1092269..1092469 (-)	201	WP_069082240.1	DNA gyrase inhibitor YacG	-
THL1_RS05065 (THL1_1013)	coaE	1092466..1093077 (-)	612	WP_069082241.1	dephospho-CoA kinase	-
THL1_RS05070 (THL1_1014)	pilD	1093074..1093946 (-)	873	WP_069082242.1	prepilin peptidase	Machinery gene
THL1_RS05075 (THL1_1015)	pilC	1093949..1095166 (-)	1218	WP_069082243.1	type II secretion system F family protein	Machinery gene
THL1_RS05080 (THL1_1016)	pilB	1095169..1096875 (-)	1707	WP_069082244.1	type IV-A pilus assembly ATPase PilB	Machinery gene
THL1_RS05085 (THL1_1017)	pilA	1097141..1097548 (+)	408	WP_069086413.1	pilin	Machinery gene

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31834.86 Da
• Isoelectric Point: 7.0714

>NTDB_id=184967 THL1_RS05070 WP_069082242.1 1093074..1093946(-) (pilD) [Pseudomonas sp. TCU-HL1 isolate Hualien Taiwan]
MQLIDFLASHALAFVFCALLLGLLIGSFLNVVIHRLPKMMMLDWQEQAREALSLPAEPKGPVFNLVLPNSRCPHCSHEIR
AWENIPVVSYLALGGKCSSCKAPISKRYPLVELACGALSAYVAWHFGFGWQAGAFLVLTWGLLAMSMIDVDHQLLPDVLV
LPLLWLGLILNSQSLFTGLEDALWGAVAGYLSLWSVYWLFKLVTGKEGMGHGDFKLLAMLGAWGGWQVLPLTILLSSLVG
AILGVIMLRLRNQDTATPIPFGPYLAIAGWIALLWGDQITATYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=184967 THL1_RS05070 WP_069082242.1 1093074..1093946(-) (pilD) [Pseudomonas sp. TCU-HL1 isolate Hualien Taiwan]
ATGCAATTGATCGACTTTCTGGCCAGCCATGCGCTGGCCTTTGTTTTCTGCGCGCTGTTGCTCGGACTGCTGATCGGCAG
CTTCCTCAACGTGGTCATCCACCGCCTGCCCAAGATGATGATGCTGGACTGGCAGGAGCAGGCGCGCGAAGCCCTGAGCC
TGCCGGCCGAGCCCAAGGGGCCGGTGTTCAACCTGGTGCTACCGAACTCCCGCTGCCCCCATTGCAGCCATGAGATTCGC
GCCTGGGAGAACATCCCGGTGGTGAGTTACCTGGCGCTGGGGGGCAAGTGTTCGTCGTGCAAGGCCCCCATCAGCAAGCG
CTATCCGCTGGTCGAGCTGGCCTGCGGCGCGCTGTCGGCCTATGTGGCCTGGCATTTCGGATTCGGCTGGCAGGCCGGCG
CCTTCCTGGTGCTGACCTGGGGCCTGCTGGCGATGAGCATGATCGACGTCGATCACCAACTACTGCCGGATGTGCTGGTA
CTGCCGCTGCTGTGGTTGGGCCTGATCCTCAACAGCCAGAGCCTGTTCACCGGCCTGGAGGATGCGCTGTGGGGCGCGGT
GGCCGGTTACCTGAGCCTGTGGTCGGTGTACTGGCTGTTCAAGCTGGTGACGGGCAAGGAAGGCATGGGCCACGGCGACT
TCAAGCTGCTGGCAATGCTGGGCGCCTGGGGTGGCTGGCAGGTGCTGCCGCTGACCATTCTGCTGTCCTCCCTGGTGGGC
GCCATTCTGGGTGTGATCATGCTGCGCCTGCGGAACCAGGACACGGCCACGCCCATACCCTTCGGTCCGTACCTGGCCAT
TGCCGGCTGGATTGCGCTGCTCTGGGGTGATCAAATAACGGCGACCTATCTGCAGTTCGCCGGTTTCAAATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-245)

3D structure

• AlphaFold DB: A0A1B3E1Z6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.435	95.172	0.528
pilD	Vibrio campbellii strain DS40M4	53.48	94.138	0.503
pilD	Acinetobacter baumannii D1279779	50.694	99.31	0.503
pilD	Acinetobacter nosocomialis M2	50	99.31	0.497
pilD	Neisseria gonorrhoeae MS11	49.81	90.69	0.452