Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   A4W82_RS07255 Genome accession   NZ_CP015487
Coordinates   1398790..1399680 (-) Length   296 a.a.
NCBI ID   WP_251925659.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain TMW 1.46     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1393790..1404680
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W82_RS07225 (A4W82_07125) - 1394857..1395867 (-) 1011 WP_025016388.1 class I SAM-dependent methyltransferase -
  A4W82_RS07230 (A4W82_07130) - 1395991..1396329 (-) 339 WP_056947123.1 hypothetical protein -
  A4W82_RS07235 (A4W82_07135) comGF 1396292..1396804 (-) 513 WP_251925653.1 competence type IV pilus minor pilin ComGF Machinery gene
  A4W82_RS07240 (A4W82_07140) comGD 1396779..1397522 (-) 744 WP_251925655.1 type II secretion system protein Machinery gene
  A4W82_RS07245 (A4W82_07145) comGC 1397494..1397793 (-) 300 WP_076645268.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  A4W82_RS07250 (A4W82_07150) comGB 1397790..1398797 (-) 1008 WP_251925657.1 type II secretion system F family protein Machinery gene
  A4W82_RS07255 (A4W82_07155) comGA 1398790..1399680 (-) 891 WP_251925659.1 competence type IV pilus ATPase ComGA Machinery gene
  A4W82_RS07260 (A4W82_07160) - 1399795..1400526 (-) 732 WP_011375002.1 YebC/PmpR family DNA-binding transcriptional regulator -
  A4W82_RS07265 (A4W82_07165) - 1400617..1401117 (-) 501 WP_016265379.1 VanZ family protein -
  A4W82_RS07270 (A4W82_07170) - 1401214..1401588 (+) 375 WP_011375004.1 hypothetical protein -
  A4W82_RS07280 (A4W82_07180) - 1401818..1402909 (-) 1092 WP_011375005.1 zinc ribbon domain-containing protein -
  A4W82_RS07285 (A4W82_07185) - 1403149..1403388 (+) 240 WP_251925661.1 cytochrome b5 domain-containing protein -
  A4W82_RS07290 (A4W82_07190) - 1403455..1404468 (-) 1014 WP_025016377.1 putative sulfate exporter family transporter -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 32988.91 Da        Isoelectric Point: 6.3456

>NTDB_id=179997 A4W82_RS07255 WP_251925659.1 1398790..1399680(-) (comGA) [Latilactobacillus sakei strain TMW 1.46]
MSTEEKLTTLLKYCAKQRVQDLYFAPTIGGWRLTERRQSDLVTNQELDKPTGTRYLNRLKYMAGMDISETRRAQTGRSEL
QLADQIIYLRLATVGDFLNRESLVIRFIYPIGAIYHCDDQQILAQLTQMSRKAGLILFAGPTGSGKTTSLYHLAQQSMSA
KMVVAIEDPIEIVAPEFLQLQVNDNAGLSYAELLKISLRLRPDTLIIGEIRDLETAQYAVSAALSGHLVLSTIHARSTQG
VVARLLDLGISELQLRACLTGIAYQSLVTKDDVVQAHYELLTGPDYFSKKEGDCDV

Nucleotide


Download         Length: 891 bp        

>NTDB_id=179997 A4W82_RS07255 WP_251925659.1 1398790..1399680(-) (comGA) [Latilactobacillus sakei strain TMW 1.46]
ATGTCAACTGAAGAAAAGCTAACAACTTTATTAAAGTATTGTGCTAAACAACGTGTTCAAGATCTTTATTTTGCACCGAC
AATAGGTGGCTGGCGATTGACGGAACGTCGTCAGAGCGATTTGGTCACCAATCAAGAATTGGATAAACCGACTGGAACGC
GTTATTTAAATCGACTGAAGTATATGGCGGGGATGGATATCAGCGAGACTCGGCGAGCGCAAACCGGCCGCTCGGAATTA
CAGCTAGCCGATCAAATAATTTATTTACGCCTAGCGACGGTCGGGGATTTTTTAAACCGCGAAAGCTTGGTCATTCGATT
TATCTATCCAATTGGTGCCATCTATCATTGTGATGATCAACAGATCTTGGCGCAATTAACGCAGATGAGTCGAAAGGCGG
GCTTAATTCTTTTCGCTGGGCCGACTGGTTCTGGCAAAACGACTAGCCTCTATCATCTGGCCCAACAATCAATGAGTGCC
AAGATGGTGGTTGCCATTGAAGATCCGATTGAAATTGTTGCACCTGAGTTTTTGCAACTGCAGGTTAATGATAATGCGGG
ATTGAGCTACGCCGAGCTATTAAAAATTAGTTTACGACTGCGACCGGATACGCTGATTATCGGTGAGATTCGCGATTTGG
AGACAGCCCAATATGCAGTTTCTGCGGCCTTAAGTGGCCACCTGGTATTGAGTACTATTCACGCTCGGAGTACACAGGGC
GTGGTCGCGCGCTTATTAGATCTTGGTATTTCAGAGCTGCAATTGCGGGCCTGTCTAACGGGAATTGCCTACCAATCGTT
GGTGACAAAAGACGACGTTGTGCAAGCCCATTATGAATTATTAACTGGTCCAGATTATTTCTCAAAAAAAGAAGGTGATT
GCGATGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(16-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Latilactobacillus sakei subsp. sakei 23K

97.297

100

0.973
  comGA Lactococcus lactis subsp. cremoris KW2

37.793

100

0.382
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

42.264

89.527

0.378
  comGA/cglA/cilD Streptococcus pneumoniae R6

42.264

89.527

0.378
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

42.264

89.527

0.378
  comGA/cglA/cilD Streptococcus pneumoniae D39

42.264

89.527

0.378
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

42.264

89.527

0.378
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

38.811

96.622

0.375
  comYA Streptococcus gordonii str. Challis substr. CH1

40.59

91.554

0.372

Multiple sequence alignment