Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   A4W82_RS07235 Genome accession   NZ_CP015487
Coordinates   1396292..1396804 (-) Length   170 a.a.
NCBI ID   WP_251925653.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain TMW 1.46     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1391292..1401804
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W82_RS07205 (A4W82_07105) - 1392218..1392583 (+) 366 WP_251925649.1 DUF805 domain-containing protein -
  A4W82_RS07215 (A4W82_07115) - 1393126..1393584 (-) 459 WP_035145008.1 hypothetical protein -
  A4W82_RS07220 (A4W82_07120) - 1393639..1394835 (-) 1197 WP_251925651.1 acetate kinase -
  A4W82_RS07225 (A4W82_07125) - 1394857..1395867 (-) 1011 WP_025016388.1 class I SAM-dependent methyltransferase -
  A4W82_RS07230 (A4W82_07130) - 1395991..1396329 (-) 339 WP_056947123.1 hypothetical protein -
  A4W82_RS07235 (A4W82_07135) comGF 1396292..1396804 (-) 513 WP_251925653.1 competence type IV pilus minor pilin ComGF Machinery gene
  A4W82_RS07240 (A4W82_07140) comGD 1396779..1397522 (-) 744 WP_251925655.1 type II secretion system protein Machinery gene
  A4W82_RS07245 (A4W82_07145) comGC 1397494..1397793 (-) 300 WP_076645268.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  A4W82_RS07250 (A4W82_07150) comGB 1397790..1398797 (-) 1008 WP_251925657.1 type II secretion system F family protein Machinery gene
  A4W82_RS07255 (A4W82_07155) comGA 1398790..1399680 (-) 891 WP_251925659.1 competence type IV pilus ATPase ComGA Machinery gene
  A4W82_RS07260 (A4W82_07160) - 1399795..1400526 (-) 732 WP_011375002.1 YebC/PmpR family DNA-binding transcriptional regulator -
  A4W82_RS07265 (A4W82_07165) - 1400617..1401117 (-) 501 WP_016265379.1 VanZ family protein -
  A4W82_RS07270 (A4W82_07170) - 1401214..1401588 (+) 375 WP_011375004.1 hypothetical protein -

Sequence

Protein


Download         Length: 170 a.a.        Molecular weight: 19720.66 Da        Isoelectric Point: 6.6472

>NTDB_id=179993 A4W82_RS07235 WP_251925653.1 1396292..1396804(-) (comGF) [Latilactobacillus sakei strain TMW 1.46]
MRLIGRPIKHSAFTLIEMVISMAILILTSQLLVATVGVLQKAIKVTQQQQNTINWHISMIQLDQFLADAYFTHHQDQDGS
KITFRKPDTNQSGEEAETVYYLKRMKNEMVVVSQLDGYMPLLGNLKQLQLDYQRPFVQIDATFTDQSRYQHEVFLEEQDE
QAKKKTIKTD

Nucleotide


Download         Length: 513 bp        

>NTDB_id=179993 A4W82_RS07235 WP_251925653.1 1396292..1396804(-) (comGF) [Latilactobacillus sakei strain TMW 1.46]
TTGCGATTAATTGGACGACCGATTAAGCATTCAGCGTTCACGCTAATTGAAATGGTGATTTCAATGGCGATTCTAATCCT
GACGAGTCAGTTACTGGTGGCAACGGTGGGTGTTTTACAAAAGGCAATTAAAGTGACACAACAACAGCAAAATACAATCA
ATTGGCATATCAGTATGATTCAGTTAGATCAGTTTTTAGCCGATGCGTATTTTACACATCATCAGGATCAAGATGGCTCC
AAAATCACTTTTCGAAAACCAGACACTAATCAGTCAGGGGAGGAAGCGGAGACTGTTTACTATTTAAAAAGGATGAAAAA
TGAAATGGTTGTTGTCAGCCAATTAGATGGGTATATGCCATTATTAGGCAACCTCAAGCAACTTCAACTTGACTATCAGC
GACCGTTTGTTCAAATCGATGCGACTTTTACGGATCAGAGTCGCTACCAACACGAAGTTTTCTTGGAGGAACAAGATGAA
CAGGCTAAAAAAAAGACGATTAAAACCGATTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(40-149)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Latilactobacillus sakei subsp. sakei 23K

98.235

100

0.982

Multiple sequence alignment