Detailed information NTG0179994

Bioinformatically predicted

Overview

• Name: comGD
• Type: Machinery gene
• Locus tag: A4W82_RS07240
• Genome accession: NZ_CP015487
• Coordinates: 1396779..1397522 (-)
• Length: 247 a.a.
• NCBI ID: WP_251925655.1
• Uniprot ID: -
• Organism: Latilactobacillus sakei strain TMW 1.46
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1391779..1402522

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A4W82_RS07205 (A4W82_07105)	-	1392218..1392583 (+)	366	WP_251925649.1	DUF805 domain-containing protein	-
A4W82_RS07215 (A4W82_07115)	-	1393126..1393584 (-)	459	WP_035145008.1	hypothetical protein	-
A4W82_RS07220 (A4W82_07120)	-	1393639..1394835 (-)	1197	WP_251925651.1	acetate kinase	-
A4W82_RS07225 (A4W82_07125)	-	1394857..1395867 (-)	1011	WP_025016388.1	class I SAM-dependent methyltransferase	-
A4W82_RS07230 (A4W82_07130)	-	1395991..1396329 (-)	339	WP_056947123.1	hypothetical protein	-
A4W82_RS07235 (A4W82_07135)	comGF	1396292..1396804 (-)	513	WP_251925653.1	competence type IV pilus minor pilin ComGF	Machinery gene
A4W82_RS07240 (A4W82_07140)	comGD	1396779..1397522 (-)	744	WP_251925655.1	type II secretion system protein	Machinery gene
A4W82_RS07245 (A4W82_07145)	comGC	1397494..1397793 (-)	300	WP_076645268.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
A4W82_RS07250 (A4W82_07150)	comGB	1397790..1398797 (-)	1008	WP_251925657.1	type II secretion system F family protein	Machinery gene
A4W82_RS07255 (A4W82_07155)	comGA	1398790..1399680 (-)	891	WP_251925659.1	competence type IV pilus ATPase ComGA	Machinery gene
A4W82_RS07260 (A4W82_07160)	-	1399795..1400526 (-)	732	WP_011375002.1	YebC/PmpR family DNA-binding transcriptional regulator	-
A4W82_RS07265 (A4W82_07165)	-	1400617..1401117 (-)	501	WP_016265379.1	VanZ family protein	-
A4W82_RS07270 (A4W82_07170)	-	1401214..1401588 (+)	375	WP_011375004.1	hypothetical protein	-

Sequence

Protein

• Length: 247 a.a.
• Molecular weight: 28537.34 Da
• Isoelectric Point: 10.4860

>NTDB_id=179994 A4W82_RS07240 WP_251925655.1 1396779..1397522(-) (comGD) [Latilactobacillus sakei strain TMW 1.46]
MKAARLSKNEGFTLIEMCVVLAIVSLLSWLPIYQIKQYRAQQAEQLFLHQFETSWDAARQYVAIEPRAVRVMWDAPTHTI
TFKGAGEAFRNHQLVLPETLTVSNPAEWHLINITHNKGIKPRTLKLKSTLNHREYQYKVQMMWGYYMFRSRPAFLLVENI
IALTLVLGACWLLTVSLLHFKQQQTLKQQQVAQQAVLAMAAEQLRAHQTVKKRWQMGRTIYTVTANQQKLKVTTKAGESV
AINWTTD

Nucleotide

• Length: 744 bp

>NTDB_id=179994 A4W82_RS07240 WP_251925655.1 1396779..1397522(-) (comGD) [Latilactobacillus sakei strain TMW 1.46]
TTGAAGGCGGCACGGTTAAGCAAAAATGAAGGTTTTACGTTGATTGAAATGTGCGTGGTCTTAGCGATTGTGAGCTTATT
AAGCTGGTTGCCGATTTATCAGATTAAGCAATATCGTGCGCAACAGGCTGAGCAGTTGTTTCTACATCAATTTGAAACGA
GTTGGGATGCAGCTCGTCAATATGTGGCAATTGAACCGCGTGCCGTCCGTGTGATGTGGGATGCACCAACACATACGATC
ACATTTAAAGGTGCTGGTGAGGCGTTTAGAAATCACCAACTTGTATTACCAGAAACTTTAACAGTGAGTAATCCAGCCGA
ATGGCATTTAATTAATATTACTCATAATAAGGGTATCAAACCGCGCACCTTAAAGTTAAAATCAACGCTTAATCATCGTG
AATATCAGTATAAAGTCCAGATGATGTGGGGGTATTACATGTTCAGAAGTAGGCCGGCTTTTTTACTCGTTGAAAATATC
ATCGCCTTAACCTTAGTATTAGGGGCTTGCTGGCTATTAACGGTAAGTCTACTACACTTTAAGCAACAACAAACGCTTAA
ACAACAGCAAGTTGCACAACAGGCGGTTCTAGCAATGGCCGCTGAGCAGTTGAGAGCGCATCAGACAGTGAAAAAACGCT
GGCAAATGGGGCGAACAATCTATACGGTGACGGCTAATCAGCAGAAATTAAAGGTAACCACAAAGGCAGGTGAGTCGGTT
GCGATTAATTGGACGACCGATTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (7-28)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGD	Latilactobacillus sakei subsp. sakei 23K	97.917	58.3	0.571
comGE	Latilactobacillus sakei subsp. sakei 23K	99.01	40.891	0.405