Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AMD27_RS01455 Genome accession   NZ_CP015110
Coordinates   325361..326587 (-) Length   408 a.a.
NCBI ID   WP_067655373.1    Uniprot ID   A0A145WPJ5
Organism   Acinetobacter sp. TGL-Y2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 320361..331587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMD27_RS01425 (AMD27_01425) - 321400..321588 (+) 189 WP_067655357.1 hypothetical protein -
  AMD27_RS01430 (AMD27_01430) - 321659..321982 (+) 324 WP_067655359.1 pyrimidine/purine nucleoside phosphorylase -
  AMD27_RS01435 (AMD27_01435) rlmB 322125..322874 (+) 750 WP_067655362.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AMD27_RS01440 (AMD27_01440) - 322945..323868 (+) 924 WP_067655364.1 DMT family transporter -
  AMD27_RS01445 (AMD27_01445) coaE 323865..324476 (-) 612 WP_067655367.1 dephospho-CoA kinase -
  AMD27_RS01450 (AMD27_01450) pilD 324501..325361 (-) 861 WP_067655370.1 prepilin peptidase Machinery gene
  AMD27_RS01455 (AMD27_01455) pilC 325361..326587 (-) 1227 WP_067655373.1 type II secretion system F family protein Machinery gene
  AMD27_RS01460 (AMD27_01460) pilB 326613..328322 (-) 1710 WP_067655376.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AMD27_RS01465 (AMD27_01465) tpiA 328587..329378 (+) 792 WP_067655379.1 triose-phosphate isomerase -
  AMD27_RS01470 (AMD27_01470) secG 329392..329721 (+) 330 WP_067655382.1 preprotein translocase subunit SecG -
  AMD27_RS01500 (AMD27_01500) rimP 330757..331284 (+) 528 WP_067655385.1 ribosome maturation factor RimP -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44937.40 Da        Isoelectric Point: 9.9542

>NTDB_id=176699 AMD27_RS01455 WP_067655373.1 325361..326587(-) (pilC) [Acinetobacter sp. TGL-Y2]
MAVKKAQMMPTFAYDGVDRKGTKVKGELPARNMQLAKVTLRKQGITIKSIREKRKNILEGLMKKKVSTLDITIFTRQLAT
MMKAGVPLVQSFEIVAEGLDNPSMREVVLGIKGEVEGGNTFAGALKKYPQHFDQLFCSLVESGEQSGALETMLDRVAIYK
EKSELLKQKIKKAMKYPMAVIVVALIVTVILMVKVVPVFSEMFASFGAELPAFTQMVVGMSNWTQKYWFVLLIVIIAVIF
AFLEAKKRSKKFRDTLDKMALKAPIFGDLVYKAIIARYSRTLSTTFAAGVPLIDALQSTAGATNNVVYEEAVMRIRDDVS
TGQQLQFAMRATNLFPSMAIQMVAIGEESGALDAMLDKVATHFENEVDNAVDGLTSMMEPLIMAVLGVLVGGLVIAMYLP
IFQMGSVV

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=176699 AMD27_RS01455 WP_067655373.1 325361..326587(-) (pilC) [Acinetobacter sp. TGL-Y2]
ATGGCGGTCAAAAAAGCGCAAATGATGCCAACATTTGCATATGATGGGGTTGATCGAAAAGGCACAAAAGTTAAAGGGGA
GCTGCCTGCACGTAATATGCAGTTGGCAAAGGTCACTTTGCGTAAGCAAGGCATCACCATCAAATCCATTCGAGAAAAAC
GTAAAAATATTCTCGAAGGTTTAATGAAGAAAAAAGTTTCGACTTTAGATATCACGATTTTCACCCGCCAGTTGGCGACC
ATGATGAAAGCAGGTGTGCCTTTGGTGCAAAGCTTTGAAATTGTGGCTGAAGGTTTAGATAACCCTAGCATGCGTGAAGT
CGTCTTGGGGATTAAAGGTGAAGTCGAAGGGGGGAATACTTTCGCAGGTGCCTTGAAAAAATATCCACAGCATTTTGACC
AATTGTTTTGCTCGCTGGTTGAGTCCGGCGAGCAGTCAGGTGCACTTGAGACCATGCTGGACCGCGTGGCCATCTATAAA
GAAAAAAGTGAGCTGTTAAAACAAAAAATTAAGAAAGCCATGAAATATCCAATGGCTGTCATTGTGGTCGCTTTGATTGT
GACCGTTATTTTGATGGTCAAAGTGGTGCCTGTGTTTTCGGAAATGTTCGCCTCTTTCGGCGCAGAACTTCCCGCTTTCA
CCCAAATGGTGGTGGGTATGTCCAATTGGACACAAAAATACTGGTTTGTTTTACTGATTGTGATTATTGCGGTGATTTTC
GCTTTTTTAGAAGCCAAAAAACGCAGTAAAAAATTCCGTGACACTTTGGATAAAATGGCACTCAAAGCACCTATTTTTGG
GGATTTGGTTTATAAAGCGATTATTGCTCGTTATAGCCGCACATTGTCGACCACCTTTGCAGCAGGTGTACCGCTGATCG
ATGCATTGCAATCCACTGCTGGTGCAACCAACAACGTGGTCTATGAAGAAGCCGTGATGCGTATTCGAGACGATGTCTCT
ACTGGTCAGCAGCTTCAATTTGCTATGCGTGCCACCAACCTATTTCCTTCTATGGCGATACAAATGGTGGCGATTGGTGA
AGAGTCAGGTGCACTCGATGCCATGCTAGATAAAGTCGCAACCCACTTTGAAAATGAAGTCGACAATGCCGTAGATGGCT
TAACCTCCATGATGGAGCCTTTAATTATGGCTGTGCTTGGGGTACTCGTCGGTGGCTTGGTGATTGCCATGTACCTTCCT
ATTTTCCAGATGGGCTCCGTGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(74-197)

Type II secretion system (T2SS), protein F (T2SSF)

(279-400)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A145WPJ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Acinetobacter baumannii D1279779

86.765

100

0.868
  pilC Acinetobacter baylyi ADP1

80.637

100

0.806
  pilC Pseudomonas stutzeri DSM 10701

60.988

99.265

0.605
  pilC Legionella pneumophila strain ERS1305867

51.852

99.265

0.515
  pilG Neisseria gonorrhoeae MS11

47.236

97.549

0.461
  pilG Neisseria meningitidis 44/76-A

47.236

97.549

0.461
  pilC Vibrio cholerae strain A1552

43.382

100

0.434
  pilC Vibrio campbellii strain DS40M4

40.199

98.775

0.397
  pilC Thermus thermophilus HB27

36.908

98.284

0.363

Multiple sequence alignment