Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AMD27_RS01460 Genome accession   NZ_CP015110
Coordinates   326613..328322 (-) Length   569 a.a.
NCBI ID   WP_067655376.1    Uniprot ID   A0A145WPK5
Organism   Acinetobacter sp. TGL-Y2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 321613..333322
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMD27_RS01430 (AMD27_01430) - 321659..321982 (+) 324 WP_067655359.1 pyrimidine/purine nucleoside phosphorylase -
  AMD27_RS01435 (AMD27_01435) rlmB 322125..322874 (+) 750 WP_067655362.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AMD27_RS01440 (AMD27_01440) - 322945..323868 (+) 924 WP_067655364.1 DMT family transporter -
  AMD27_RS01445 (AMD27_01445) coaE 323865..324476 (-) 612 WP_067655367.1 dephospho-CoA kinase -
  AMD27_RS01450 (AMD27_01450) pilD 324501..325361 (-) 861 WP_067655370.1 prepilin peptidase Machinery gene
  AMD27_RS01455 (AMD27_01455) pilC 325361..326587 (-) 1227 WP_067655373.1 type II secretion system F family protein Machinery gene
  AMD27_RS01460 (AMD27_01460) pilB 326613..328322 (-) 1710 WP_067655376.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AMD27_RS01465 (AMD27_01465) tpiA 328587..329378 (+) 792 WP_067655379.1 triose-phosphate isomerase -
  AMD27_RS01470 (AMD27_01470) secG 329392..329721 (+) 330 WP_067655382.1 preprotein translocase subunit SecG -
  AMD27_RS01500 (AMD27_01500) rimP 330757..331284 (+) 528 WP_067655385.1 ribosome maturation factor RimP -
  AMD27_RS01505 (AMD27_01505) nusA 331324..332808 (+) 1485 WP_067655388.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63097.45 Da        Isoelectric Point: 5.3236

>NTDB_id=176700 AMD27_RS01460 WP_067655376.1 326613..328322(-) (pilB) [Acinetobacter sp. TGL-Y2]
MTLEQSVPKFSGFNRRLVDEGVLTAQQMQTALDSSKKNKQKLIPYLIEHLNIKPLKIAELVAQEFGEPLFDLDSFNPELL
PKDITDANLINKHLVVPIFQRGSSLFVATSDPTNMEALDAIRFNTKLNISSVIVEHHKLEKFIEKNFAEADDFNFGDEDL
DVDVIDTDNSKTEDDNEPSGDDSPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIANPPLQLANRLASR
LKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNALPTLFGEKLVLRILDASSAMLGIDALGYEPAQKALFMDALEKPQ
GMLLITGPTGSGKTVSLYTGLNILNTETANISTAEDPVEINLEGINQVNVNTKVGLTFSAALKSFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCHHCKAPVDIPKQSLFEMGFT
EVDLADPDLQIYKPVGCTECRDGYKGRIGIYEVMKVTPEISKIIMEDGNALEIAAASEKSGFNNLRRSGLMKVISGLTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=176700 AMD27_RS01460 WP_067655376.1 326613..328322(-) (pilB) [Acinetobacter sp. TGL-Y2]
ATGACTTTAGAGCAAAGTGTACCAAAATTTTCGGGGTTTAATCGTCGCTTGGTCGACGAAGGTGTTTTGACTGCCCAACA
AATGCAAACTGCTTTGGATAGTTCTAAGAAAAATAAACAAAAACTTATTCCGTATCTCATTGAACATCTTAATATTAAAC
CACTTAAAATTGCTGAATTGGTGGCGCAAGAGTTTGGTGAACCTTTATTTGACCTAGATTCATTCAATCCTGAGTTATTG
CCTAAAGACATTACTGATGCCAATTTAATCAATAAGCATTTGGTTGTGCCTATTTTTCAACGTGGCAGCTCACTGTTCGT
TGCGACCAGCGACCCGACCAACATGGAAGCGCTGGATGCGATTCGCTTCAACACCAAACTTAATATTTCGTCCGTAATTG
TTGAACATCACAAATTAGAAAAATTTATTGAGAAAAATTTTGCCGAAGCAGATGACTTTAATTTTGGTGATGAAGACCTA
GACGTTGATGTCATCGACACAGATAACTCCAAGACTGAAGATGATAATGAGCCTTCTGGTGATGACTCTCCGATTGTAAA
ATACATCAATAAGTTGTTGGTCGATGCGATTCGCATGGGTGCATCAGATTTACACTTTGAACCTTATGAAAAGTCCTATC
GGGTTCGTTATCGTGTCGATGGCGTTTTACGTCATATTGCCAATCCTCCCTTACAACTGGCGAACCGCTTAGCCTCACGT
TTAAAAGTCATGTCACAAATGGACATTTCTGAAAAGCGCGTACCCCAAGATGGTCGTATTAAGCTTAAGCTGTCCAAAAC
CAAAGCCATTGATTTCCGTGTGAACGCCCTACCTACTCTGTTTGGTGAAAAACTGGTTTTACGTATCCTAGACGCATCCA
GTGCCATGCTGGGTATTGATGCGCTGGGGTATGAGCCTGCTCAAAAAGCCCTGTTTATGGATGCTTTAGAGAAACCACAA
GGCATGCTACTGATTACAGGTCCGACAGGTTCAGGTAAAACCGTATCGCTTTATACCGGTCTCAATATTTTAAATACAGA
AACGGCTAACATTTCAACCGCCGAAGACCCAGTCGAAATTAACTTGGAAGGGATTAATCAGGTTAACGTTAACACCAAAG
TGGGGCTAACCTTCTCTGCTGCATTGAAGTCTTTTTTACGTCAAGATCCAGACATTATTATGGTCGGTGAGATTCGAGAC
CTAGAGACCGCTGAAATTGCCATTAAAGCGGCGCAAACCGGTCATATGGTGATGTCAACACTGCATACCAACAGTGCACC
AGAAACATTGACCCGTTTACGTAATATGGGCGTACCTTCTTTTAATATCGCAACCTCCGTCAATTTGGTGATTGCACAGC
GTTTAGCACGTCGCCTATGTCATCACTGTAAAGCACCCGTAGATATTCCCAAACAAAGCTTATTTGAAATGGGTTTTACT
GAAGTAGATTTAGCCGATCCTGATTTACAAATTTACAAGCCGGTGGGCTGTACAGAATGCCGTGACGGCTATAAAGGTCG
TATCGGAATTTACGAAGTCATGAAAGTGACCCCTGAAATTTCTAAGATTATTATGGAAGATGGCAATGCACTTGAAATTG
CAGCAGCCTCTGAAAAATCGGGGTTTAATAATTTAAGACGTTCTGGATTAATGAAAGTCATCAGTGGCTTAACTTCATTA
CAAGAAGTGAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-149)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A145WPK5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

77.544

100

0.777
  pilB Acinetobacter baylyi ADP1

74.956

98.946

0.742
  pilB Legionella pneumophila strain ERS1305867

50.871

100

0.513
  pilF Neisseria gonorrhoeae MS11

50.534

98.77

0.499
  pilB Vibrio parahaemolyticus RIMD 2210633

47.491

98.067

0.466
  pilB Vibrio campbellii strain DS40M4

47.559

97.188

0.462
  pilB Vibrio cholerae strain A1552

49.807

91.037

0.453
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.833

100

0.388
  pilB/pilB1 Synechocystis sp. PCC 6803

34.79

100

0.378
  pilF Thermus thermophilus HB27

36.505

100

0.371

Multiple sequence alignment