Detailed information NTG0176698

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AMD27_RS01450
• Genome accession: NZ_CP015110
• Coordinates: 324501..325361 (-)
• Length: 286 a.a.
• NCBI ID: WP_067655370.1
• Uniprot ID: A0A145WPI9
• Organism: Acinetobacter sp. TGL-Y2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 319501..330361

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AMD27_RS01425 (AMD27_01425)	-	321400..321588 (+)	189	WP_067655357.1	hypothetical protein	-
AMD27_RS01430 (AMD27_01430)	-	321659..321982 (+)	324	WP_067655359.1	pyrimidine/purine nucleoside phosphorylase	-
AMD27_RS01435 (AMD27_01435)	rlmB	322125..322874 (+)	750	WP_067655362.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
AMD27_RS01440 (AMD27_01440)	-	322945..323868 (+)	924	WP_067655364.1	DMT family transporter	-
AMD27_RS01445 (AMD27_01445)	coaE	323865..324476 (-)	612	WP_067655367.1	dephospho-CoA kinase	-
AMD27_RS01450 (AMD27_01450)	pilD	324501..325361 (-)	861	WP_067655370.1	prepilin peptidase	Machinery gene
AMD27_RS01455 (AMD27_01455)	pilC	325361..326587 (-)	1227	WP_067655373.1	type II secretion system F family protein	Machinery gene
AMD27_RS01460 (AMD27_01460)	pilB	326613..328322 (-)	1710	WP_067655376.1	type IV-A pilus assembly ATPase PilB	Machinery gene
AMD27_RS01465 (AMD27_01465)	tpiA	328587..329378 (+)	792	WP_067655379.1	triose-phosphate isomerase	-
AMD27_RS01470 (AMD27_01470)	secG	329392..329721 (+)	330	WP_067655382.1	preprotein translocase subunit SecG	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 31988.43 Da
• Isoelectric Point: 8.2618

>NTDB_id=176698 AMD27_RS01450 WP_067655370.1 324501..325361(-) (pilD) [Acinetobacter sp. TGL-Y2]
MNEFIVYLIQNPLALYLTIGILSLCVGSFLNVVIYRTPKMMEQEWHQECQMLLHPEQPIIDESKLSLSKPASTCPKCKTP
IRWYQNIPVVSWLVLRGKCGACQNPISMRYPLIELLTTICSLTVVAVYGPTIQMLFGLVLTWVLIALTFIDFDTQLLPDR
YTLTLAALGLGVNSYAIYTSPTSAIWGYLIGFLCLWIVYYLFKVVTGKEGMGYGDFKLLAALGAWMGPFLLPLIVLLSSL
VGAIIGIILLKIKKQNQPFAFGPYIAIAGWIAFLWGEQIMKIYLGG

Nucleotide

• Length: 861 bp

>NTDB_id=176698 AMD27_RS01450 WP_067655370.1 324501..325361(-) (pilD) [Acinetobacter sp. TGL-Y2]
ATGAACGAATTTATTGTATATCTGATTCAAAACCCTCTCGCGCTGTATCTCACGATCGGGATTCTGAGTTTATGTGTGGG
GAGTTTTCTCAATGTGGTGATCTATCGCACACCTAAAATGATGGAGCAAGAGTGGCATCAAGAATGCCAAATGCTGCTTC
ATCCTGAACAACCGATCATTGATGAAAGTAAGCTAAGTCTGAGCAAGCCGGCATCGACCTGCCCAAAATGCAAAACTCCC
ATTCGTTGGTACCAAAATATTCCGGTGGTGAGCTGGCTGGTGCTCCGTGGAAAATGTGGCGCATGCCAAAATCCGATTAG
TATGCGCTACCCCTTGATTGAACTCTTGACGACCATCTGCTCACTCACTGTAGTGGCTGTCTATGGTCCTACCATTCAAA
TGCTGTTTGGCTTGGTCTTAACTTGGGTACTGATTGCACTAACCTTTATCGACTTTGATACACAGCTGTTGCCTGACCGT
TATACCTTAACCTTGGCTGCTTTGGGCTTAGGCGTGAACAGTTACGCGATTTATACCTCACCCACATCTGCAATTTGGGG
TTATCTCATCGGTTTCTTATGTCTTTGGATTGTCTACTATTTATTTAAAGTGGTCACAGGCAAAGAAGGTATGGGTTATG
GAGACTTTAAGCTGTTAGCCGCCTTAGGCGCTTGGATGGGCCCTTTTCTGTTGCCTTTAATTGTTTTACTCTCATCATTG
GTCGGTGCCATTATCGGCATCATTCTCCTCAAAATCAAAAAGCAAAATCAACCCTTCGCCTTTGGTCCTTATATTGCCAT
TGCGGGTTGGATTGCTTTTTTATGGGGAGAGCAAATCATGAAAATTTATTTGGGTGGATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-126)

• Type IV leader peptidase family (Peptidase_A24) (138-247)

3D structure

• AlphaFold DB: A0A145WPI9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	81.469	100	0.815
pilD	Acinetobacter nosocomialis M2	81.469	100	0.815
pilD	Vibrio campbellii strain DS40M4	53.903	94.056	0.507
pilD	Vibrio cholerae strain A1552	54.104	93.706	0.507
pilD	Neisseria gonorrhoeae MS11	47.273	96.154	0.455