Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AXW93_RS24845 Genome accession   NZ_CP014866
Coordinates   5228243..5229460 (+) Length   405 a.a.
NCBI ID   WP_003107299.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PA_154197     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5223243..5234460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXW93_RS24820 (AXW93_24040) nadC 5223344..5224192 (+) 849 WP_080911812.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AXW93_RS24830 (AXW93_24050) - 5224373..5225686 (-) 1314 WP_231296149.1 O-antigen ligase family protein -
  AXW93_RS24835 (AXW93_24055) - 5225844..5226308 (-) 465 WP_043103599.1 pilin -
  AXW93_RS24840 (AXW93_24060) pilB 5226539..5228239 (+) 1701 WP_058161256.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AXW93_RS24845 (AXW93_24065) pilC 5228243..5229460 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  AXW93_RS24850 (AXW93_24070) pilD 5229461..5230333 (+) 873 WP_080911813.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  AXW93_RS24855 (AXW93_24075) coaE 5230330..5230941 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  AXW93_RS24860 (AXW93_24080) yacG 5230938..5231138 (+) 201 WP_019486072.1 DNA gyrase inhibitor YacG -
  AXW93_RS24865 (AXW93_24085) - 5231175..5231384 (-) 210 WP_003094660.1 hypothetical protein -
  AXW93_RS24870 (AXW93_24090) - 5231490..5232179 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  AXW93_RS24875 (AXW93_24095) - 5232176..5232646 (-) 471 WP_019486073.1 hypothetical protein -
  AXW93_RS24880 (AXW93_24100) - 5232643..5233068 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  AXW93_RS24885 (AXW93_24105) - 5233201..5233830 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  AXW93_RS24890 (AXW93_24110) - 5233827..5234276 (+) 450 WP_003161439.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44001.86 Da        Isoelectric Point: 9.8103

>NTDB_id=174855 AXW93_RS24845 WP_003107299.1 5228243..5229460(+) (pilC) [Pseudomonas aeruginosa strain PA_154197]
MADKALKTSVFVWEGTDKKGTKVKGELSSQNPTLVKAQLRKQGITPVKVRKKGISLLGAGKKIKPMDIALFTRQMSTMMA
AGVPLLQSFDIISEGFDNPNMRKLVEEIKQEVAGGNSLANSLRKKPQYFDSLYCNLVDAGEQSGALETLLDRVATYKEKT
EALKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFQSVFEGFGAELPAFTMMVINISNVLQEWWLLVLLMMGGAGFLLN
HAYKRSEKFRDATDRTVLKLPIVGAILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRDAVGKIKQDVSTGM
QLNFSMRTTNIFPSMAIQMTAIGEESGALDDMLAKVAGFYEQEVDNAVDNLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=174855 AXW93_RS24845 WP_003107299.1 5228243..5229460(+) (pilC) [Pseudomonas aeruginosa strain PA_154197]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCGTATGGGAAGGCACTGACAAGAAAGGCACCAAGGTCAAGGGCGAGCT
GTCCAGCCAAAACCCGACCCTGGTCAAGGCGCAACTGCGCAAGCAGGGCATCACGCCGGTCAAGGTCCGCAAGAAAGGCA
TCTCCCTGCTGGGGGCGGGGAAGAAGATCAAGCCTATGGACATCGCCCTGTTCACCCGCCAGATGTCCACCATGATGGCC
GCCGGCGTGCCACTGCTGCAGTCGTTCGACATCATCAGCGAAGGCTTCGACAATCCGAACATGCGCAAGCTGGTGGAGGA
GATCAAGCAGGAAGTCGCCGGCGGCAACAGCCTGGCCAACTCGCTGCGCAAGAAGCCGCAGTACTTCGACTCGCTCTACT
GCAACCTGGTGGACGCCGGCGAACAATCCGGCGCCCTGGAAACCCTGCTCGACCGGGTCGCCACCTACAAGGAAAAGACC
GAGGCGCTGAAGGCCAAGATCAAGAAGGCCATGACCTACCCGATCGCGGTGATCGTCGTCGCCATCATCGTTTCGGCGAT
CCTACTGATCAAGGTGGTGCCGCAGTTCCAGTCGGTGTTCGAGGGCTTCGGCGCCGAACTGCCAGCCTTCACCATGATGG
TCATCAACATTTCCAACGTACTCCAGGAATGGTGGCTCCTGGTACTGCTGATGATGGGCGGCGCCGGCTTCCTGCTGAAC
CACGCCTACAAGCGTTCGGAGAAGTTCCGCGACGCCACCGACCGCACGGTACTGAAGCTGCCGATCGTCGGCGCCATCCT
CTACAAATCCGCCGTGGCACGCTACGCGCGGACGCTGTCGACCACCTTCGCCGCCGGCGTGCCATTGGTGGAAGCCCTCG
ACTCGGTTTCCGGCGCCACCGGCAACGTGGTGTTCAGGGACGCGGTGGGCAAGATCAAGCAGGACGTGTCCACCGGCATG
CAGCTGAACTTCTCCATGCGCACCACCAACATCTTCCCCAGCATGGCCATCCAGATGACCGCCATCGGCGAGGAATCGGG
CGCGCTGGACGACATGCTGGCCAAGGTCGCTGGCTTCTACGAGCAGGAAGTGGATAATGCCGTGGACAACCTGACCGCCC
TGATGGAACCGATGATCATGGCGGTCCTCGGGGTCCTGGTCGGTGGCCTGATCATCGCCATGTACCTGCCGATCTTCCAG
CTGGGCAACGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

78.765

100

0.788
  pilC Acinetobacter baylyi ADP1

61.029

100

0.615
  pilC Acinetobacter baumannii D1279779

60.396

99.753

0.602
  pilC Legionella pneumophila strain ERS1305867

54.293

97.778

0.531
  pilG Neisseria gonorrhoeae MS11

46.04

99.753

0.459
  pilG Neisseria meningitidis 44/76-A

45.522

99.259

0.452
  pilC Vibrio cholerae strain A1552

41.481

100

0.415
  pilC Vibrio campbellii strain DS40M4

40.648

99.012

0.402
  pilC Thermus thermophilus HB27

38.155

99.012

0.378

Multiple sequence alignment