Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AXW93_RS24840 Genome accession   NZ_CP014866
Coordinates   5226539..5228239 (+) Length   566 a.a.
NCBI ID   WP_058161256.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PA_154197     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5221539..5233239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXW93_RS24820 (AXW93_24040) nadC 5223344..5224192 (+) 849 WP_080911812.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AXW93_RS24830 (AXW93_24050) - 5224373..5225686 (-) 1314 WP_231296149.1 O-antigen ligase family protein -
  AXW93_RS24835 (AXW93_24055) - 5225844..5226308 (-) 465 WP_043103599.1 pilin -
  AXW93_RS24840 (AXW93_24060) pilB 5226539..5228239 (+) 1701 WP_058161256.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AXW93_RS24845 (AXW93_24065) pilC 5228243..5229460 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  AXW93_RS24850 (AXW93_24070) pilD 5229461..5230333 (+) 873 WP_080911813.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  AXW93_RS24855 (AXW93_24075) coaE 5230330..5230941 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  AXW93_RS24860 (AXW93_24080) yacG 5230938..5231138 (+) 201 WP_019486072.1 DNA gyrase inhibitor YacG -
  AXW93_RS24865 (AXW93_24085) - 5231175..5231384 (-) 210 WP_003094660.1 hypothetical protein -
  AXW93_RS24870 (AXW93_24090) - 5231490..5232179 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  AXW93_RS24875 (AXW93_24095) - 5232176..5232646 (-) 471 WP_019486073.1 hypothetical protein -
  AXW93_RS24880 (AXW93_24100) - 5232643..5233068 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62268.20 Da        Isoelectric Point: 5.5785

>NTDB_id=174854 AXW93_RS24840 WP_058161256.1 5226539..5228239(+) (pilB) [Pseudomonas aeruginosa strain PA_154197]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQDDGGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKNKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQAPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=174854 AXW93_RS24840 WP_058161256.1 5226539..5228239(+) (pilB) [Pseudomonas aeruginosa strain PA_154197]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAACGCGGCAACAAACTGTTCATCGGTCTCTC
CGACCCGACCAACCATCAGGCGGTCACCGATATCCAGTTCAGCACCGGCCTCACCACCGAAGCCCTGTTGGTAGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTTTACGAGGGCGCCATGGACGCCCTCGGCGACCTCGGTGATATCGATCTCGAC
GGGGTCGATGTCGGCAAGGCAGAAAGCGCCAGCCAGGACGACGGCGGCGACGATGCCGAAGATGCCCCCGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAGACCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAGGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGCATTTCCGCGCGACTC
AAGGTGATGGCCGGCCTGGATATCTCCGAACGACGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAACAA
GTCGATCGACTTCCGCGTCAATACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCCCTCGGCTACGAAGAGGATCAGAAGGAACTTTATCTGGCTGCGCTCAAACAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGCCTGAACATCCTCAATACCACCGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGCCCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAATAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAAGGTTTCCCCGAA
GAGCTGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.368

100

0.578
  pilB Acinetobacter baylyi ADP1

56.614

100

0.567
  pilB Legionella pneumophila strain ERS1305867

54.321

100

0.544
  pilB Vibrio cholerae strain A1552

51.342

98.763

0.507
  pilF Neisseria gonorrhoeae MS11

50

99.647

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

51.97

94.17

0.489
  pilB Vibrio campbellii strain DS40M4

51.032

94.17

0.481
  pilB/pilB1 Synechocystis sp. PCC 6803

37.318

100

0.408
  pilF Thermus thermophilus HB27

38.489

100

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.545

97.173

0.375

Multiple sequence alignment