Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   A3224_RS05295 Genome accession   NZ_CP014864
Coordinates   1261695..1262906 (-) Length   403 a.a.
NCBI ID   WP_067152291.1    Uniprot ID   A0A143HK76
Organism   Microbulbifer thermotolerans strain DAU221     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1256695..1267906
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3224_RS05270 (A3224_05270) argJ 1258147..1259349 (+) 1203 WP_067152283.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  A3224_RS05275 (A3224_05275) mutT 1259349..1259753 (+) 405 WP_067152285.1 8-oxo-dGTP diphosphatase MutT -
  A3224_RS05280 (A3224_05280) yacG 1259846..1260055 (-) 210 WP_067157507.1 DNA gyrase inhibitor YacG -
  A3224_RS05285 (A3224_05285) coaE 1260064..1260675 (-) 612 WP_067152287.1 dephospho-CoA kinase -
  A3224_RS05290 (A3224_05290) pilD 1260805..1261689 (-) 885 WP_067152289.1 A24 family peptidase Machinery gene
  A3224_RS05295 (A3224_05295) pilC 1261695..1262906 (-) 1212 WP_067152291.1 type II secretion system F family protein Machinery gene
  A3224_RS05300 (A3224_05300) pilB 1262976..1264688 (-) 1713 WP_067152294.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A3224_RS05305 (A3224_05305) pilA 1265146..1265574 (+) 429 WP_067157510.1 pilin Machinery gene
  A3224_RS05310 (A3224_05310) - 1265685..1267562 (+) 1878 WP_067152297.1 hypothetical protein -

Sequence

Protein


Download         Length: 403 a.a.        Molecular weight: 43534.30 Da        Isoelectric Point: 9.2880

>NTDB_id=174815 A3224_RS05295 WP_067152291.1 1261695..1262906(-) (pilC) [Microbulbifer thermotolerans strain DAU221]
MANAAAVTYVYKGVDSKGNKVEGEINGTSPALIKAQLRKQGIIANRVQKKPKPLFGGKKKVKPEDIALFTRQMATMMKAG
VPLVQSFEIVADGLDNQGVKELIFKIRDDVASGTAFADALRKHPLYFDDLFCNLVASGEQSGALETMLDRIATYKEKTES
LKKKIKKAMTYPIAVIVVAIVVTSILLIKVVPQFAETFSSFGADLPAFTQMVVGMSEWMQANWFIALICAVIGIGGTLEA
KKRNKKVADFFDRLILKMPILGQITYNSIAARFARTLSTTFAAGVPLIDALKSVAGATGNVIYEDATLKIRDSVATGIPL
NGAMRASNLYPTMLVQMAAIGEESGALDEMLGKAADFYEEAVDNMVDNLTTLLEPMIMAVLGILVGGLMVAMYLPIFQLG
QVV

Nucleotide


Download         Length: 1212 bp        

>NTDB_id=174815 A3224_RS05295 WP_067152291.1 1261695..1262906(-) (pilC) [Microbulbifer thermotolerans strain DAU221]
ATGGCCAACGCCGCAGCGGTCACCTACGTCTACAAGGGTGTGGACAGTAAAGGCAACAAGGTCGAGGGAGAGATCAACGG
CACCAGCCCTGCGCTGATCAAGGCGCAGCTGCGCAAGCAGGGCATTATAGCCAACCGGGTGCAGAAAAAGCCCAAGCCCT
TATTTGGCGGCAAGAAAAAAGTCAAACCTGAAGATATCGCGCTGTTCACACGGCAGATGGCCACCATGATGAAGGCCGGG
GTGCCACTGGTACAGAGCTTCGAAATCGTCGCCGACGGCCTGGACAATCAGGGCGTTAAGGAACTGATTTTTAAAATCCG
CGACGACGTGGCCTCCGGTACCGCCTTTGCCGATGCCCTGCGCAAGCACCCGCTCTATTTCGACGATCTCTTCTGCAACC
TGGTGGCCTCCGGTGAGCAGTCCGGCGCCCTGGAAACCATGCTGGACCGCATCGCCACCTATAAAGAAAAAACCGAATCC
CTGAAGAAAAAAATCAAAAAGGCGATGACCTACCCGATTGCGGTAATCGTTGTCGCCATTGTGGTTACCTCTATTCTGCT
GATCAAGGTAGTACCCCAGTTCGCCGAAACCTTCTCCAGCTTTGGCGCCGACCTGCCGGCATTTACCCAAATGGTAGTGG
GTATGTCTGAGTGGATGCAGGCCAACTGGTTTATTGCCCTGATTTGTGCAGTAATCGGGATCGGGGGTACCCTTGAAGCC
AAAAAGCGCAACAAAAAAGTGGCCGATTTCTTTGACCGGTTAATCCTCAAAATGCCAATTCTCGGCCAGATTACCTATAA
CTCCATCGCCGCCCGTTTCGCCCGTACGCTGTCCACCACTTTCGCCGCGGGTGTTCCCTTGATCGATGCCCTAAAGTCCG
TTGCTGGCGCCACCGGCAATGTGATTTATGAGGACGCCACCCTTAAAATCCGCGATTCTGTGGCCACCGGTATTCCGCTC
AACGGCGCCATGCGCGCTTCCAACCTCTACCCCACCATGCTGGTACAGATGGCCGCGATTGGTGAAGAGTCTGGCGCCCT
GGACGAAATGCTGGGCAAGGCCGCGGACTTTTACGAGGAGGCCGTGGACAATATGGTGGATAATCTGACAACCCTGCTGG
AACCGATGATTATGGCGGTGCTGGGTATTCTGGTCGGCGGCCTGATGGTGGCTATGTATCTGCCGATCTTCCAACTGGGT
CAGGTTGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(69-192)

Type II secretion system (T2SS), protein F (T2SSF)

(273-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A143HK76

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Acinetobacter baumannii D1279779

62.814

98.759

0.62
  pilC Pseudomonas stutzeri DSM 10701

63.291

98.015

0.62
  pilC Acinetobacter baylyi ADP1

62.469

98.511

0.615
  pilC Legionella pneumophila strain ERS1305867

57.75

99.256

0.573
  pilG Neisseria gonorrhoeae MS11

45.455

98.263

0.447
  pilG Neisseria meningitidis 44/76-A

45.202

98.263

0.444
  pilC Vibrio cholerae strain A1552

41.667

98.263

0.409
  pilC Vibrio campbellii strain DS40M4

40.704

98.759

0.402

Multiple sequence alignment