Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A3224_RS05300 Genome accession   NZ_CP014864
Coordinates   1262976..1264688 (-) Length   570 a.a.
NCBI ID   WP_067152294.1    Uniprot ID   A0A143HK11
Organism   Microbulbifer thermotolerans strain DAU221     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1257976..1269688
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3224_RS05270 (A3224_05270) argJ 1258147..1259349 (+) 1203 WP_067152283.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  A3224_RS05275 (A3224_05275) mutT 1259349..1259753 (+) 405 WP_067152285.1 8-oxo-dGTP diphosphatase MutT -
  A3224_RS05280 (A3224_05280) yacG 1259846..1260055 (-) 210 WP_067157507.1 DNA gyrase inhibitor YacG -
  A3224_RS05285 (A3224_05285) coaE 1260064..1260675 (-) 612 WP_067152287.1 dephospho-CoA kinase -
  A3224_RS05290 (A3224_05290) pilD 1260805..1261689 (-) 885 WP_067152289.1 A24 family peptidase Machinery gene
  A3224_RS05295 (A3224_05295) pilC 1261695..1262906 (-) 1212 WP_067152291.1 type II secretion system F family protein Machinery gene
  A3224_RS05300 (A3224_05300) pilB 1262976..1264688 (-) 1713 WP_067152294.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A3224_RS05305 (A3224_05305) pilA 1265146..1265574 (+) 429 WP_067157510.1 pilin Machinery gene
  A3224_RS05310 (A3224_05310) - 1265685..1267562 (+) 1878 WP_067152297.1 hypothetical protein -
  A3224_RS05315 (A3224_05315) - 1267691..1268632 (+) 942 WP_197463058.1 decaprenyl-phosphate phosphoribosyltransferase -
  A3224_RS05320 (A3224_05320) - 1268632..1269588 (+) 957 WP_067152299.1 lysylphosphatidylglycerol synthase transmembrane domain-containing protein -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62366.13 Da        Isoelectric Point: 5.1595

>NTDB_id=174816 A3224_RS05300 WP_067152294.1 1262976..1264688(-) (pilB) [Microbulbifer thermotolerans strain DAU221]
MSVSQLSGLAKRLVADQVLDEATAVSATKAAKRENQTFAQHAVEAKLVKSRDLAGIASQAFGSPLFDLSSYNFDLIPRDI
VDEKLISKHFALPLYKRGNRLFVAVADPTNLAGLDEINFNTGLNTEAVLVEADKLAKAIDSYLSDGGDMGAGLDGMDDED
LDSLDVDGADDERNDDDSLGGDEAPVVRFVNKVLLDAIRTGASDIHFEPYEKSYRVRFRTDGVLHEVAKPPIQLATRLAA
RLKVMSKMDISERRVPQDGRIKMKLSKTKAIDFRVNSLPTLWGEKIVLRILDPSSAKLGIDALGYEDDQKKLYLDALAQP
QGMILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPVEINLEGINQVNVSAKVGLNFAEALRSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPSFNIATSVSLIIAQRLARRLCSECKKPIDLPQEVLKQEGF
DAVTIDRKEWTIYQPVGCEHCSKGYKGRVGVYEVVRITEGISRIIMEGGNSIQIADQARKEGFNNLRTSALRKVVMGITS
LEEANRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=174816 A3224_RS05300 WP_067152294.1 1262976..1264688(-) (pilB) [Microbulbifer thermotolerans strain DAU221]
ATGAGTGTTAGCCAACTCTCGGGCCTGGCCAAGCGTTTGGTAGCCGACCAGGTTCTCGATGAAGCCACCGCGGTTTCCGC
CACCAAGGCGGCCAAGCGGGAAAATCAGACCTTTGCCCAGCACGCGGTGGAGGCCAAACTGGTTAAGAGCCGGGATCTTG
CCGGTATCGCCTCGCAGGCTTTTGGCTCACCGCTGTTCGATCTCTCAAGCTATAACTTTGATCTGATCCCCAGGGATATT
GTTGACGAAAAACTGATCAGCAAACACTTTGCCCTACCCCTTTACAAGCGCGGAAACCGCTTGTTTGTAGCCGTCGCCGA
TCCCACCAATCTCGCCGGGCTGGACGAGATCAATTTCAATACAGGCCTCAATACTGAAGCGGTACTGGTCGAAGCGGACA
AACTGGCCAAGGCCATCGACAGCTACCTGTCCGATGGCGGCGATATGGGGGCCGGCCTCGACGGTATGGATGACGAGGAT
CTGGATTCCCTAGATGTCGATGGTGCTGACGACGAGCGCAATGATGACGACAGCCTGGGAGGCGACGAAGCACCGGTTGT
GCGTTTTGTTAACAAGGTGCTGCTAGACGCAATCCGCACTGGTGCCTCGGATATCCACTTCGAACCCTACGAAAAAAGCT
ACCGGGTGCGCTTCCGTACCGACGGGGTGCTGCACGAGGTGGCCAAACCGCCGATCCAGCTGGCAACTCGCCTGGCCGCG
CGTCTAAAGGTGATGTCCAAGATGGATATCTCCGAACGGCGGGTGCCCCAAGACGGCCGTATTAAGATGAAGCTGTCCAA
AACCAAAGCTATTGACTTCCGGGTTAACAGCCTGCCGACGCTTTGGGGAGAAAAAATCGTTCTGCGGATTCTCGATCCCT
CCTCCGCAAAACTCGGTATCGACGCCCTCGGTTACGAGGACGACCAGAAAAAGCTCTATCTGGACGCGCTGGCCCAGCCC
CAGGGCATGATTCTGGTGACCGGTCCCACCGGCTCCGGCAAAACGGTATCCCTGTATACGGGTCTCAATATCCTCAATAC
ACCCGAGCGGAATATCTCCACGGCGGAAGACCCGGTGGAAATCAATCTGGAGGGCATCAACCAGGTCAACGTCTCTGCCA
AGGTGGGATTGAACTTTGCCGAGGCGCTGCGCTCCTTCCTCCGCCAGGACCCGGATATCGTTATGGTGGGGGAGATCCGT
GACCTGGAAACCGCGGAAATCGCAATCAAGGCCGCGCAGACGGGCCACTTGGTGCTCTCCACCCTGCACACCAATTCGGC
ACCGGAAACCCTGACGCGCTTGATGAATATGGGGGTTCCCTCTTTCAATATCGCAACCTCCGTCAGTCTCATCATCGCCC
AGCGTCTGGCGCGACGGCTGTGCAGCGAGTGCAAGAAGCCTATCGACCTCCCACAAGAAGTACTGAAGCAAGAGGGGTTC
GACGCGGTCACCATCGACCGCAAGGAGTGGACCATCTACCAGCCCGTGGGCTGCGAGCACTGCAGCAAGGGCTATAAGGG
GCGCGTGGGGGTCTATGAAGTGGTTCGCATCACCGAGGGGATCTCCAGAATTATAATGGAGGGCGGCAACTCTATTCAGA
TTGCCGACCAGGCGCGCAAGGAAGGCTTTAACAATCTGCGCACCTCCGCATTACGCAAGGTGGTAATGGGAATTACCAGT
CTGGAGGAGGCAAACCGGGTAACCAAAGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A143HK11

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

62.081

99.474

0.618
  pilB Acinetobacter baylyi ADP1

60.141

99.474

0.598
  pilB Legionella pneumophila strain ERS1305867

55.712

99.825

0.556
  pilB Vibrio cholerae strain A1552

53.119

98.421

0.523
  pilB Vibrio parahaemolyticus RIMD 2210633

55.854

91.404

0.511
  pilB Vibrio campbellii strain DS40M4

54.735

92.632

0.507
  pilF Neisseria gonorrhoeae MS11

49.476

100

0.496
  pilB/pilB1 Synechocystis sp. PCC 6803

37.561

100

0.405

Multiple sequence alignment