Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   A3224_RS05290 Genome accession   NZ_CP014864
Coordinates   1260805..1261689 (-) Length   294 a.a.
NCBI ID   WP_067152289.1    Uniprot ID   A0A143HLD4
Organism   Microbulbifer thermotolerans strain DAU221     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1255805..1266689
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3224_RS05270 (A3224_05270) argJ 1258147..1259349 (+) 1203 WP_067152283.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  A3224_RS05275 (A3224_05275) mutT 1259349..1259753 (+) 405 WP_067152285.1 8-oxo-dGTP diphosphatase MutT -
  A3224_RS05280 (A3224_05280) yacG 1259846..1260055 (-) 210 WP_067157507.1 DNA gyrase inhibitor YacG -
  A3224_RS05285 (A3224_05285) coaE 1260064..1260675 (-) 612 WP_067152287.1 dephospho-CoA kinase -
  A3224_RS05290 (A3224_05290) pilD 1260805..1261689 (-) 885 WP_067152289.1 A24 family peptidase Machinery gene
  A3224_RS05295 (A3224_05295) pilC 1261695..1262906 (-) 1212 WP_067152291.1 type II secretion system F family protein Machinery gene
  A3224_RS05300 (A3224_05300) pilB 1262976..1264688 (-) 1713 WP_067152294.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A3224_RS05305 (A3224_05305) pilA 1265146..1265574 (+) 429 WP_067157510.1 pilin Machinery gene

Sequence

Protein


Download         Length: 294 a.a.        Molecular weight: 32170.05 Da        Isoelectric Point: 7.0177

>NTDB_id=174814 A3224_RS05290 WP_067152289.1 1260805..1261689(-) (pilD) [Microbulbifer thermotolerans strain DAU221]
MLETLVSHQALLLGSAFVLGLLVGSFLNVVIYRLPIMMEREYKRDFHSYFGTNPDPAEQKILDEPFNLILPYSHCPSCGA
EVKPWHNVPLISFLLLKGRCSHCGTPISKRYPLVELVTGLLTALVVWQLGFTWQALAGCGFTWALVALTGIDFDKQLLPD
SITLPLLWTGLLVNLWGLFTPLSDAVIGAMAGYLSLWAVFHLFKLVTGKEGMGAGDFKILAAIGAWFGWQMLPMVILLSA
GVGALAGLSWSLIAGRDKNLPIAFGPYLAGAGWIAMLWGERIVNWYLNFSGLGS

Nucleotide


Download         Length: 885 bp        

>NTDB_id=174814 A3224_RS05290 WP_067152289.1 1260805..1261689(-) (pilD) [Microbulbifer thermotolerans strain DAU221]
ATGCTAGAAACACTGGTTTCTCACCAAGCGCTGCTCCTCGGCAGCGCTTTTGTTTTGGGCCTATTAGTGGGCAGCTTTCT
AAACGTGGTTATTTACCGCCTGCCCATCATGATGGAGCGGGAATACAAGCGGGATTTCCACAGCTATTTCGGCACCAATC
CCGATCCGGCAGAGCAAAAAATACTGGACGAACCCTTTAACCTTATACTGCCCTACTCCCACTGCCCCAGTTGCGGCGCC
GAGGTCAAACCTTGGCACAATGTACCGCTGATTAGCTTTCTACTCCTGAAAGGCAGGTGCAGCCATTGCGGTACCCCCAT
CTCCAAGCGCTATCCGCTGGTGGAGCTGGTTACAGGGCTGTTGACCGCGCTGGTCGTGTGGCAATTGGGCTTTACCTGGC
AGGCGCTGGCGGGCTGTGGTTTTACCTGGGCACTGGTGGCTCTAACGGGAATAGACTTCGACAAGCAACTGCTGCCGGAC
AGTATCACCCTGCCGCTGCTGTGGACGGGATTACTGGTTAATCTCTGGGGACTGTTCACTCCCCTTAGTGATGCGGTGAT
AGGGGCCATGGCTGGCTATCTCTCTCTTTGGGCTGTGTTCCACCTGTTTAAGCTGGTAACCGGCAAGGAGGGAATGGGGG
CGGGGGATTTCAAAATCCTGGCAGCCATCGGCGCCTGGTTCGGGTGGCAGATGTTGCCGATGGTCATCCTGCTGTCCGCC
GGTGTTGGCGCCCTGGCCGGATTGAGCTGGAGTCTGATCGCGGGGCGGGACAAAAACCTGCCCATCGCCTTTGGCCCCTA
TCTGGCCGGTGCCGGCTGGATAGCGATGCTGTGGGGGGAGCGGATTGTAAACTGGTATCTGAATTTCTCAGGACTCGGGT
CATAG

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(141-248)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(18-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A143HLD4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

51.812

93.878

0.486
  pilD Vibrio campbellii strain DS40M4

48.11

98.98

0.476
  pilD Acinetobacter nosocomialis M2

50.179

94.898

0.476
  pilD Acinetobacter baumannii D1279779

49.821

94.898

0.473
  pilD Neisseria gonorrhoeae MS11

48.913

93.878

0.459

Multiple sequence alignment