Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   A0U95_RS20955 Genome accession   NZ_CP014693
Coordinates   4814914..4816131 (-) Length   405 a.a.
NCBI ID   WP_069971384.1    Uniprot ID   -
Organism   Pseudomonas brassicacearum strain L13-6-12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4809914..4821131
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A0U95_RS20920 (A0U95_20880) - 4810759..4811586 (-) 828 WP_233839806.1 LysR substrate-binding domain-containing protein -
  A0U95_RS20925 (A0U95_20885) - 4811803..4812213 (+) 411 WP_003205679.1 tautomerase family protein -
  A0U95_RS20930 (A0U95_20890) - 4812255..4812941 (+) 687 WP_069971383.1 energy-coupling factor ABC transporter permease -
  A0U95_RS20935 (A0U95_20895) - 4813005..4813220 (+) 216 WP_013694234.1 hypothetical protein -
  A0U95_RS20940 (A0U95_20900) yacG 4813217..4813423 (-) 207 WP_003205675.1 DNA gyrase inhibitor YacG -
  A0U95_RS20945 (A0U95_20905) coaE 4813420..4814043 (-) 624 WP_003205673.1 dephospho-CoA kinase -
  A0U95_RS20950 (A0U95_20910) pilD 4814043..4814912 (-) 870 WP_003205672.1 prepilin peptidase Machinery gene
  A0U95_RS20955 (A0U95_20915) pilC 4814914..4816131 (-) 1218 WP_069971384.1 type II secretion system F family protein Machinery gene
  A0U95_RS20960 (A0U95_20920) pilB 4816134..4817834 (-) 1701 WP_003205669.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A0U95_RS20965 (A0U95_20925) - 4818059..4818490 (+) 432 WP_069971385.1 pilin -
  A0U95_RS20970 (A0U95_20930) - 4818820..4819173 (+) 354 WP_013694229.1 BON domain-containing protein -
  A0U95_RS20975 (A0U95_20935) - 4819502..4819798 (-) 297 WP_013694228.1 DUF2845 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44233.70 Da        Isoelectric Point: 10.0786

>NTDB_id=173303 A0U95_RS20955 WP_069971384.1 4814914..4816131(-) (pilC) [Pseudomonas brassicacearum strain L13-6-12]
MAVKAVKTDVYTWEGKDRKGTKMSGELTGQSPALVKAQLRKQGINPQKVRKKSTSIFSKGKRIKPLDIALFTRQMATMLK
AGVPLLQAFDIIGEGFDNPNXRKLVDEVKQEVAAGNSFAAALRKCPQYFDELYCNLVDAGEQAGALDTLLDRVATYKEKS
EALKAKIKKAMTYPAAVVLVAAVVTGILLVKVVPQFESVFSGFGAKLPAFTVMVIGLSEIMQQWWWVMLGGAVGAFFGVK
YALRRSERFRDWRDKWLLKLPLIGTLLYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKRAVQRIKQEVSTGM
QLNFSMRASGIFPNLAVQMTAIGEESGALDDMLDKVASFYEAEVDNLVDNLTSLMEPFIMVVLGVVVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=173303 A0U95_RS20955 WP_069971384.1 4814914..4816131(-) (pilC) [Pseudomonas brassicacearum strain L13-6-12]
ATGGCGGTCAAGGCAGTCAAAACCGATGTTTACACTTGGGAAGGCAAGGACCGCAAAGGCACGAAAATGAGTGGCGAGCT
GACGGGTCAAAGCCCGGCGCTGGTCAAGGCACAGCTGCGCAAACAAGGCATCAACCCGCAGAAGGTGCGCAAGAAATCCA
CCTCGATATTCAGCAAGGGCAAGCGCATCAAGCCGCTGGATATCGCCCTCTTCACCCGCCAGATGGCCACCATGCTCAAG
GCTGGTGTGCCGCTGTTGCAGGCGTTCGACATCATTGGCGAAGGCTTCGACAACCCCAACANGCGCAAGCTGGTGGACGA
GGTGAAGCAGGAAGTCGCCGCTGGCAACAGCTTCGCCGCGGCGCTGCGCAAGTGCCCGCAATATTTCGACGAGCTGTACT
GCAACCTGGTAGACGCCGGCGAGCAGGCCGGTGCCCTGGACACGCTGCTGGACCGGGTCGCGACCTACAAGGAAAAGAGC
GAAGCGCTCAAGGCCAAGATCAAGAAAGCCATGACCTATCCAGCGGCCGTGGTCCTCGTCGCCGCAGTGGTCACGGGGAT
CCTGCTGGTCAAGGTCGTGCCGCAATTCGAATCGGTATTCTCCGGGTTTGGCGCGAAGCTGCCAGCCTTCACGGTGATGG
TCATAGGCCTGTCGGAAATCATGCAGCAATGGTGGTGGGTGATGCTTGGCGGGGCGGTGGGGGCTTTTTTTGGCGTGAAG
TATGCGCTCAGGCGTTCGGAGCGCTTTCGCGACTGGCGCGACAAATGGCTGCTCAAGCTGCCGCTGATCGGCACCTTGCT
GTACAAATCCGCCGTGGCCCGCTACGCCCGCACGCTGTCCACTACCTTCGCCGCCGGCGTGCCGCTGGTGGAGGCCCTGG
ATTCGGTGTCTGGCGCCACCGGCAACGTGGTATTCAAACGTGCCGTGCAGCGCATCAAGCAGGAGGTCTCGACCGGCATG
CAGTTGAATTTCTCCATGCGTGCCTCAGGGATTTTTCCGAACCTGGCGGTCCAGATGACCGCCATCGGCGAGGAATCCGG
TGCGCTGGACGACATGCTCGACAAGGTGGCGAGCTTTTATGAGGCCGAGGTGGACAATCTGGTGGACAACCTCACCAGCC
TGATGGAACCCTTCATCATGGTGGTCCTGGGGGTGGTCGTCGGTGGCCTGGTAGTTGCCATGTACCTGCCCATCTTTCAA
CTCGGCTCTGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

75.309

100

0.753
  pilC Acinetobacter baylyi ADP1

58.603

99.012

0.58
  pilC Acinetobacter baumannii D1279779

57.353

100

0.578
  pilC Legionella pneumophila strain ERS1305867

54.293

97.778

0.531
  pilC Vibrio campbellii strain DS40M4

43.939

97.778

0.43
  pilC Vibrio cholerae strain A1552

43.182

97.778

0.422
  pilG Neisseria meningitidis 44/76-A

42.222

100

0.422
  pilG Neisseria gonorrhoeae MS11

42.289

99.259

0.42
  pilC Thermus thermophilus HB27

37.406

99.012

0.37

Multiple sequence alignment