Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A0U95_RS20960 Genome accession   NZ_CP014693
Coordinates   4816134..4817834 (-) Length   566 a.a.
NCBI ID   WP_003205669.1    Uniprot ID   -
Organism   Pseudomonas brassicacearum strain L13-6-12     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4811134..4822834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A0U95_RS20925 (A0U95_20885) - 4811803..4812213 (+) 411 WP_003205679.1 tautomerase family protein -
  A0U95_RS20930 (A0U95_20890) - 4812255..4812941 (+) 687 WP_069971383.1 energy-coupling factor ABC transporter permease -
  A0U95_RS20935 (A0U95_20895) - 4813005..4813220 (+) 216 WP_013694234.1 hypothetical protein -
  A0U95_RS20940 (A0U95_20900) yacG 4813217..4813423 (-) 207 WP_003205675.1 DNA gyrase inhibitor YacG -
  A0U95_RS20945 (A0U95_20905) coaE 4813420..4814043 (-) 624 WP_003205673.1 dephospho-CoA kinase -
  A0U95_RS20950 (A0U95_20910) pilD 4814043..4814912 (-) 870 WP_003205672.1 prepilin peptidase Machinery gene
  A0U95_RS20955 (A0U95_20915) pilC 4814914..4816131 (-) 1218 WP_069971384.1 type II secretion system F family protein Machinery gene
  A0U95_RS20960 (A0U95_20920) pilB 4816134..4817834 (-) 1701 WP_003205669.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A0U95_RS20965 (A0U95_20925) - 4818059..4818490 (+) 432 WP_069971385.1 pilin -
  A0U95_RS20970 (A0U95_20930) - 4818820..4819173 (+) 354 WP_013694229.1 BON domain-containing protein -
  A0U95_RS20975 (A0U95_20935) - 4819502..4819798 (-) 297 WP_013694228.1 DUF2845 domain-containing protein -
  A0U95_RS20980 (A0U95_20940) - 4819800..4822733 (-) 2934 WP_003205661.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62754.75 Da        Isoelectric Point: 5.3203

>NTDB_id=173304 A0U95_RS20960 WP_003205669.1 4816134..4817834(-) (pilB) [Pseudomonas brassicacearum strain L13-6-12]
MNDIALSGLTKQLVLAELLTEQSAQQAYQQAQRNRIPLVSYLVQNKLVQSRQVAEIASEHFGVALLDLNSLDKETQPTGL
VSEKLVRQHHALPLWRRGNKLYVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESSSTGLEGMGDVDLDG
LDIESIDDTRQDSIAGQDTDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRVRTDGILREVARPPIQLANRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCSHCKKAIEIPEETLLKEGFPR
ERIGTFTIYEPVGCEQCNNGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKVMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=173304 A0U95_RS20960 WP_003205669.1 4816134..4817834(-) (pilB) [Pseudomonas brassicacearum strain L13-6-12]
ATGAATGACATCGCCCTCAGCGGCCTGACCAAGCAATTGGTGCTGGCCGAACTGCTCACCGAGCAAAGTGCGCAACAGGC
GTATCAACAGGCGCAACGCAATCGCATCCCCCTGGTCAGCTATCTGGTGCAGAACAAACTGGTCCAGAGCCGACAGGTCG
CGGAGATCGCCTCGGAGCATTTCGGCGTCGCCCTGCTGGACCTCAACAGCCTGGACAAAGAAACCCAACCCACCGGCCTG
GTCAGCGAAAAACTGGTGCGCCAGCATCACGCCCTGCCGCTGTGGCGGCGCGGCAACAAGCTGTACGTGGGCATCTCCGA
CCCGACCAATCACCAGGCCATCAATGACATCCAGTTCAGCACCGGCCTGACCACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGAAAAGTTTTTCGAATCCAGCAGCACGGGCCTGGAAGGCATGGGCGATGTCGACCTCGACGGC
CTGGACATCGAATCGATCGACGACACCAGGCAGGACTCCATCGCCGGCCAGGACACCGACGACGCGCCCGTGGTGCGCTT
CGTCAACAAGATGCTGCTGGACGCGATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAAATCTACCGGG
TGCGGGTGCGCACCGACGGCATACTGCGGGAAGTGGCCAGGCCGCCGATCCAGTTGGCCAACCGTATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTGGATATTTCCGAGCGGCGCAAACCCCAGGACGGGCGCCTGAAAATGCGCCTGTCGAAAACCAA
GTCCATCGATTTCCGGGTCAACACCCTGCCCACGCTCTGGGGCGAAAAAGTGGTGATCCGGATCCTCGACCCGTCCAGCG
CACAAATGGGCATCGATGCCCTGGGCTACGAACCGGACCAGAAAGACCTGTACATGGCCGCCCTCAAGCAACCGCAAGGG
CTGATCCTGGTGACTGGCCCAACCGGCTCGGGCAAGACCGTGTCGCTCTACACCGGGCTGAATATCCTCAACACCGTGGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTGAACCCGCGCCAGG
GGCTGGATTTCGCCCAGGCCCTGCGCTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGGCACCTGGTGCTCTCCACCCTGCACACCAACAGCGCCGCAGA
AACCCTGATCCGCCTGCAGAACATGGGCATTCCGGGGTTCAACATCGCCACCGCCGTGCACCTGATCATTGCCCAGCGGC
TGGCGCGCAAGCTGTGCAGTCACTGCAAGAAGGCCATCGAGATTCCCGAGGAAACCTTGCTCAAGGAAGGTTTCCCTCGG
GAACGCATCGGCACATTCACGATCTATGAGCCGGTCGGTTGCGAACAGTGCAACAACGGTTACAAAGGGCGCGTGGGGGT
TTACGAAGTGGTCAAGAACACGCCCGAGCTGCAACGGCTGATCATGGCCGAGGGCAACTCGCTGGAAATCGATCTGCAAA
TGCGCAAGGACGGTTTCAATGACCTGCGCACCTCGGGGCTGCTCAAAGTGATGCAGGGCGTCACCAGCCTCGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.304

100

0.583
  pilB Acinetobacter baumannii D1279779

57.848

100

0.58
  pilB Legionella pneumophila strain ERS1305867

55.556

100

0.557
  pilB Vibrio cholerae strain A1552

50.442

99.823

0.504
  pilB Vibrio campbellii strain DS40M4

48.596

100

0.489
  pilF Neisseria gonorrhoeae MS11

48.313

99.47

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

48.921

98.233

0.481
  pilF Thermus thermophilus HB27

36.042

100

0.36

Multiple sequence alignment