Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   VVCECT4999_RS01790 Genome accession   NZ_CP014636
Coordinates   358712..360490 (+) Length   592 a.a.
NCBI ID   WP_039449673.1    Uniprot ID   -
Organism   Vibrio vulnificus strain CECT 4999     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 353712..365490
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VVCECT4999_RS01770 (VVCECT4999_01745) pilM 356026..357033 (+) 1008 WP_017419656.1 type IV pilus assembly protein PilM Machinery gene
  VVCECT4999_RS01775 (VVCECT4999_01750) pilN 357023..357613 (+) 591 WP_017419657.1 PilN domain-containing protein Machinery gene
  VVCECT4999_RS01780 (VVCECT4999_01755) pilO 357606..358196 (+) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  VVCECT4999_RS01785 (VVCECT4999_01760) pilP 358186..358698 (+) 513 WP_038940290.1 pilus assembly protein PilP Machinery gene
  VVCECT4999_RS01790 (VVCECT4999_01765) pilQ 358712..360490 (+) 1779 WP_039449673.1 type IV pilus secretin PilQ Machinery gene
  VVCECT4999_RS01795 (VVCECT4999_01770) aroK 360692..361210 (+) 519 WP_011079357.1 shikimate kinase AroK -
  VVCECT4999_RS01800 (VVCECT4999_01775) aroB 361243..362334 (+) 1092 WP_011079358.1 3-dehydroquinate synthase -
  VVCECT4999_RS01805 (VVCECT4999_01780) - 362385..363881 (+) 1497 WP_017419661.1 AAA family ATPase -
  VVCECT4999_RS01810 (VVCECT4999_01785) - 363961..364788 (+) 828 WP_017419662.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 592 a.a.        Molecular weight: 64419.47 Da        Isoelectric Point: 4.5077

>NTDB_id=172844 VVCECT4999_RS01790 WP_039449673.1 358712..360490(+) (pilQ) [Vibrio vulnificus strain CECT 4999]
MICELMMKNKIQLFPGMLSQFVCLLSVCIFTTFANAQSEAENALKSIDFRTNAAKDAIIIVELATPATVADLKPVAEGLS
IELINTSVKDEQLYLLDVKDFSTVVESIEVFREVSKTRLVANVSGEFRHDYRLSGRYLEVTISRIKSEQEAREQSVLEKE
GKLISINFQDIPVRNVLQLIADYNGFNLVVSDSVTGNVTLRLDGVPWQQVLDIILQVKGLDKRVEGNVVLIAPQDELDLR
EKQQLEKERLAEELGSLKSEIIKVNFAKASDIAEMINGKESVSMLSERGSVAIDERTNSLLLRELPENIEVIREIINSLD
IPVKQVQIEARIVTVNEGNLDELGVRWGFTSINGSTTVGGSIENNLATIGLYNAGSGSGDSGSGGSGDEGGSNSIDDFLN
VNLAATSANASSIAFQVAKLGSDLLLDLELSALQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVSFKK
AVLSLKVTPQITPDNRLVLDLSVTQDRPGEIVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNTTDKVPLLG
DLPLLGALFRRSYENVGKRELLIFVTPKVILQ

Nucleotide


Download         Length: 1779 bp        

>NTDB_id=172844 VVCECT4999_RS01790 WP_039449673.1 358712..360490(+) (pilQ) [Vibrio vulnificus strain CECT 4999]
GTGATTTGTGAGTTGATGATGAAAAACAAAATCCAATTGTTTCCGGGAATGCTTTCCCAATTCGTCTGCCTGTTGTCTGT
TTGCATCTTTACCACTTTTGCAAACGCACAATCTGAAGCAGAAAATGCGCTCAAGAGCATCGATTTCAGAACCAATGCAG
CCAAAGATGCGATTATCATCGTTGAATTGGCAACGCCTGCAACCGTGGCGGATTTGAAACCAGTGGCAGAAGGATTGAGC
ATCGAGTTGATCAATACCTCGGTTAAAGATGAGCAGCTCTATCTGCTCGATGTCAAAGATTTTTCGACCGTGGTTGAAAG
TATCGAAGTATTCAGAGAAGTGTCCAAAACCCGTCTTGTTGCGAATGTCAGCGGTGAATTTCGTCACGACTATCGTCTTT
CTGGACGCTATCTCGAGGTGACAATTTCTCGCATCAAATCGGAGCAAGAAGCCCGTGAACAAAGTGTGTTGGAAAAAGAA
GGCAAGCTGATTTCTATCAACTTCCAAGATATTCCAGTGCGTAACGTTTTGCAGCTTATCGCAGACTACAATGGCTTCAA
CCTCGTGGTGTCTGATTCTGTCACTGGTAATGTGACGCTGAGATTGGATGGGGTACCTTGGCAGCAAGTGCTCGACATCA
TTCTTCAAGTGAAAGGTCTAGACAAACGTGTTGAAGGCAATGTTGTGCTCATTGCTCCTCAAGATGAACTGGATTTGCGA
GAGAAGCAGCAGCTTGAGAAAGAACGTCTGGCGGAAGAGCTTGGCTCCCTTAAATCGGAAATCATCAAAGTCAACTTTGC
TAAAGCGTCAGACATTGCCGAGATGATCAATGGCAAAGAGTCGGTCAGCATGCTGTCAGAGCGTGGTAGTGTGGCGATTG
ATGAACGGACAAACTCTCTTTTATTGCGTGAGTTACCCGAAAACATCGAAGTGATTCGAGAAATTATCAATTCGTTGGAT
ATCCCAGTCAAACAGGTACAAATTGAAGCACGCATTGTTACGGTGAATGAAGGCAATCTTGATGAGCTTGGTGTCCGTTG
GGGGTTCACCTCCATCAACGGCAGCACAACCGTCGGCGGTTCTATCGAAAACAACCTAGCAACCATTGGTCTCTACAATG
CAGGCAGTGGTAGTGGTGACTCAGGTAGCGGCGGCAGTGGTGATGAAGGCGGGTCGAACTCCATTGATGATTTCCTCAAT
GTCAATCTTGCTGCGACTTCTGCAAACGCCTCAAGTATTGCTTTCCAAGTGGCTAAATTGGGGTCGGATTTGTTGTTGGA
TCTCGAGCTATCAGCACTGCAGCGCGAATCAAAAGCGGAAATTATTTCTAGCCCTCGTTTGATCACAACCAATAAGAAGC
CTGCTTATATCGAGCAAGGTACAGAGATTCCTTATCTAGAGTCTTCCTCCAGTGGAGCGACCACGGTTTCCTTTAAGAAA
GCGGTGCTTAGCCTGAAAGTAACCCCTCAGATCACGCCTGACAATCGTCTCGTGTTGGATTTAAGCGTTACACAAGATAG
ACCTGGCGAAATTGTCAAAACAGGCACTGGGGAAGCGGTTGCGATTGATACTCAGCGCATCGGTACACAAGTCTTGGTCA
ATAATGGTGAAACGGTAGTGTTAGGCGGTATCTTCCAACACAGCATAACCAATACCACGGACAAGGTTCCTCTGTTAGGC
GATTTACCCTTATTGGGTGCGCTTTTCCGCCGTAGCTATGAAAACGTAGGCAAACGTGAATTGCTGATATTTGTCACACC
TAAGGTTATCTTGCAGTAG

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(186-234)

Bacterial type II and III secretion system protein (Secretin)

(432-590)

Bacterial type II/III secretion system short domain (Secretin_N)

(260-325)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

78.231

99.324

0.777
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.15

99.324

0.736
  pilQ Vibrio cholerae strain A1552

74.15

99.324

0.736
  pilQ Legionella pneumophila strain ERS1305867

41.577

94.257

0.392
  pilQ Legionella pneumophila strain Lp02

41.577

94.257

0.392
  pilQ Pseudomonas aeruginosa PAK

38.435

97.128

0.373
  comQ Acinetobacter baylyi ADP1

36.78

99.662

0.367

Multiple sequence alignment