Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   VVCECT4999_RS01775 Genome accession   NZ_CP014636
Coordinates   357023..357613 (+) Length   196 a.a.
NCBI ID   WP_017419657.1    Uniprot ID   -
Organism   Vibrio vulnificus strain CECT 4999     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 352023..362613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VVCECT4999_RS01760 (VVCECT4999_01735) oxyR 352405..353304 (+) 900 WP_026050199.1 DNA-binding transcriptional regulator OxyR -
  VVCECT4999_RS01765 (VVCECT4999_01740) - 353373..355886 (-) 2514 WP_039449670.1 penicillin-binding protein 1A -
  VVCECT4999_RS01770 (VVCECT4999_01745) pilM 356026..357033 (+) 1008 WP_017419656.1 type IV pilus assembly protein PilM Machinery gene
  VVCECT4999_RS01775 (VVCECT4999_01750) pilN 357023..357613 (+) 591 WP_017419657.1 PilN domain-containing protein Machinery gene
  VVCECT4999_RS01780 (VVCECT4999_01755) pilO 357606..358196 (+) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  VVCECT4999_RS01785 (VVCECT4999_01760) pilP 358186..358698 (+) 513 WP_038940290.1 pilus assembly protein PilP Machinery gene
  VVCECT4999_RS01790 (VVCECT4999_01765) pilQ 358712..360490 (+) 1779 WP_039449673.1 type IV pilus secretin PilQ Machinery gene
  VVCECT4999_RS01795 (VVCECT4999_01770) aroK 360692..361210 (+) 519 WP_011079357.1 shikimate kinase AroK -
  VVCECT4999_RS01800 (VVCECT4999_01775) aroB 361243..362334 (+) 1092 WP_011079358.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22644.86 Da        Isoelectric Point: 7.6441

>NTDB_id=172841 VVCECT4999_RS01775 WP_017419657.1 357023..357613(+) (pilN) [Vibrio vulnificus strain CECT 4999]
MQHKINLLPWREHKREEHKRRFVSLVALGALIAVGVQWGIGQYFSYQQRLQQERLDYLTHYIAQLDQRIQAMNIAKEEHS
QILARLKVVESLQAERNKTTAFMNLMTELIPEGVYVDKIKMNTNDVELAGISDSTARLATMLDNLERSSFLKEVEMHSIV
HDKKRFGKSFQTFTVSFVFDEHADSANTLAKEGNHG

Nucleotide


Download         Length: 591 bp        

>NTDB_id=172841 VVCECT4999_RS01775 WP_017419657.1 357023..357613(+) (pilN) [Vibrio vulnificus strain CECT 4999]
ATGCAGCATAAGATCAACCTGTTACCTTGGCGTGAACACAAGCGTGAGGAGCATAAACGTCGTTTTGTCAGTCTGGTCGC
ATTAGGTGCCTTGATCGCGGTCGGTGTTCAGTGGGGCATTGGCCAGTACTTTTCATACCAACAACGTTTGCAGCAAGAGC
GTCTCGATTATCTTACCCACTATATCGCACAGTTGGATCAGCGTATTCAAGCGATGAACATTGCCAAGGAAGAGCATAGC
CAGATTCTCGCTCGACTCAAGGTTGTCGAGTCGCTTCAAGCGGAGCGCAACAAAACCACCGCATTTATGAACTTGATGAC
GGAGCTGATCCCAGAGGGGGTGTATGTCGACAAGATCAAAATGAACACCAACGACGTTGAGTTGGCAGGTATAAGCGATA
GTACCGCTCGACTCGCCACCATGTTGGATAATTTGGAACGTTCCTCCTTCCTGAAAGAAGTGGAAATGCATTCGATTGTT
CATGACAAGAAGCGTTTTGGTAAGAGCTTCCAGACGTTTACCGTCTCTTTTGTGTTTGATGAACATGCCGATTCTGCCAA
TACGTTGGCCAAGGAGGGGAATCATGGCTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

68.508

92.347

0.633
  pilN Vibrio cholerae strain A1552

63.277

90.306

0.571

Multiple sequence alignment