Detailed information    

experimental Experimentally validated

Overview

Name   mutS   Type   Auxiliary factor
Locus tag   - Genome accession   AJ783431
Coordinates   414..2993 (+) Length   859 a.a.
NCBI ID   CAH03854.1    Uniprot ID   -
Organism   Pseudomonas stutzeri strain ATCC 17587     
Function   altered foreign DNA acquisition   
Defense system

Function

In P. stutzeri upregulation of MutS could enforce sexual isolation and downregulation could increase foreign DNA acquisition and that MutS affects mechanisms of homology-facilitated illegitimate recombination (HFIR).


Genomic Context

Location: 1..7993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Locus_0 mutS 414..2993 (+) 2580 CAH03854.1 DNA mismatch repair protein MutS Auxiliary factor
  Locus_1 fdxA 3115..3431 (+) 317 CAH03855.1 Ferredoxin I -

Sequence

Protein


Download         Length: 859 a.a.        Molecular weight: 95319.09 Da        Isoelectric Point: 5.5763

>NTDB_id=1201 CAH03854.1 414..2993(+) (mutS) [Pseudomonas stutzeri strain ATCC 17587]
MTKPNADLSAHTPMMQQYWRLKREHPDQLMFYRMGDFYELFYDDAKKAAALLDITLTARGQSAGTAIPMAGIPFHSAEGY
LARLVKLGESVVICEQIGDPAASKGPVERQVVRIITPGTVSDEALLDERRDNLLAAVVGDERLFGLSVLDIASGRFSVQE
LKGWETLLAELERLSPAELLIPDDWPQGLPLEKRRGVRRRAPWDFDRDSAFKSLCQQFSTQDLKGFGCENLTLAIGAAGC
LLAYAKETQRTALPHLRSLRHERLADTVILDGASRRNLELDVNLSGGRDNTLQSVMDRCQTAMGSRLLTRWLNRPLRNRE
ILEARQDSITCLLEHYRFEQLQPQLKDIGDLERILARIGLRNARPRDLARLRDALAALPQLQAGMQDLVAPHLLELATSI
STYPELAELLARAIIDNPPAVIRDGGVLKTGYDPELDELQSLSENAGQYLMDLETREKARTGLANLKVGYNRVHGYFIEL
PSKQAESAPADYIRRQTLKGAERFITPELKEFEDKALSAKSRALAREKLLYDELLEMLIGHLAPLQESAAALAELDMLSN
LAERALNLDLNRPRFVEQPCMRIEQGRHPVVEQVLETPFVANDLGLDDATRMLVITGPNMGGKSTYMRQTALIVLLAQIG
SFVPAAACELSLVDRIFTRIGSSDDLAGGRSTFMVEMSETANILHNASDRSLVLMDEVGRGTSTFDGLSLAWAAAEHLAK
LRAFTLFATHYFELTVLPESEPVVANVHLSATEHNERIVFLHHVLPGPASQSYGLAVAQLAGVPGEVIQRARDHLSRLET
TSLSHEAPRITPGQPAPPMQSDMFASLPHPVLEELGRINPDDVTPRQALELLYSLKTRI*

Nucleotide


Download         Length: 2580 bp        

>NTDB_id=1201 CAH03854.1 414..2993(+) (mutS) [Pseudomonas stutzeri strain ATCC 17587]
ATGACCAAGCCAAACGCCGACCTTTCCGCACATACGCCCATGATGCAGCAGTACTGGAGACTCAAGCGCGAGCATCCAGA
CCAGCTGATGTTCTATCGCATGGGCGACTTCTACGAGCTGTTCTACGACGACGCCAAGAAGGCCGCCGCGCTGCTCGACA
TCACCCTGACTGCGCGCGGTCAGTCGGCGGGCACGGCAATCCCCATGGCCGGGATTCCTTTCCATTCCGCCGAAGGCTAT
CTGGCACGACTGGTCAAGCTGGGCGAATCGGTGGTGATCTGCGAGCAGATCGGCGACCCGGCCGCCAGCAAGGGGCCGGT
GGAACGCCAGGTGGTGCGCATCATCACCCCCGGTACGGTGAGCGACGAAGCGCTACTCGATGAGCGCCGCGACAACCTGC
TGGCCGCAGTAGTCGGCGATGAGCGACTGTTCGGTCTGTCGGTGCTGGACATCGCCAGCGGACGCTTCAGCGTGCAGGAA
CTCAAGGGCTGGGAAACCCTGCTCGCCGAACTGGAGCGCCTGAGCCCGGCGGAATTGTTGATTCCCGACGACTGGCCGCA
GGGCCTGCCGCTGGAAAAGCGCCGCGGAGTGCGCCGTCGCGCGCCCTGGGATTTCGATCGCGATTCGGCATTCAAGAGCC
TCTGCCAGCAGTTCTCCACCCAGGACCTCAAGGGCTTCGGCTGCGAGAACCTGACGCTGGCCATCGGCGCCGCCGGCTGC
CTGCTCGCCTATGCCAAGGAAACCCAGCGCACCGCCCTGCCCCACCTGCGCAGCCTGCGCCACGAGCGCCTCGCCGACAC
GGTGATCCTCGACGGCGCTAGTCGGCGCAATCTGGAGCTGGACGTCAACCTTTCTGGTGGCCGCGACAACACGCTGCAAT
CGGTCATGGATCGCTGCCAGACCGCCATGGGCTCGCGGCTCCTGACCCGCTGGCTGAACCGCCCGCTGCGCAACCGCGAG
ATTCTCGAAGCGCGCCAGGATTCGATCACCTGCCTGCTCGAACACTACCGTTTCGAGCAGCTGCAGCCGCAGCTCAAGGA
CATCGGCGACCTGGAACGCATCCTCGCCCGTATCGGTCTGCGCAACGCCCGCCCGCGCGATCTGGCGCGCCTGCGCGACG
CCCTCGCCGCGTTACCGCAGCTGCAGGCCGGCATGCAGGATCTGGTGGCGCCGCATCTGCTGGAACTGGCGACAAGCATC
AGCACCTACCCGGAGTTGGCCGAACTGCTGGCCCGCGCCATCATCGACAACCCGCCGGCGGTGATCCGCGACGGCGGCGT
ACTGAAGACCGGCTACGACCCCGAGCTGGACGAGCTGCAGTCCCTCTCGGAGAACGCCGGCCAGTACCTGATGGACCTGG
AAACCCGCGAGAAGGCCCGCACCGGCCTGGCCAACCTCAAGGTCGGCTACAACCGCGTGCACGGCTACTTCATCGAGCTG
CCGAGCAAGCAGGCCGAATCCGCGCCGGCCGACTACATCCGCCGGCAGACGCTCAAGGGCGCCGAGCGCTTCATCACCCC
TGAGCTGAAGGAGTTCGAGGACAAGGCGCTGTCGGCCAAGAGCCGCGCCCTCGCCCGCGAGAAGCTGCTCTACGACGAGC
TGCTGGAAATGCTCATCGGCCACCTGGCGCCGCTGCAGGAAAGTGCCGCCGCCCTGGCCGAGCTGGACATGCTGAGCAAC
CTCGCCGAGCGCGCGCTCAATCTCGACCTGAATCGCCCGCGCTTCGTCGAGCAACCGTGCATGCGCATCGAGCAGGGTCG
CCATCCAGTCGTGGAGCAGGTATTGGAGACACCTTTCGTGGCCAACGATCTGGGTCTCGACGACGCAACCCGCATGCTGG
TGATCACCGGGCCGAACATGGGCGGTAAATCGACCTATATGCGCCAGACCGCGCTGATCGTGCTGCTGGCACAGATCGGC
AGCTTCGTCCCGGCTGCGGCTTGCGAGCTGTCGTTGGTGGATCGCATCTTCACCCGTATTGGCTCCTCGGACGATCTGGC
CGGCGGCCGCTCCACGTTCATGGTGGAAATGAGCGAAACTGCCAACATCCTGCACAACGCCAGCGATCGCAGCCTGGTGC
TGATGGACGAAGTGGGCCGCGGCACCAGTACCTTCGATGGCCTTTCGCTGGCCTGGGCTGCCGCCGAGCACCTCGCCAAA
CTGCGTGCGTTTACCCTGTTCGCCACTCATTACTTCGAGCTGACCGTATTGCCAGAAAGCGAGCCAGTGGTGGCCAACGT
CCACCTTTCTGCTACTGAGCACAACGAGCGCATCGTCTTCCTGCACCACGTACTGCCGGGGCCGGCGAGCCAGAGCTACG
GCCTGGCGGTGGCACAGCTGGCCGGCGTGCCGGGCGAAGTCATCCAGCGCGCGCGCGACCATCTGTCGCGCCTGGAAACC
ACCAGCCTGTCCCACGAAGCACCGAGAATAACGCCCGGCCAGCCTGCACCACCCATGCAGAGCGACATGTTCGCCAGCCT
GCCACATCCGGTGCTAGAGGAATTGGGGCGGATCAATCCCGATGATGTAACGCCGCGTCAGGCGCTGGAACTGCTATACA
GCTTGAAAACTCGCATTTGA

Domains

Predicted by InterproScan.

MutS domain III (MutS_III)

(274-562)

MutS domain I (MutS_I)

(12-123)

MutS family domain IV (MutS_IV)

(431-521)

MutS domain II (MutS_II)

(132-257)

MutS domain V (MutS_V)

(613-799)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Petra Meier et al. (2005) Impact of mutS inactivation on foreign DNA acquisition by natural transformation in Pseudomonas stutzeri. Journal of Bacteriology 187(1):143-54. [PMID: 15601698]