Detailed information NTG0172840

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: VVCECT4999_RS01770
• Genome accession: NZ_CP014636
• Coordinates: 356026..357033 (+)
• Length: 335 a.a.
• NCBI ID: WP_017419656.1
• Uniprot ID: A0A087I1V6
• Organism: Vibrio vulnificus strain CECT 4999
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 351026..362033

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VVCECT4999_RS01755 (VVCECT4999_01730)	-	351859..352131 (-)	273	WP_017419653.1	DUF3624 domain-containing protein	-
VVCECT4999_RS01760 (VVCECT4999_01735)	oxyR	352405..353304 (+)	900	WP_026050199.1	DNA-binding transcriptional regulator OxyR	-
VVCECT4999_RS01765 (VVCECT4999_01740)	-	353373..355886 (-)	2514	WP_039449670.1	penicillin-binding protein 1A	-
VVCECT4999_RS01770 (VVCECT4999_01745)	pilM	356026..357033 (+)	1008	WP_017419656.1	type IV pilus assembly protein PilM	Machinery gene
VVCECT4999_RS01775 (VVCECT4999_01750)	pilN	357023..357613 (+)	591	WP_017419657.1	PilN domain-containing protein	Machinery gene
VVCECT4999_RS01780 (VVCECT4999_01755)	pilO	357606..358196 (+)	591	WP_011079354.1	type 4a pilus biogenesis protein PilO	Machinery gene
VVCECT4999_RS01785 (VVCECT4999_01760)	pilP	358186..358698 (+)	513	WP_038940290.1	pilus assembly protein PilP	Machinery gene
VVCECT4999_RS01790 (VVCECT4999_01765)	pilQ	358712..360490 (+)	1779	WP_039449673.1	type IV pilus secretin PilQ	Machinery gene
VVCECT4999_RS01795 (VVCECT4999_01770)	aroK	360692..361210 (+)	519	WP_011079357.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 335 a.a.
• Molecular weight: 37654.05 Da
• Isoelectric Point: 6.3873

>NTDB_id=172840 VVCECT4999_RS01770 WP_017419656.1 356026..357033(+) (pilM) [Vibrio vulnificus strain CECT 4999]
MGKPFVTGIDIGHRSLKAVILKPYGEHYALLGYKEIIIKDGIFSDNHTLNHQEIVNSLKELKKELPLFRYQVSLAVPDSS
VISKMLSIDAELDGREREFAILQAFAHQSPFPVDELSIDYVQLVEETSKTNHSYQVYATKKEVVESRVRVAEEVGLKPVL
VDVHAHSLCNILTLAAERHPDKASYCLLDVGYDTTSLVMSRRNEPPLVKEFAYGVSHLNASIGDDLTSPDAHEKTDIFNK
ELTERVRRQLQLFQSMDSQSKVNGLWICGEGAITPLLSETLQRKLSLECEVLNPLALFEQKTAKRKRRIVDWHHFSVAAG
LAIGGIQWLEDSHAA

Nucleotide

• Length: 1008 bp

>NTDB_id=172840 VVCECT4999_RS01770 WP_017419656.1 356026..357033(+) (pilM) [Vibrio vulnificus strain CECT 4999]
ATGGGTAAACCGTTCGTTACCGGAATTGATATTGGTCATCGTAGCCTCAAGGCTGTCATTCTGAAACCTTATGGAGAGCA
CTACGCCCTGTTAGGGTACAAAGAAATAATCATTAAAGACGGTATTTTCTCAGATAACCACACTCTGAATCATCAAGAAA
TTGTCAATAGCCTGAAAGAACTGAAGAAGGAGTTGCCTCTTTTCCGTTATCAGGTGTCACTGGCGGTACCCGATAGTTCA
GTGATTAGCAAGATGTTATCGATCGATGCGGAGTTGGATGGTCGAGAACGAGAGTTCGCTATCCTTCAGGCGTTTGCACA
TCAGTCGCCATTTCCTGTCGATGAATTATCGATTGATTATGTCCAATTGGTCGAAGAAACCAGCAAAACGAATCATAGCT
ATCAAGTTTACGCCACCAAAAAAGAGGTGGTGGAAAGCCGAGTGCGTGTCGCAGAAGAAGTGGGACTGAAACCCGTGCTG
GTAGATGTACATGCGCATAGCCTGTGCAACATCTTAACGTTAGCGGCAGAGCGCCATCCAGACAAAGCCAGCTATTGCTT
GTTGGATGTGGGTTATGACACCACCTCTTTGGTGATGTCGCGTCGCAACGAACCACCTTTGGTTAAAGAATTTGCCTATG
GGGTGTCTCACCTTAATGCGTCGATAGGCGACGATCTGACTTCTCCTGATGCTCATGAGAAAACCGACATCTTTAATAAA
GAGCTGACAGAGCGAGTTCGTCGGCAATTGCAGTTGTTCCAATCCATGGATAGCCAAAGCAAAGTGAATGGCCTTTGGAT
CTGCGGTGAAGGCGCGATTACGCCGTTGTTGAGTGAAACTTTGCAGAGAAAGCTGAGTTTGGAGTGTGAAGTCCTTAATC
CTCTGGCTCTATTTGAGCAAAAAACGGCGAAAAGAAAGCGCCGTATTGTTGATTGGCACCATTTTTCGGTCGCCGCGGGA
TTAGCGATTGGTGGCATTCAGTGGCTGGAGGATAGCCATGCAGCATAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-323)

3D structure

• AlphaFold DB: A0A087I1V6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio campbellii strain DS40M4	53.134	100	0.531
pilM	Vibrio cholerae strain A1552	48.974	100	0.499