Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AXG94_RS10965 Genome accession   NZ_CP014262
Coordinates   2584339..2585556 (+) Length   405 a.a.
NCBI ID   WP_024779581.1    Uniprot ID   A0A3M3EEL4
Organism   Pseudomonas corrugata strain RM1-1-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2579339..2590556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXG94_RS10945 (AXG94_10935) - 2580769..2581065 (+) 297 WP_053192127.1 DUF2845 domain-containing protein -
  AXG94_RS10950 (AXG94_10940) - 2581369..2581722 (-) 354 WP_024779584.1 BON domain-containing protein -
  AXG94_RS10955 (AXG94_10945) - 2581995..2582411 (-) 417 WP_055135086.1 pilin -
  AXG94_RS10960 (AXG94_10950) pilB 2582636..2584336 (+) 1701 WP_053192129.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AXG94_RS10965 (AXG94_10955) pilC 2584339..2585556 (+) 1218 WP_024779581.1 type II secretion system F family protein Machinery gene
  AXG94_RS10970 (AXG94_10960) pilD 2585558..2586427 (+) 870 WP_055135083.1 prepilin peptidase Machinery gene
  AXG94_RS10975 (AXG94_10965) coaE 2586438..2587061 (+) 624 WP_055135082.1 dephospho-CoA kinase -
  AXG94_RS10980 (AXG94_10970) yacG 2587058..2587264 (+) 207 WP_024779578.1 DNA gyrase inhibitor YacG -
  AXG94_RS10985 (AXG94_10975) - 2587269..2587484 (-) 216 WP_024779577.1 hypothetical protein -
  AXG94_RS10990 (AXG94_10980) - 2587548..2588234 (-) 687 WP_024779576.1 energy-coupling factor ABC transporter permease -
  AXG94_RS10995 (AXG94_10985) - 2588569..2589195 (+) 627 WP_024779575.1 DUF1780 domain-containing protein -
  AXG94_RS11000 (AXG94_10990) - 2589294..2589467 (+) 174 WP_003185543.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43958.55 Da        Isoelectric Point: 10.0139

>NTDB_id=169932 AXG94_RS10965 WP_024779581.1 2584339..2585556(+) (pilC) [Pseudomonas corrugata strain RM1-1-4]
MAAKAVKTDTYTWEGKDRKGAKMSGELTGQSPALVKAQLRKQGINPGKVRKKSTSIFSKGKRIKPLDIALFTRQMATMLK
AGVPLLQAFDIIGEGFDNANMRKLVDEVKQEVAAGNSFAASLRKCPQYFDELYCNLVDAGEQAGALDTLLDRVATYKEKS
EALKAKIKKAMTYPAAVVLVAAVVTGILLVKVVPQFESVFSGFGAKLPAFTLMVIGLSEFMQESWWLLLGGAVGTFFGVK
YALKRSQGFRDWRDKWLLKLPLVGTLMYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKRAVQRIRQDVSTGM
QLNFSMRASGIFPNLAIQMTAIGEESGALDDMLDKVAGFYEAEVDNLVDNLTSLMEPFIMVVLGVVVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=169932 AXG94_RS10965 WP_024779581.1 2584339..2585556(+) (pilC) [Pseudomonas corrugata strain RM1-1-4]
ATGGCGGCCAAGGCAGTAAAAACCGATACCTATACCTGGGAAGGCAAAGACCGCAAGGGCGCGAAGATGTCCGGCGAGCT
GACCGGCCAGAGCCCGGCCCTGGTCAAGGCGCAATTGCGCAAGCAAGGCATCAACCCGGGCAAGGTCCGCAAGAAATCCA
CCTCGATATTCAGCAAGGGCAAACGCATCAAGCCGCTGGATATCGCCCTCTTCACCCGCCAGATGGCGACGATGCTCAAG
GCCGGTGTGCCCCTGCTGCAAGCCTTCGACATCATTGGCGAAGGCTTCGACAATGCCAACATGCGCAAGCTGGTAGACGA
GGTGAAGCAGGAGGTCGCCGCCGGCAACAGTTTCGCCGCGTCGTTGCGCAAGTGCCCGCAATACTTCGACGAGTTGTACT
GCAACCTGGTGGACGCGGGTGAACAGGCCGGTGCCCTGGACACCTTGCTGGATCGGGTCGCGACCTACAAGGAGAAGAGC
GAAGCCCTCAAGGCCAAGATCAAGAAAGCCATGACCTATCCGGCGGCCGTCGTGCTCGTAGCGGCAGTCGTCACCGGCAT
TCTACTGGTCAAGGTGGTGCCGCAGTTCGAATCGGTGTTCTCCGGATTCGGCGCGAAACTGCCTGCGTTCACCCTGATGG
TCATCGGCCTGTCGGAGTTCATGCAAGAGTCGTGGTGGCTGCTGCTGGGCGGGGCTGTGGGCACATTTTTTGGCGTGAAG
TATGCGCTCAAGCGCTCCCAGGGTTTTCGTGACTGGCGTGATAAATGGCTGCTCAAGCTGCCGCTGGTGGGCACCCTGAT
GTACAAATCCGCGGTAGCCCGCTACGCCCGGACACTGTCCACCACCTTCGCCGCCGGCGTGCCACTGGTGGAGGCTCTGG
ACTCTGTCTCCGGCGCCACCGGCAACGTGGTGTTCAAACGTGCGGTCCAGCGCATCCGGCAAGATGTCTCGACCGGTATG
CAGTTGAATTTTTCCATGCGTGCCTCAGGCATATTCCCGAACCTGGCGATCCAGATGACCGCCATCGGCGAGGAATCCGG
AGCACTGGACGATATGCTCGACAAGGTGGCAGGTTTCTATGAGGCCGAGGTCGACAACCTGGTGGACAATCTCACCAGTC
TGATGGAACCGTTCATCATGGTGGTCCTGGGGGTGGTTGTCGGCGGTCTTGTGGTTGCCATGTACCTGCCCATCTTTCAA
CTCGGCTCTGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3M3EEL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.321

100

0.743
  pilC Acinetobacter baylyi ADP1

58.881

100

0.598
  pilC Acinetobacter baumannii D1279779

58.416

99.753

0.583
  pilC Legionella pneumophila strain ERS1305867

54.66

98.025

0.536
  pilC Vibrio campbellii strain DS40M4

43.735

100

0.44
  pilG Neisseria gonorrhoeae MS11

42.716

100

0.427
  pilC Vibrio cholerae strain A1552

42.469

100

0.425
  pilG Neisseria meningitidis 44/76-A

41.975

100

0.42
  pilC Thermus thermophilus HB27

38.5

98.765

0.38

Multiple sequence alignment