Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   AWB63_RS09135 Genome accession   NZ_CP014144
Coordinates   1982655..1983596 (-) Length   313 a.a.
NCBI ID   WP_038676863.1    Uniprot ID   A0AB37CKN4
Organism   Streptococcus salivarius strain JF     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1977655..1988596
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWB63_RS09095 (AWB63_09095) - 1977730..1978923 (-) 1194 WP_002885831.1 acetate kinase -
  AWB63_RS09100 (AWB63_09100) comYH 1978980..1979936 (-) 957 WP_038676858.1 class I SAM-dependent methyltransferase Machinery gene
  AWB63_RS09105 (AWB63_09105) comGG 1979981..1980298 (-) 318 WP_002887013.1 competence type IV pilus minor pilin ComGG -
  AWB63_RS09110 (AWB63_09110) comYF 1980276..1980713 (-) 438 WP_002887014.1 competence type IV pilus minor pilin ComGF Machinery gene
  AWB63_RS09115 (AWB63_09115) comGE 1980700..1980930 (-) 231 WP_002887015.1 competence type IV pilus minor pilin ComGE -
  AWB63_RS09120 (AWB63_09120) comYD 1980962..1981390 (-) 429 WP_254593949.1 competence type IV pilus minor pilin ComGD Machinery gene
  AWB63_RS09125 (AWB63_09125) comYC 1981350..1981664 (-) 315 WP_170314557.1 competence type IV pilus major pilin ComGC Machinery gene
  AWB63_RS09130 (AWB63_09130) comYB 1981673..1982773 (-) 1101 WP_126405090.1 competence type IV pilus assembly protein ComGB Machinery gene
  AWB63_RS09135 (AWB63_09135) comGA/cglA/cilD 1982655..1983596 (-) 942 WP_038676863.1 competence type IV pilus ATPase ComGA Machinery gene
  AWB63_RS09140 (AWB63_09140) - 1983676..1984038 (-) 363 WP_002887019.1 DUF1033 family protein -
  AWB63_RS09145 (AWB63_09145) rpoC 1984201..1987839 (-) 3639 WP_002887020.1 DNA-directed RNA polymerase subunit beta' -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35241.32 Da        Isoelectric Point: 5.9272

>NTDB_id=168767 AWB63_RS09135 WP_038676863.1 1982655..1983596(-) (comGA/cglA/cilD) [Streptococcus salivarius strain JF]
MVTEFAKEMIKNADSCGAQDIYVIPRQDNYELHMRVGQERRLIDVYRPDFMASLIGHFKFVAGMMVGEKRRSQLGSCDYD
CGDGQKVSLRLSTVGDYRGLESLVIRVLHSERRELVYWNQGIQPIMDALDYRGLYLFAGPVGSGKTTLMHELVQERFKGQ
QVISIEDPVEIKQDNVLQLQVNQAIDMTYDNLIKLSLRHRPDVLIIGEIRDKETARAVIRASLTGVTVLSTIHAKSVAGV
YERLLDLGVDKSELDNALQGIAYMRLIKGGGVIDFASENFQSHSSISWNQQLEGLVKQGYLTEGDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=168767 AWB63_RS09135 WP_038676863.1 1982655..1983596(-) (comGA/cglA/cilD) [Streptococcus salivarius strain JF]
ATGGTAACAGAATTTGCTAAGGAAATGATCAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTATGTCATTCCACGTCA
GGATAATTACGAGCTCCATATGCGAGTTGGCCAGGAGAGGAGATTAATTGATGTATATCGGCCTGATTTCATGGCTAGTC
TTATTGGTCACTTTAAATTTGTGGCGGGAATGATGGTGGGTGAGAAGCGTAGGAGTCAACTAGGTTCCTGTGACTATGAT
TGTGGTGATGGTCAAAAGGTTTCTCTGCGTTTGTCTACCGTTGGGGATTATCGCGGCTTAGAAAGTTTAGTTATTCGTGT
TCTACATTCCGAACGTCGAGAATTAGTGTACTGGAATCAAGGAATTCAGCCTATTATGGATGCTTTGGATTATCGTGGCT
TATATCTCTTTGCAGGTCCCGTTGGTTCTGGAAAGACTACGCTTATGCATGAGTTGGTTCAGGAGCGTTTTAAGGGGCAG
CAGGTGATTTCGATTGAGGATCCTGTGGAAATTAAACAGGATAATGTTTTGCAACTTCAGGTGAATCAAGCAATTGACAT
GACCTATGATAATTTGATTAAGCTATCACTGCGTCACCGCCCGGATGTTTTGATTATTGGAGAAATTCGAGATAAGGAGA
CTGCTCGAGCGGTTATTAGAGCTAGTCTGACGGGAGTGACTGTTCTTTCAACTATTCACGCAAAGAGTGTGGCAGGTGTT
TATGAGCGTCTTCTGGACCTTGGTGTAGATAAGTCTGAGTTGGATAATGCCCTTCAAGGGATTGCCTACATGCGTCTGAT
TAAGGGAGGAGGTGTGATTGATTTTGCCAGTGAAAATTTCCAAAGCCATTCGTCAATCAGCTGGAACCAGCAGTTGGAAG
GCTTGGTTAAACAAGGATATCTCACTGAAGGGGATATCCAAGGGGAAAAAATTAAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.916

99.361

0.655
  comYA Streptococcus mutans UA159

64.856

100

0.649
  comYA Streptococcus mutans UA140

64.856

100

0.649
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

64.952

99.361

0.645
  comGA/cglA/cilD Streptococcus pneumoniae R6

64.952

99.361

0.645
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

64.952

99.361

0.645
  comGA/cglA/cilD Streptococcus pneumoniae D39

64.952

99.361

0.645
  comYA Streptococcus gordonii str. Challis substr. CH1

62.3

100

0.623
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.577

99.681

0.604
  comGA Lactococcus lactis subsp. cremoris KW2

51.125

99.361

0.508
  comGA Latilactobacillus sakei subsp. sakei 23K

41.697

86.581

0.361

Multiple sequence alignment