Detailed information NTG0168763

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: AWB63_RS09110
• Genome accession: NZ_CP014144
• Coordinates: 1980276..1980713 (-)
• Length: 145 a.a.
• NCBI ID: WP_002887014.1
• Uniprot ID: A0A1V0GF29
• Organism: Streptococcus salivarius strain JF
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1975276..1985713

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AWB63_RS09075 (AWB63_09075)	-	1975319..1975981 (-)	663	WP_002887009.1	CPBP family intramembrane glutamic endopeptidase	-
AWB63_RS09080 (AWB63_09080)	-	1976094..1976540 (-)	447	WP_037605837.1	hypothetical protein	-
AWB63_RS09085 (AWB63_09085)	-	1976614..1977270 (-)	657	WP_002887011.1	CPBP family intramembrane glutamic endopeptidase	-
AWB63_RS09090 (AWB63_09090)	-	1977282..1977479 (-)	198	WP_002885716.1	helix-turn-helix transcriptional regulator	-
AWB63_RS09095 (AWB63_09095)	-	1977730..1978923 (-)	1194	WP_002885831.1	acetate kinase	-
AWB63_RS09100 (AWB63_09100)	comYH	1978980..1979936 (-)	957	WP_038676858.1	class I SAM-dependent methyltransferase	Machinery gene
AWB63_RS09105 (AWB63_09105)	comGG	1979981..1980298 (-)	318	WP_002887013.1	competence type IV pilus minor pilin ComGG	-
AWB63_RS09110 (AWB63_09110)	comYF	1980276..1980713 (-)	438	WP_002887014.1	competence type IV pilus minor pilin ComGF	Machinery gene
AWB63_RS09115 (AWB63_09115)	comGE	1980700..1980930 (-)	231	WP_002887015.1	competence type IV pilus minor pilin ComGE	-
AWB63_RS09120 (AWB63_09120)	comYD	1980962..1981390 (-)	429	WP_254593949.1	competence type IV pilus minor pilin ComGD	Machinery gene
AWB63_RS09125 (AWB63_09125)	comYC	1981350..1981664 (-)	315	WP_170314557.1	competence type IV pilus major pilin ComGC	Machinery gene
AWB63_RS09130 (AWB63_09130)	comYB	1981673..1982773 (-)	1101	WP_126405090.1	competence type IV pilus assembly protein ComGB	Machinery gene
AWB63_RS09135 (AWB63_09135)	comGA/cglA/cilD	1982655..1983596 (-)	942	WP_038676863.1	competence type IV pilus ATPase ComGA	Machinery gene
AWB63_RS09140 (AWB63_09140)	-	1983676..1984038 (-)	363	WP_002887019.1	DUF1033 family protein	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16155.57 Da
• Isoelectric Point: 8.4658

>NTDB_id=168763 AWB63_RS09110 WP_002887014.1 1980276..1980713(-) (comYF) [Streptococcus salivarius strain JF]
MSLNSKVRAFTLLECLVALLVIAGSVQVYQGLTTVLVSNVKQVKQQESQDWLLFVQQMEAELEGCQLVKVEGNKLYVKQD
NQDLAFGLSSATDFRKTNADGRGYQPMLFNVKASTISQNNQIVTIQVTLKNGLQRSFVYAFEKTG

Nucleotide

• Length: 438 bp

>NTDB_id=168763 AWB63_RS09110 WP_002887014.1 1980276..1980713(-) (comYF) [Streptococcus salivarius strain JF]
ATGTCTCTCAATAGCAAGGTTCGTGCTTTTACCCTCTTGGAATGTCTAGTGGCCTTGTTGGTGATTGCTGGTTCAGTTCA
AGTTTATCAAGGTCTGACCACAGTATTGGTGAGCAATGTTAAGCAGGTCAAGCAACAAGAAAGCCAAGACTGGCTTCTAT
TCGTTCAACAAATGGAGGCAGAATTAGAAGGTTGTCAGTTGGTAAAGGTAGAGGGGAACAAACTCTATGTCAAGCAGGAT
AATCAAGACTTAGCTTTTGGACTATCAAGTGCTACTGATTTTCGAAAAACAAATGCTGACGGTCGAGGCTACCAACCCAT
GCTTTTTAATGTAAAAGCTAGTACAATTAGCCAAAATAACCAGATAGTCACCATCCAAGTGACTCTTAAAAATGGGCTAC
AAAGGAGTTTTGTTTATGCTTTTGAAAAAACAGGTTAG

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (39-134)

3D structure

• AlphaFold DB: A0A1V0GF29

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	58.156	97.241	0.566
comYF	Streptococcus mutans UA159	57.447	97.241	0.559
comGF/cglF	Streptococcus pneumoniae Rx1	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae D39	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae R6	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae TIGR4	50	97.931	0.49
comGF/cglF	Streptococcus mitis SK321	47.222	99.31	0.469
comGF/cglF	Streptococcus mitis NCTC 12261	47.222	99.31	0.469
comGF	Lactococcus lactis subsp. cremoris KW2	45.324	95.862	0.434