Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AL468_RS20135 Genome accession   NZ_CP014134
Coordinates   2278108..2279856 (+) Length   582 a.a.
NCBI ID   WP_006742777.1    Uniprot ID   A0AA92LSF7
Organism   Vibrio diabolicus strain FDAARGOS_99     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2273108..2284856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL468_RS20115 (AL468_11530) pilM 2275416..2276432 (+) 1017 WP_005395718.1 type IV pilus assembly protein PilM Machinery gene
  AL468_RS20120 (AL468_11535) pilN 2276416..2276991 (+) 576 WP_046874634.1 PilN domain-containing protein Machinery gene
  AL468_RS20125 (AL468_11540) pilO 2276984..2277577 (+) 594 WP_012841351.1 type 4a pilus biogenesis protein PilO Machinery gene
  AL468_RS20130 (AL468_11545) pilP 2277567..2278082 (+) 516 WP_005395715.1 pilus assembly protein PilP Machinery gene
  AL468_RS20135 (AL468_11550) pilQ 2278108..2279856 (+) 1749 WP_006742777.1 type IV pilus secretin PilQ family protein Machinery gene
  AL468_RS20140 (AL468_11555) aroK 2280044..2280562 (+) 519 WP_005381319.1 shikimate kinase AroK -
  AL468_RS20145 (AL468_11560) aroB 2280595..2281695 (+) 1101 WP_012841354.1 3-dehydroquinate synthase -
  AL468_RS20150 (AL468_11565) - 2281748..2283262 (+) 1515 WP_104974737.1 AAA family ATPase -
  AL468_RS20155 (AL468_11570) - 2283332..2284171 (+) 840 WP_047009939.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63760.73 Da        Isoelectric Point: 4.4533

>NTDB_id=168578 AL468_RS20135 WP_006742777.1 2278108..2279856(+) (pilQ) [Vibrio diabolicus strain FDAARGOS_99]
MKMRQGLTRTPRILTGLFSLWLFVFLAPLVAAEEVSSNALKSIDFRTNKDKDAVIVVELASPAAIVDVKRVQEGLSIDLL
NTSVKDEQLYLLDVKDFSTVVESVEVFRDTSTTRLVAHINEEYTHDYRLTGRFLEIKVAKIKPNEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQQLEQTMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGIRWGFTSINGNNTVGGSIENNLATIGLYNGGGEGEDGGDGDGVGIDDFLNVNLAATSPNA
TSIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVTFKKAVLSLKVTPQ
ITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFR
RSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=168578 AL468_RS20135 WP_006742777.1 2278108..2279856(+) (pilQ) [Vibrio diabolicus strain FDAARGOS_99]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATACTAACTGGGCTCTTCTCCTTATGGTTGTTTGTATTTTTAGC
CCCTTTAGTGGCAGCAGAAGAAGTATCGTCTAATGCCTTAAAGAGTATCGATTTTCGCACTAATAAAGATAAAGACGCCG
TTATTGTCGTTGAACTGGCGTCACCTGCCGCGATTGTTGATGTCAAACGCGTACAGGAAGGGTTAAGTATCGATTTGCTT
AATACTTCGGTAAAAGACGAGCAACTGTATCTCCTAGACGTAAAAGATTTCTCGACTGTGGTTGAAAGCGTAGAGGTGTT
TCGCGATACCTCAACTACGCGCTTGGTTGCGCACATTAATGAAGAATATACGCATGATTACCGTTTAACGGGGCGATTCT
TGGAAATCAAAGTGGCTAAGATCAAACCCAATGAGAAAGCCCCGGATAAAAGCATTCTAGAAAAAGAAGGCAAGCTCATT
TCGATTAACTTCCAAGATATTCCTGTACGTAACGTGTTACAGCTGATTGCTGACTACAACCAGTTCAACTTGGTTGTCTC
AGACTCCGTAGAAGGTAACCTCACACTTCGTCTTGATGGTGTTCCGTGGCAGCAAGTGTTGGATATTATTTTGCAAGTTA
AGGGGTTAGATAAGCGTGTTGATGGCAACGTGATTCTGGTTGCTCCAACAGATGAGCTTGATCTGCGCGAAAAACAGCAA
CTCGAAAAGCAGCAGCTAGAACAAACGATGGGAGAGCTTTCGTCTGAAATCATCAAGGTAAACTTCGCTAAAGCATCAGA
TATTGCAGAGATGATCAACGGAGAAGGGAACATCAGTATGTTGTCTGAGCGAGGCAGTATGACCATTGATGAGCGGACTA
ACTCGTTATTGATTCGTGAGCTACCTGAAAACATTGAAGTGATTCGAGAAATTATTGAATCTTTGGATATCCCGGTGAAG
CAGGTTCAAATTGAAGCGCGTATTGTGACCATCAATGAAGGCAACATGGACGAGTTGGGAATTCGCTGGGGCTTTACCTC
TATTAATGGCAATAACACGGTTGGTGGTTCTATCGAAAACAACCTTGCCACGATCGGTTTATATAACGGTGGTGGTGAAG
GAGAAGACGGTGGTGACGGTGATGGAGTAGGTATCGATGACTTCCTAAACGTAAACTTAGCTGCAACCAGTCCAAACGCG
ACCAGCATTGCTTTTCAGGTTGCAAAATTAGGCTCAGACACCTTGTTAGACCTCGAGCTTTCTGCGCTGCAACAAGAGTC
CAAAGCTGAGATTATCTCAAGCCCACGTTTGATCACTACCAATAAGAAGCCTGCCTATATTGAGCAGGGTACCGAGATAC
CTTATCTAGAGTCCTCATCAAGTGGTGCTACGACGGTAACGTTCAAAAAAGCAGTATTGAGTTTGAAGGTGACGCCACAA
ATTACACCGGATAACCGCCTTGTGTTGGACTTAAGCGTTACGCAAGATAGACCTGGACAAGTCGTGAAAACAGGTACTGG
TGAAGCCGTTGCGATTGATACTCAACGTATCGGGACGCAAGTGTTAGTAAATAACGGTGAAACCGTCGTTCTTGGTGGTA
TTTTCCAGCACAGCATTACTAACTCGGTGGACAAAGTGCCTTTGTTAGGCGATCTGCCACTATTAGGTGCGCTTTTCCGT
CGAAGTTATGAAAATGTTGGCAAAAGTGAGCTGTTGATTTTCGTCACCCCTAAGGTGGTGATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(422-580)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

92.955

100

0.93
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.443

100

0.746
  pilQ Vibrio cholerae strain A1552

74.443

100

0.746
  pilQ Legionella pneumophila strain Lp02

40.787

96.048

0.392
  pilQ Legionella pneumophila strain ERS1305867

40.787

96.048

0.392
  pilQ Pseudomonas aeruginosa PAK

39.716

96.907

0.385
  comQ Acinetobacter baylyi ADP1

37.349

99.828

0.373
  pilQ Acinetobacter baumannii strain A118

36.409

100

0.373
  pilQ Acinetobacter baumannii D1279779

36.409

100

0.373

Multiple sequence alignment