Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AL468_RS20115 Genome accession   NZ_CP014134
Coordinates   2275416..2276432 (+) Length   338 a.a.
NCBI ID   WP_005395718.1    Uniprot ID   A0AAE4S9C7
Organism   Vibrio diabolicus strain FDAARGOS_99     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2270416..2281432
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL468_RS20095 (AL468_11510) - 2270849..2271577 (-) 729 WP_104974735.1 redoxin family protein -
  AL468_RS20105 (AL468_11520) oxyR 2271748..2272659 (+) 912 WP_005395720.1 DNA-binding transcriptional regulator OxyR -
  AL468_RS20110 (AL468_11525) - 2272711..2275272 (-) 2562 WP_104974736.1 PBP1A family penicillin-binding protein -
  AL468_RS20115 (AL468_11530) pilM 2275416..2276432 (+) 1017 WP_005395718.1 type IV pilus assembly protein PilM Machinery gene
  AL468_RS20120 (AL468_11535) pilN 2276416..2276991 (+) 576 WP_046874634.1 PilN domain-containing protein Machinery gene
  AL468_RS20125 (AL468_11540) pilO 2276984..2277577 (+) 594 WP_012841351.1 type 4a pilus biogenesis protein PilO Machinery gene
  AL468_RS20130 (AL468_11545) pilP 2277567..2278082 (+) 516 WP_005395715.1 pilus assembly protein PilP Machinery gene
  AL468_RS20135 (AL468_11550) pilQ 2278108..2279856 (+) 1749 WP_006742777.1 type IV pilus secretin PilQ family protein Machinery gene
  AL468_RS20140 (AL468_11555) aroK 2280044..2280562 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37691.08 Da        Isoelectric Point: 6.9711

>NTDB_id=168574 AL468_RS20115 WP_005395718.1 2275416..2276432(+) (pilM) [Vibrio diabolicus strain FDAARGOS_99]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKLIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEDSEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEDGLRGTDNYQVFATRKDVVDSRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTSSSSFTMFTEQGELFHKEFACGTRGANSPVQDDLMSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAIRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=168574 AL468_RS20115 WP_005395718.1 2275416..2276432(+) (pilM) [Vibrio diabolicus strain FDAARGOS_99]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCTGATTAAAAAAGAACTGCCTTTTGGTGCTAAAAAGGTCGCAATTTCAGTGCCTGATAATTCG
GTGATCAGTAAAAAACTGCAAATAGAACAAAATCTTGAAGATAGCGAAGTTGAGTTTGCTGTGATTCAGGCATTTTCTCA
TCAATCGCCGTTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTCCGTCTTCGTGCCGAAGATGGGTTGCGTGGGACTGATA
ACTATCAAGTGTTTGCGACAAGAAAAGATGTGGTCGATAGTCGCGTGGATGCATTACAAAAATCCGGACTAAAACCCGTT
TTAGTGGACGTGCACTCACAAAGTCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAATAAGTACTG
CTTACTCGATATTGGCACCTCATCGAGCTCCTTTACCATGTTTACCGAACAAGGAGAGCTGTTTCACAAAGAGTTTGCTT
GCGGCACTCGAGGAGCAAACAGCCCTGTGCAAGATGATTTGATGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAT
CGCCAAATCGTGGAACGGGTGAAACGTCAGATGCAGCTCTACACCTCAATTAATGGCTCGCAAAATATCAAAGGTATCTG
GTTATCAGGGGAAGGAGCTTCGACACCAATGCTCGCGGAAGAGTTATCTCAGCAACTGTCACTAGAGTGTGAATTGCTAA
ACCCGCTAGGGCTATTTGAGATGAAGGTGTCCAAACGTAAACGTCGTGCCGCGGATTGGCAGCATTTCTCTACAGCCGCA
GGACTCGCGATTCGTGGCATTCATTGGCTAGGAGGTGCGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

82.544

100

0.825
  pilM Vibrio cholerae strain A1552

50.746

99.112

0.503

Multiple sequence alignment