Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AL541_RS14090 Genome accession   NZ_CP014040
Coordinates   2820873..2822618 (-) Length   581 a.a.
NCBI ID   WP_171053517.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain FDAARGOS_110     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2815873..2827618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL541_RS14070 (AL541_22440) - 2816561..2817400 (-) 840 WP_104979776.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  AL541_RS14075 (AL541_22445) - 2817470..2818981 (-) 1512 WP_025768079.1 SPOR domain-containing protein -
  AL541_RS14080 (AL541_22450) aroB 2819034..2820134 (-) 1101 WP_005381318.1 3-dehydroquinate synthase -
  AL541_RS14085 (AL541_22455) aroK 2820167..2820685 (-) 519 WP_005381319.1 shikimate kinase AroK -
  AL541_RS14090 (AL541_22460) pilQ 2820873..2822618 (-) 1746 WP_171053517.1 type IV pilus secretin PilQ Machinery gene
  AL541_RS14095 (AL541_22465) pilP 2822644..2823159 (-) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  AL541_RS14100 (AL541_22470) pilO 2823149..2823742 (-) 594 WP_064354357.1 type 4a pilus biogenesis protein PilO Machinery gene
  AL541_RS14105 (AL541_22475) pilN 2823735..2824310 (-) 576 WP_054577259.1 PilN domain-containing protein Machinery gene
  AL541_RS14110 (AL541_22480) pilM 2824294..2825310 (-) 1017 WP_017821733.1 type IV pilus biogenesis protein PilM Machinery gene

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63755.81 Da        Isoelectric Point: 4.4809

>NTDB_id=167840 AL541_RS14090 WP_171053517.1 2820873..2822618(-) (pilQ) [Vibrio alginolyticus strain FDAARGOS_110]
MKMRQGLTRTPRILTGLFSLWLFVFLAPLAAAEEVSSNALKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDEQLYLLDVKDFSTVVESVEVFRYTSTTRLVAHINEEYMHDYRLTGRFLEIKVAKIKPNEKAPDKSILEREGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQKLTEEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGIRWGFTSINGSNTVGGSIENNLATIGLYNGEGGDDGGDGDGVGIDDFLNVNLAATSPNAT
SIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVTFKKAVLSLKVTPQI
TPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFRR
SYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=167840 AL541_RS14090 WP_171053517.1 2820873..2822618(-) (pilQ) [Vibrio alginolyticus strain FDAARGOS_110]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATACTTACTGGGCTCTTCTCCTTATGGTTGTTTGTATTTTTAGC
CCCTTTAGCGGCAGCTGAAGAAGTTTCGTCCAATGCCCTAAAAAGTATCGACTTTCGAACTAATAAAGATAAAGACGCCG
TGATTGTCGTCGAATTGGCGTCACCGGCCGCGATTGTAGATATCAAGCGTGTACAGGAAGGGTTAAGTATCGATTTGCTT
AACACTTCAGTAAAAGACGAGCAATTGTATCTCCTAGACGTGAAAGACTTTTCGACTGTGGTTGAGAGCGTAGAAGTGTT
TCGCTATACCTCAACTACGCGCTTGGTTGCGCACATCAATGAAGAATACATGCATGATTACCGTTTAACGGGGCGTTTTT
TGGAAATCAAAGTAGCGAAGATCAAACCCAATGAGAAAGCCCCAGATAAAAGCATTTTAGAAAGAGAAGGTAAGCTTATT
TCGATTAACTTCCAAGATATTCCTGTGCGAAACGTCTTGCAGCTTATTGCTGATTACAACCAATTCAATTTGGTTGTCTC
CGACTCTGTAGAAGGCAACCTCACGCTTCGTCTTGATGGTGTCCCTTGGCAGCAAGTGCTGGATATTATTTTGCAAGTTA
AGGGGTTAGATAAGCGTGTTGATGGCAATGTGATTTTAGTTGCTCCGACTGATGAGTTGGACTTGCGTGAGAAGCAGCAG
TTAGAAAAACAAAAATTGACGGAAGAAATGGGAGAGCTTTCATCTGAAATCATCAAAGTTAATTTTGCTAAAGCATCAGA
TATTGCGGAGATGATCAATGGAGAAGGAAATATCAGCATGCTGTCAGAGCGCGGCAGTATGACCATTGATGAGCGAACTA
ACTCGTTATTGATTAGAGAGTTACCTGAAAACATCGAGGTGATTCGAGAAATTATTGAGTCTTTGGATATTCCAGTTAAA
CAGGTTCAAATTGAGGCGCGTATTGTGACCATCAATGAAGGTAACATGGACGAGTTAGGGATTCGTTGGGGTTTTACATC
TATTAATGGCAGTAATACAGTGGGCGGCTCTATTGAGAACAACCTCGCCACGATCGGTCTTTATAATGGAGAAGGGGGCG
ACGACGGTGGTGACGGTGATGGAGTGGGGATCGATGACTTCCTAAATGTAAACTTAGCTGCAACCAGCCCGAATGCGACC
AGCATTGCTTTTCAGGTCGCAAAGTTAGGTTCAGACACCTTGTTGGATCTTGAGCTTTCTGCTCTGCAACAAGAGTCTAA
AGCTGAGATTATCTCGAGCCCACGTTTGATCACTACCAATAAGAAACCAGCCTATATCGAACAAGGTACAGAGATTCCTT
ACCTAGAGTCATCATCAAGTGGTGCAACGACGGTGACGTTCAAAAAGGCGGTATTAAGTTTGAAGGTGACCCCGCAAATC
ACACCGGATAACCGCTTAGTGTTGGATTTAAGTGTCACGCAAGATAGACCTGGACAAGTCGTGAAGACAGGCACTGGTGA
AGCTGTTGCGATTGATACTCAACGTATCGGGACGCAAGTGTTAGTGAATAACGGTGAAACGGTCGTTCTTGGTGGTATTT
TTCAGCACAGCATTACTAACTCGGTGGACAAAGTGCCTTTGTTGGGCGATCTGCCGCTATTAGGTGCGCTTTTTCGTCGA
AGCTATGAAAATGTTGGCAAAAGTGAGCTGTTAATTTTCGTCACGCCTAAGGTGGTGATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(421-579)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

92.771

100

0.928
  pilQ Vibrio cholerae strain A1552

74.742

100

0.749
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.742

100

0.749
  pilQ Pseudomonas aeruginosa PAK

39.896

99.656

0.398
  pilQ Legionella pneumophila strain ERS1305867

40.608

96.213

0.391
  pilQ Legionella pneumophila strain Lp02

40.608

96.213

0.391
  comQ Acinetobacter baylyi ADP1

37.241

99.828

0.372
  pilQ Acinetobacter baumannii D1279779

36.074

100

0.37
  pilQ Acinetobacter baumannii strain A118

36.074

100

0.37

Multiple sequence alignment