Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ASZ85_RS12350 Genome accession   NZ_CP013317
Coordinates   2623124..2623585 (+) Length   153 a.a.
NCBI ID   WP_000649326.1    Uniprot ID   A0A0X1KZH2
Organism   Vibrio cholerae strain NCTC5395     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2618124..2628585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ASZ85_RS12325 (ASZ85_02408) dsbC 2618537..2619289 (-) 753 WP_000115055.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  ASZ85_RS12330 (ASZ85_02409) xerD 2619306..2620214 (-) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  ASZ85_RS12335 (ASZ85_02410) fldB 2620352..2620870 (+) 519 WP_000690112.1 flavodoxin FldB -
  ASZ85_RS12340 (ASZ85_02411) ampD 2621148..2621693 (-) 546 WP_000567318.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ASZ85_RS12345 (ASZ85_02412) nadC 2621989..2622879 (+) 891 WP_000665274.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ASZ85_RS12350 (ASZ85_02413) pilA 2623124..2623585 (+) 462 WP_000649326.1 pilin Machinery gene
  ASZ85_RS12355 (ASZ85_02414) pilB 2623585..2625273 (+) 1689 WP_000957200.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ASZ85_RS12360 (ASZ85_02415) pilC 2625316..2626542 (+) 1227 WP_000648511.1 type II secretion system F family protein Machinery gene
  ASZ85_RS12365 (ASZ85_02416) pilD 2626600..2627475 (+) 876 WP_000418747.1 prepilin peptidase Machinery gene
  ASZ85_RS12370 (ASZ85_02417) coaE 2627472..2628080 (+) 609 WP_000011557.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 15722.14 Da        Isoelectric Point: 8.9860

>NTDB_id=162015 ASZ85_RS12350 WP_000649326.1 2623124..2623585(+) (pilA) [Vibrio cholerae strain NCTC5395]
MKAYKNKQQKGFTLIELMIVVAVIGVLAAIAIPQYQNYVKKSAIGVGLANITALKTNIEDYIATEGSFPATTAGTAAGFT
RLGTVEDMGDGKIVIAPTASGALGGTIKYTFDAGVVSSSKIQLARDANGLWTCSTTVTSEIAPKGCTAGATIN

Nucleotide


Download         Length: 462 bp        

>NTDB_id=162015 ASZ85_RS12350 WP_000649326.1 2623124..2623585(+) (pilA) [Vibrio cholerae strain NCTC5395]
ATGAAAGCGTATAAAAACAAACAACAGAAAGGTTTTACCTTAATTGAATTGATGATTGTGGTGGCAGTGATTGGTGTGTT
GGCAGCGATTGCAATTCCTCAATATCAAAATTATGTAAAAAAATCAGCTATTGGGGTTGGCTTAGCAAACATCACAGCAC
TGAAAACTAATATTGAAGATTATATTGCGACAGAAGGCTCTTTTCCTGCAACAACTGCAGGTACGGCCGCAGGATTTACA
CGCTTAGGTACTGTTGAAGACATGGGTGACGGTAAGATTGTAATTGCCCCAACAGCATCAGGTGCATTAGGAGGTACAAT
AAAATACACATTCGATGCTGGTGTAGTATCTAGTTCAAAAATCCAATTAGCAAGAGATGCCAATGGATTATGGACTTGTA
GTACCACCGTTACTAGTGAAATAGCACCAAAAGGCTGTACTGCAGGTGCAACAATTAACTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0X1KZH2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

100

100

1
  pilA Vibrio cholerae strain A1552

100

100

1
  pilA Vibrio cholerae C6706

100

100

1
  pilA Pseudomonas aeruginosa PAK

42.675

100

0.438
  pilA Acinetobacter baumannii strain A118

42.667

98.039

0.418
  pilA Vibrio parahaemolyticus RIMD 2210633

41.892

96.732

0.405
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.86

93.464

0.373
  pilA2 Legionella pneumophila str. Paris

39.31

94.771

0.373
  pilA2 Legionella pneumophila strain ERS1305867

38.621

94.771

0.366

Multiple sequence alignment