Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ASZ85_RS12360 Genome accession   NZ_CP013317
Coordinates   2625316..2626542 (+) Length   408 a.a.
NCBI ID   WP_000648511.1    Uniprot ID   Q9X4G9
Organism   Vibrio cholerae strain NCTC5395     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2620316..2631542
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ASZ85_RS12335 (ASZ85_02410) fldB 2620352..2620870 (+) 519 WP_000690112.1 flavodoxin FldB -
  ASZ85_RS12340 (ASZ85_02411) ampD 2621148..2621693 (-) 546 WP_000567318.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ASZ85_RS12345 (ASZ85_02412) nadC 2621989..2622879 (+) 891 WP_000665274.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ASZ85_RS12350 (ASZ85_02413) pilA 2623124..2623585 (+) 462 WP_000649326.1 pilin Machinery gene
  ASZ85_RS12355 (ASZ85_02414) pilB 2623585..2625273 (+) 1689 WP_000957200.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ASZ85_RS12360 (ASZ85_02415) pilC 2625316..2626542 (+) 1227 WP_000648511.1 type II secretion system F family protein Machinery gene
  ASZ85_RS12365 (ASZ85_02416) pilD 2626600..2627475 (+) 876 WP_000418747.1 prepilin peptidase Machinery gene
  ASZ85_RS12370 (ASZ85_02417) coaE 2627472..2628080 (+) 609 WP_000011557.1 dephospho-CoA kinase -
  ASZ85_RS12375 (ASZ85_02418) zapD 2628112..2628852 (+) 741 WP_000207198.1 cell division protein ZapD -
  ASZ85_RS12380 (ASZ85_02419) yacG 2628994..2629191 (+) 198 WP_000162868.1 DNA gyrase inhibitor YacG -
  ASZ85_RS12385 (ASZ85_02420) parC 2629240..2631525 (-) 2286 WP_046121363.1 DNA topoisomerase IV subunit A -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45134.43 Da        Isoelectric Point: 10.3424

>NTDB_id=162017 ASZ85_RS12360 WP_000648511.1 2625316..2626542(+) (pilC) [Vibrio cholerae strain NCTC5395]
MKATQTLPLKNYRWKGINSNGKKVSGQMLAISEIEVRDKLKDQHIQIKKLKKGSVSLLARLTHRVKSKDITILTRQLATM
LTTGVPIVQALKLVGDNHRKAEMKSILAQITKSVEAGTPLSKAMRTASAHFDTLYVDLVETGEMSGNLPEVFERLATYRE
KSEQLRAKVIKALIYPSMVVLVALGVSYLMLTMVIPEFESMFKGFGAELPWFTQQVLKLSHWVQAYSLWAFIAIAAAIFG
LKALRKNSFQIRLKTSRLGLKFPIIGNVLAKASIAKFSRTLATSFAAGIPILASLKTTAKTSGNVHFETAINEVYRDTAA
GMPMYIAMRNTEAFPEMVLQMVMIGEESGQLDDMLNKVATIYEFEVDNTVDNLGKILEPLIIVFLGTVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=162017 ASZ85_RS12360 WP_000648511.1 2625316..2626542(+) (pilC) [Vibrio cholerae strain NCTC5395]
ATGAAAGCGACCCAAACCTTACCTCTGAAAAATTATCGCTGGAAAGGCATCAACAGCAACGGCAAAAAAGTTTCCGGCCA
GATGCTCGCCATCTCCGAAATCGAGGTGCGCGATAAGCTCAAAGATCAGCATATTCAGATCAAAAAACTCAAAAAAGGCA
GTGTATCTCTTTTGGCACGCCTAACCCATCGCGTGAAAAGTAAAGATATTACGATTTTGACTCGGCAGTTGGCGACCATG
CTCACCACGGGCGTACCCATTGTGCAAGCCCTCAAGTTGGTGGGCGATAATCACCGTAAAGCTGAGATGAAATCGATTCT
GGCGCAAATCACCAAAAGCGTGGAAGCGGGCACGCCACTTTCCAAGGCGATGCGCACCGCCAGCGCCCATTTTGATACCT
TGTATGTCGATTTAGTGGAAACCGGAGAGATGTCCGGTAACTTACCTGAGGTGTTTGAGCGTTTGGCCACCTACCGCGAG
AAAAGCGAGCAACTACGCGCCAAGGTGATTAAAGCGCTCATCTACCCCAGCATGGTTGTGTTGGTCGCGCTCGGGGTATC
TTACTTAATGCTCACCATGGTCATCCCAGAGTTTGAAAGCATGTTTAAAGGCTTTGGTGCTGAACTGCCTTGGTTTACGC
AGCAAGTGCTGAAACTCTCACACTGGGTGCAGGCTTACAGTTTATGGGCATTTATCGCCATCGCAGCAGCCATTTTTGGC
TTGAAAGCGCTGCGTAAAAACTCTTTCCAGATCCGTTTAAAAACCAGCCGCTTAGGGCTGAAATTTCCGATTATTGGTAA
TGTGCTCGCTAAGGCTTCCATCGCCAAATTCAGCCGTACCCTCGCCACCAGCTTTGCCGCGGGGATCCCAATTCTCGCCA
GTTTAAAAACCACGGCCAAAACCTCCGGCAATGTGCACTTTGAAACCGCGATTAATGAGGTGTACCGCGATACCGCTGCG
GGTATGCCGATGTACATTGCTATGCGCAATACCGAAGCTTTTCCCGAAATGGTGCTGCAAATGGTGATGATCGGTGAAGA
GTCTGGGCAATTAGACGACATGCTCAACAAGGTCGCGACCATCTATGAATTTGAAGTCGATAACACGGTCGATAACTTGG
GCAAGATTCTTGAACCACTGATCATCGTCTTTCTTGGGACGGTTGTGGGCGGCTTAGTGGTGGCGATGTACTTACCTATC
TTTAACTTAATGAGTGTATTGGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9X4G9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

100

100

1
  pilC Vibrio campbellii strain DS40M4

73.75

98.039

0.723
  pilC Acinetobacter baumannii D1279779

42.647

100

0.426
  pilC Acinetobacter baylyi ADP1

42.015

99.755

0.419
  pilC Legionella pneumophila strain ERS1305867

42.211

97.549

0.412
  pilC Pseudomonas stutzeri DSM 10701

42.172

97.059

0.409
  pilG Neisseria gonorrhoeae MS11

41.294

98.529

0.407
  pilG Neisseria meningitidis 44/76-A

40.796

98.529

0.402

Multiple sequence alignment