Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   NPD5_RS19200 Genome accession   NZ_CP013243
Coordinates   4014344..4014790 (+) Length   148 a.a.
NCBI ID   WP_045896601.1    Uniprot ID   A0A1J1CRC5
Organism   Clostridium sporogenes strain CDC_1632     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context


Location: 4009344..4019790
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPD5_RS19170 (NPD5_3801) - 4009708..4010298 (-) 591 WP_045896618.1 HD-GYP domain-containing protein -
  NPD5_RS19175 (NPD5_3802) - 4010430..4010885 (+) 456 WP_072586993.1 prepilin peptidase -
  NPD5_RS21850 (NPD5_3803) - 4010975..4011115 (+) 141 WP_003401881.1 hypothetical protein -
  NPD5_RS19180 (NPD5_3804) - 4011137..4011529 (+) 393 WP_072586994.1 LemA family protein -
  NPD5_RS19185 (NPD5_3805) - 4011555..4011740 (-) 186 WP_045896596.1 hypothetical protein -
  NPD5_RS19190 (NPD5_3806) - 4011821..4012849 (+) 1029 WP_072586995.1 NlpC/P60 family protein -
  NPD5_RS19195 (NPD5_3807) - 4013008..4014150 (+) 1143 WP_003362366.1 RNA-guided endonuclease InsQ/TnpB family protein -
  NPD5_RS19200 (NPD5_3808) ssbA 4014344..4014790 (+) 447 WP_045896601.1 single-stranded DNA-binding protein Machinery gene
  NPD5_RS19205 (NPD5_3809) - 4014926..4015159 (+) 234 WP_072586996.1 hypothetical protein -
  NPD5_RS19210 (NPD5_3810) - 4015178..4015822 (+) 645 WP_072586997.1 hypothetical protein -
  NPD5_RS19215 (NPD5_3811) - 4015876..4016346 (-) 471 WP_072586998.1 5'-nucleotidase -
  NPD5_RS19220 (NPD5_3812) - 4016706..4016933 (+) 228 WP_045897182.1 hypothetical protein -
  NPD5_RS19225 (NPD5_3814) - 4017405..4017686 (-) 282 WP_045897185.1 hypothetical protein -
  NPD5_RS19230 (NPD5_3815) - 4019026..4019235 (-) 210 WP_236905571.1 hypothetical protein -

Sequence


Protein


Download         Length: 148 a.a.        Molecular weight: 16445.26 Da        Isoelectric Point: 4.8013

>NTDB_id=161084 NPD5_RS19200 WP_045896601.1 4014344..4014790(+) (ssbA) [Clostridium sporogenes strain CDC_1632]
MNKVVLIGRLTKDPELKFTPGNGTAVANFTLAVDRRFSKDGQREADFIPIVVWGKQAESTANYMSKGKLMGVSGRIQTRS
YEAKDGTKRYITEVIAEEVKFLEWGDKATTDSSYNNRSESSYQSGQNFANGNSFDDDIIPVDDGDIPF

Nucleotide


Download         Length: 447 bp        

>NTDB_id=161084 NPD5_RS19200 WP_045896601.1 4014344..4014790(+) (ssbA) [Clostridium sporogenes strain CDC_1632]
ATGAATAAAGTTGTTTTAATAGGTAGACTAACCAAAGATCCTGAATTAAAATTTACTCCAGGAAATGGAACAGCGGTTGC
TAATTTCACTTTGGCTGTAGATAGAAGATTTTCAAAGGATGGTCAGAGAGAAGCAGATTTCATTCCTATAGTAGTATGGG
GAAAACAAGCAGAATCTACAGCTAACTATATGAGTAAAGGAAAACTTATGGGGGTTAGTGGTAGAATACAAACAAGAAGC
TATGAAGCTAAAGATGGTACAAAAAGATATATAACAGAAGTAATAGCTGAAGAAGTTAAATTTCTTGAGTGGGGAGATAA
AGCTACCACAGATTCATCATATAATAATAGATCAGAATCAAGTTACCAATCAGGACAAAATTTTGCAAATGGAAACAGTT
TTGACGATGATATAATTCCAGTAGATGATGGAGACATACCTTTTTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A1J1CRC5

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

46.512

100

0.541

  ssb Latilactobacillus sakei subsp. sakei 23K

41.216

100

0.412

  ssbB/cilA Streptococcus mitis NCTC 12261

39.189

100

0.392

  ssbB/cilA Streptococcus pneumoniae TIGR4

38.514

100

0.385

  ssbB/cilA Streptococcus pneumoniae Rx1

37.838

100

0.378

  ssbB/cilA Streptococcus pneumoniae D39

37.838

100

0.378

  ssbB/cilA Streptococcus pneumoniae R6

37.838

100

0.378

  ssbB/cilA Streptococcus mitis SK321

37.838

100

0.378

  ssbB Bacillus subtilis subsp. subtilis str. 168

54.369

69.595

0.378

  ssbB Streptococcus sobrinus strain NIDR 6715-7

52.427

69.595

0.365


Multiple sequence alignment