Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   SAST39_RS05260 Genome accession   NZ_CP012970
Coordinates   1068455..1068883 (+) Length   142 a.a.
NCBI ID   WP_000934385.1    Uniprot ID   A0A2K0CLZ8
Organism   Staphylococcus aureus strain ST20130939     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1055679..1103918 1068455..1068883 within 0


Gene organization within MGE regions


Location: 1055679..1103918
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SAST39_RS05140 (SAST39_03732) - 1055679..1056605 (-) 927 WP_000757565.1 glycerophosphodiester phosphodiesterase -
  SAST39_RS05145 (SAST39_02893) - 1056844..1057098 (+) 255 WP_001049150.1 YlbG family protein -
  SAST39_RS05150 (SAST39_01054) - 1057101..1057490 (-) 390 WP_000814565.1 hypothetical protein -
  SAST39_RS05155 (SAST39_01055) rsmD 1057560..1058102 (+) 543 WP_001263796.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  SAST39_RS05160 (SAST39_01056) coaD 1058104..1058586 (+) 483 WP_000401377.1 pantetheine-phosphate adenylyltransferase -
  SAST39_RS05165 (SAST39_01057) - 1058648..1059787 (-) 1140 WP_000843616.1 nucleotidyltransferase -
  SAST39_RS05170 (SAST39_01058) - 1059914..1060471 (+) 558 WP_000872155.1 YceD family protein -
  SAST39_RS05180 (SAST39_01059) rpmF 1060551..1060724 (+) 174 WP_000290472.1 50S ribosomal protein L32 -
  SAST39_RS05185 (SAST39_01060) - 1060841..1062226 (-) 1386 WP_000861313.1 recombinase family protein -
  SAST39_RS05190 (SAST39_01061) - 1062335..1062514 (+) 180 WP_000337827.1 hypothetical protein -
  SAST39_RS05195 (SAST39_01062) - 1062494..1063426 (-) 933 WP_000392183.1 hypothetical protein -
  SAST39_RS05200 (SAST39_01063) - 1063458..1063922 (-) 465 WP_000525007.1 hypothetical protein -
  SAST39_RS05205 (SAST39_03733) - 1063935..1064249 (-) 315 WP_000333630.1 helix-turn-helix domain-containing protein -
  SAST39_RS05210 (SAST39_02894) - 1064401..1064637 (+) 237 WP_001121116.1 helix-turn-helix domain-containing protein -
  SAST39_RS05215 (SAST39_01066) - 1064651..1064830 (+) 180 WP_000438352.1 hypothetical protein -
  SAST39_RS05220 (SAST39_01067) - 1064931..1065413 (-) 483 WP_000394410.1 hypothetical protein -
  SAST39_RS05225 (SAST39_01068) - 1065473..1066264 (+) 792 WP_001148563.1 phage antirepressor -
  SAST39_RS05230 (SAST39_01069) tscA 1066265..1066489 (+) 225 WP_000187184.1 type II toxin-antitoxin system antitoxin TscA -
  SAST39_RS14620 (SAST39_03174) - 1066529..1066705 (+) 177 WP_190362524.1 hypothetical protein -
  SAST39_RS05235 (SAST39_01071) - 1066680..1066910 (-) 231 WP_000395457.1 hypothetical protein -
  SAST39_RS14670 (SAST39_04290) - 1066960..1067097 (+) 138 WP_000230552.1 hypothetical protein -
  SAST39_RS05240 (SAST39_01073) - 1067090..1067251 (+) 162 WP_000066011.1 DUF1270 domain-containing protein -
  SAST39_RS05245 (SAST39_02895) - 1067348..1067608 (+) 261 WP_000291089.1 DUF1108 family protein -
  SAST39_RS05250 (SAST39_03175) - 1067618..1067839 (+) 222 WP_000815402.1 DUF2483 family protein -
  SAST39_RS05255 (SAST39_01076) - 1067832..1068455 (+) 624 WP_000139720.1 DUF1071 domain-containing protein -
  SAST39_RS05260 (SAST39_01077) ssbA 1068455..1068883 (+) 429 WP_000934385.1 single-stranded DNA-binding protein Machinery gene
  SAST39_RS05265 (SAST39_01078) - 1068897..1069565 (+) 669 WP_001124437.1 putative HNHc nuclease -
  SAST39_RS05270 (SAST39_03176) - 1069751..1070161 (-) 411 WP_000429731.1 hypothetical protein -
  SAST39_RS05275 (SAST39_01080) - 1070223..1070954 (+) 732 WP_000132051.1 helix-turn-helix domain-containing protein -
  SAST39_RS05280 (SAST39_01081) - 1070967..1071752 (+) 786 WP_000443353.1 ATP-binding protein -
  SAST39_RS05285 (SAST39_03177) - 1071749..1071907 (+) 159 WP_000628837.1 hypothetical protein -
  SAST39_RS05290 (SAST39_03178) - 1071920..1072141 (+) 222 WP_015984512.1 DUF3269 family protein -
  SAST39_RS05295 (SAST39_01083) - 1072151..1072555 (+) 405 WP_000049794.1 DUF1064 domain-containing protein -
  SAST39_RS05300 (SAST39_01084) - 1072560..1072745 (+) 186 WP_001187243.1 DUF3113 family protein -
  SAST39_RS05305 (SAST39_01085) - 1072746..1073051 (+) 306 WP_000101252.1 hypothetical protein -
  SAST39_RS05310 (SAST39_01086) - 1073179..1073535 (+) 357 WP_000029376.1 SA1788 family PVL leukocidin-associated protein -
  SAST39_RS05315 (SAST39_02896) - 1073539..1073781 (+) 243 WP_015984514.1 SAV1978 family virulence-associated passenger protein -
  SAST39_RS05320 (SAST39_01088) - 1073846..1074295 (+) 450 WP_000982708.1 YopX family protein -
  SAST39_RS05325 (SAST39_01089) - 1074292..1074657 (+) 366 WP_001624703.1 hypothetical protein -
  SAST39_RS05330 (SAST39_03734) - 1074650..1074904 (+) 255 WP_017804684.1 DUF1024 family protein -
  SAST39_RS05335 (SAST39_03735) - 1074891..1075061 (+) 171 WP_000714413.1 hypothetical protein -
  SAST39_RS05340 (SAST39_01092) - 1075054..1075590 (+) 537 WP_015984517.1 dUTPase -
  SAST39_RS05345 (SAST39_01093) - 1075627..1075821 (+) 195 WP_015984518.1 DUF1381 domain-containing protein -
  SAST39_RS05350 (SAST39_03736) - 1075821..1076345 (+) 525 WP_015984519.1 hypothetical protein -
  SAST39_RS05355 (SAST39_01095) rinB 1076392..1076565 (+) 174 WP_000595258.1 transcriptional activator RinB -
  SAST39_RS05360 (SAST39_01096) - 1076566..1076712 (+) 147 WP_000990005.1 hypothetical protein -
  SAST39_RS05365 (SAST39_01097) - 1076736..1077158 (+) 423 WP_000162701.1 RinA family phage transcriptional activator -
  SAST39_RS05370 (SAST39_04222) - 1077489..1077983 (+) 495 WP_000594088.1 terminase small subunit -
  SAST39_RS05375 (SAST39_01099) - 1077976..1079199 (+) 1224 WP_001037578.1 PBSX family phage terminase large subunit -
  SAST39_RS05380 (SAST39_01100) - 1079196..1080620 (+) 1425 WP_000177422.1 phage portal protein -
  SAST39_RS05385 (SAST39_04291) - 1080589..1081542 (+) 954 WP_015984520.1 phage head morphogenesis protein -
  SAST39_RS05390 (SAST39_01102) - 1081544..1081750 (+) 207 WP_000346032.1 hypothetical protein -
  SAST39_RS05395 (SAST39_01103) - 1081853..1082437 (+) 585 WP_001019221.1 DUF4355 domain-containing protein -
  SAST39_RS05400 (SAST39_01104) - 1082454..1083368 (+) 915 WP_000235168.1 phage major capsid protein -
  SAST39_RS14610 (SAST39_03183) - 1083380..1083523 (+) 144 WP_000002931.1 hypothetical protein -
  SAST39_RS05405 (SAST39_03738) - 1083529..1083879 (+) 351 WP_000177351.1 phage head-tail adapter protein -
  SAST39_RS05410 (SAST39_01106) - 1083891..1084226 (+) 336 WP_000483040.1 phage head closure protein -
  SAST39_RS05415 (SAST39_01107) - 1084213..1084626 (+) 414 WP_001151330.1 HK97-gp10 family putative phage morphogenesis protein -
  SAST39_RS05420 (SAST39_03185) - 1084639..1085064 (+) 426 WP_000270192.1 DUF3168 domain-containing protein -
  SAST39_RS05425 (SAST39_01109) - 1085065..1085622 (+) 558 WP_000057582.1 tail protein -
  SAST39_RS05430 (SAST39_01110) - 1085688..1086194 (+) 507 WP_000134337.1 tail assembly chaperone -
  SAST39_RS05435 (SAST39_01111) - 1086239..1086523 (+) 285 WP_000880587.1 hypothetical protein -
  SAST39_RS05440 (SAST39_03186) - 1086803..1089496 (+) 2694 Protein_1049 terminase -
  SAST39_RS05445 (SAST39_03187) - 1089511..1090452 (+) 942 WP_062911908.1 phage tail domain-containing protein -
  SAST39_RS05450 (SAST39_03188) - 1090463..1092346 (+) 1884 WP_062911909.1 SGNH/GDSL hydrolase family protein -
  SAST39_RS05455 (SAST39_03189) - 1092359..1094257 (+) 1899 WP_062911910.1 hypothetical protein -
  SAST39_RS05460 (SAST39_01116) - 1094257..1096080 (+) 1824 WP_023487056.1 phage baseplate upper protein -
  SAST39_RS05465 (SAST39_01117) - 1096080..1096457 (+) 378 WP_000705893.1 DUF2977 domain-containing protein -
  SAST39_RS05470 (SAST39_01118) - 1096461..1096634 (+) 174 WP_001790193.1 XkdX family protein -
  SAST39_RS05475 (SAST39_01119) - 1096675..1096974 (+) 300 WP_000466769.1 DUF2951 domain-containing protein -
  SAST39_RS05480 (SAST39_03739) - 1097111..1098985 (+) 1875 WP_000524043.1 glucosaminidase domain-containing protein -
  SAST39_RS05485 (SAST39_01122) - 1098998..1100170 (+) 1173 WP_062911911.1 BppU family phage baseplate upper protein -
  SAST39_RS05490 (SAST39_01123) - 1100176..1100571 (+) 396 WP_000398878.1 hypothetical protein -
  SAST39_RS05495 (SAST39_01124) - 1100627..1101064 (+) 438 WP_000354132.1 phage holin -
  SAST39_RS05500 (SAST39_01125) - 1101045..1102490 (+) 1446 WP_001148137.1 SH3 domain-containing protein -
  SAST39_RS05505 (SAST39_01126) - 1102879..1103466 (+) 588 WP_000448197.1 hypothetical protein -
  SAST39_RS05510 (SAST39_03190) - 1103463..1103918 (+) 456 WP_000971220.1 hypothetical protein -

Sequence


Protein


Download         Length: 142 a.a.        Molecular weight: 15872.58 Da        Isoelectric Point: 8.4825

>NTDB_id=158687 SAST39_RS05260 WP_000934385.1 1068455..1068883(+) (ssbA) [Staphylococcus aureus strain ST20130939]
MLNRAVLVGRLTKDPELRSAPNGVNVGTFTLAVNRTFTNAQGEREADFINVVVFKKQAENVKNYLSKGSLAGVDGRLQTR
SYENKVGQRVFVTEVVADSVQFLEPKNNNQQPNNNYHQQRQTQTGNNPFDNTTAITDDDLPF

Nucleotide


Download         Length: 429 bp        

>NTDB_id=158687 SAST39_RS05260 WP_000934385.1 1068455..1068883(+) (ssbA) [Staphylococcus aureus strain ST20130939]
ATGTTAAACAGAGCAGTATTAGTAGGACGCTTAACAAAAGACCCAGAATTAAGAAGCGCGCCAAATGGCGTAAATGTAGG
TACATTCACATTGGCAGTAAACAGAACATTCACGAATGCTCAAGGCGAGCGTGAAGCAGATTTTATAAACGTAGTAGTGT
TCAAGAAACAAGCTGAAAATGTTAAAAACTACCTTTCTAAAGGGTCGCTGGCAGGTGTAGACGGGCGACTACAAACACGT
AGCTACGAAAATAAAGTCGGGCAACGTGTATTTGTGACAGAAGTAGTAGCGGACAGTGTTCAATTCTTAGAACCGAAGAA
TAACAACCAACAACCAAACAACAATTATCATCAACAAAGACAAACTCAAACTGGTAATAATCCTTTTGATAATACCACTG
CGATTACTGATGATGACTTACCGTTCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A2K0CLZ8

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

58.14

100

0.704

  ssb Latilactobacillus sakei subsp. sakei 23K

50.588

100

0.606

  ssbB Bacillus subtilis subsp. subtilis str. 168

58.491

74.648

0.437

  ssbB Streptococcus sobrinus strain NIDR 6715-7

41.958

100

0.423

  ssbA Streptococcus mutans UA159

40.845

100

0.408

  ssbB/cilA Streptococcus mitis NCTC 12261

40.141

100

0.401

  ssbB/cilA Streptococcus pneumoniae TIGR4

40.141

100

0.401

  ssbB/cilA Streptococcus pneumoniae Rx1

39.437

100

0.394

  ssbB/cilA Streptococcus pneumoniae D39

39.437

100

0.394

  ssbB/cilA Streptococcus pneumoniae R6

39.437

100

0.394

  ssbB/cilA Streptococcus mitis SK321

39.437

100

0.394


Multiple sequence alignment