Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AO356_RS16030 Genome accession   NZ_CP012831
Coordinates   3618104..3619321 (-) Length   405 a.a.
NCBI ID   WP_060740579.1    Uniprot ID   A0A2K4FK57
Organism   Pseudomonas fluorescens strain FW300-N2C3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3613104..3624321
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO356_RS15990 (AO356_15970) - 3613935..3614108 (-) 174 WP_039593877.1 DUF3094 family protein -
  AO356_RS15995 (AO356_15975) - 3614161..3614688 (-) 528 WP_060740575.1 MOSC domain-containing protein -
  AO356_RS16000 (AO356_15980) - 3614685..3615311 (-) 627 WP_025215531.1 DUF1780 domain-containing protein -
  AO356_RS16005 (AO356_15985) - 3615445..3616131 (+) 687 WP_060740576.1 energy-coupling factor ABC transporter permease -
  AO356_RS16010 (AO356_15990) - 3616195..3616410 (+) 216 WP_053125334.1 hypothetical protein -
  AO356_RS16015 (AO356_15995) yacG 3616407..3616613 (-) 207 WP_057450865.1 DNA gyrase inhibitor YacG -
  AO356_RS16020 (AO356_16000) coaE 3616610..3617233 (-) 624 WP_060740577.1 dephospho-CoA kinase -
  AO356_RS16025 (AO356_16005) pilD 3617233..3618102 (-) 870 WP_060740578.1 prepilin peptidase Machinery gene
  AO356_RS16030 (AO356_16010) pilC 3618104..3619321 (-) 1218 WP_060740579.1 type II secretion system F family protein Machinery gene
  AO356_RS16035 (AO356_16015) pilB 3619324..3621033 (-) 1710 WP_060740580.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AO356_RS16040 (AO356_16020) - 3621257..3621667 (+) 411 WP_060743129.1 pilin -
  AO356_RS16045 (AO356_16025) - 3621936..3622289 (+) 354 WP_053125323.1 BON domain-containing protein -
  AO356_RS16050 (AO356_16030) - 3622620..3622916 (-) 297 WP_060740581.1 DUF2845 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44322.98 Da        Isoelectric Point: 9.9619

>NTDB_id=157823 AO356_RS16030 WP_060740579.1 3618104..3619321(-) (pilC) [Pseudomonas fluorescens strain FW300-N2C3]
MAVKAVKTDVYTWEGKDRKGAKMTGELTGQSPALVKAQLRKQGINPEKVRKKSTSIFSKGKRIKPLDIALFTRQMATMLK
AGVPLLQAFDIIGEGFDNANMRKLVDQVKQEVAAGNSFAASLRKCPQYFDDLYCNLVDAGEQAGALDTLLDRVATYKEKS
EALKAKIKKAMTYPTAVILVAAVVTGILLVKVVPQFESVFSGFGAQLPAFTVMVIGLSEFLQDWWWVVLGGLVGTFFGVK
YALRRSERFRDWRDKWLLKLPLIGALMYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKRAVQRIRQDVSTGM
QLNFSMRASGIFPNLAIQMTAIGEESGALDDMLDKVASFYEAEVDNLVDNLTSLMEPFIMVILGVVVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=157823 AO356_RS16030 WP_060740579.1 3618104..3619321(-) (pilC) [Pseudomonas fluorescens strain FW300-N2C3]
ATGGCGGTCAAGGCAGTAAAAACCGACGTCTATACGTGGGAAGGCAAAGACCGCAAAGGCGCGAAAATGACCGGCGAGCT
GACGGGACAGAGCCCGGCCCTGGTCAAGGCACAACTGCGCAAACAAGGCATCAACCCGGAGAAGGTGCGCAAGAAATCCA
CCTCGATATTCAGCAAGGGCAAGCGCATCAAGCCGCTGGATATCGCCCTCTTCACCCGCCAGATGGCAACGATGCTCAAG
GCTGGCGTGCCGCTGTTGCAGGCGTTCGACATCATCGGCGAAGGCTTCGACAACGCCAACATGCGCAAGCTGGTGGACCA
GGTGAAACAGGAAGTCGCCGCCGGCAACAGCTTCGCCGCCTCGCTGCGCAAATGCCCGCAGTACTTCGACGATTTGTACT
GCAACCTGGTGGATGCCGGTGAACAGGCCGGTGCCCTGGATACGCTGCTGGATCGGGTCGCGACCTACAAGGAAAAGAGC
GAAGCGCTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCGACGGCGGTGATACTCGTCGCTGCGGTGGTCACCGGCAT
CCTGCTGGTCAAGGTGGTGCCGCAGTTCGAATCGGTCTTTTCAGGGTTCGGTGCACAGCTACCGGCCTTCACGGTAATGG
TCATCGGCCTGTCGGAATTCTTGCAGGATTGGTGGTGGGTGGTGCTTGGCGGGCTTGTAGGGACATTTTTTGGCGTGAAA
TACGCCCTCAGGCGTTCCGAGCGCTTTCGTGACTGGCGCGATAAATGGCTACTCAAACTACCGCTGATAGGCGCCTTGAT
GTACAAGTCCGCCGTGGCCCGCTACGCCCGCACGCTCTCGACGACATTCGCCGCCGGCGTGCCGCTGGTCGAAGCCCTCG
ACTCGGTATCGGGCGCCACCGGCAACGTGGTGTTCAAGCGCGCTGTGCAGCGCATCCGCCAGGACGTCTCCACTGGCATG
CAGTTGAATTTTTCCATGCGCGCGTCAGGGATCTTTCCGAACCTGGCCATCCAGATGACCGCCATCGGCGAGGAATCCGG
CGCGCTGGACGACATGCTCGACAAAGTGGCGAGTTTTTATGAGGCCGAAGTGGACAATCTGGTGGACAACCTCACCAGCC
TGATGGAACCCTTCATCATGGTGATCCTGGGGGTCGTCGTGGGTGGCCTGGTAGTCGCCATGTACCTGCCCATCTTTCAA
CTCGGCTCTGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K4FK57

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

75.062

100

0.751
  pilC Acinetobacter baylyi ADP1

59.548

98.272

0.585
  pilC Acinetobacter baumannii D1279779

57.598

100

0.58
  pilC Legionella pneumophila strain ERS1305867

54.798

97.778

0.536
  pilG Neisseria gonorrhoeae MS11

43.035

99.259

0.427
  pilC Vibrio campbellii strain DS40M4

43.434

97.778

0.425
  pilC Vibrio cholerae strain A1552

42.929

97.778

0.42
  pilG Neisseria meningitidis 44/76-A

42.04

99.259

0.417
  pilC Thermus thermophilus HB27

37.157

99.012

0.368

Multiple sequence alignment