Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AO356_RS16035 Genome accession   NZ_CP012831
Coordinates   3619324..3621033 (-) Length   569 a.a.
NCBI ID   WP_060740580.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain FW300-N2C3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3614324..3626033
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO356_RS16000 (AO356_15980) - 3614685..3615311 (-) 627 WP_025215531.1 DUF1780 domain-containing protein -
  AO356_RS16005 (AO356_15985) - 3615445..3616131 (+) 687 WP_060740576.1 energy-coupling factor ABC transporter permease -
  AO356_RS16010 (AO356_15990) - 3616195..3616410 (+) 216 WP_053125334.1 hypothetical protein -
  AO356_RS16015 (AO356_15995) yacG 3616407..3616613 (-) 207 WP_057450865.1 DNA gyrase inhibitor YacG -
  AO356_RS16020 (AO356_16000) coaE 3616610..3617233 (-) 624 WP_060740577.1 dephospho-CoA kinase -
  AO356_RS16025 (AO356_16005) pilD 3617233..3618102 (-) 870 WP_060740578.1 prepilin peptidase Machinery gene
  AO356_RS16030 (AO356_16010) pilC 3618104..3619321 (-) 1218 WP_060740579.1 type II secretion system F family protein Machinery gene
  AO356_RS16035 (AO356_16015) pilB 3619324..3621033 (-) 1710 WP_060740580.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AO356_RS16040 (AO356_16020) - 3621257..3621667 (+) 411 WP_060743129.1 pilin -
  AO356_RS16045 (AO356_16025) - 3621936..3622289 (+) 354 WP_053125323.1 BON domain-containing protein -
  AO356_RS16050 (AO356_16030) - 3622620..3622916 (-) 297 WP_060740581.1 DUF2845 domain-containing protein -
  AO356_RS16055 (AO356_16035) - 3622918..3625851 (-) 2934 WP_203225753.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63034.15 Da        Isoelectric Point: 5.4265

>NTDB_id=157824 AO356_RS16035 WP_060740580.1 3619324..3621033(-) (pilB) [Pseudomonas fluorescens strain FW300-N2C3]
MNDIALSGLTKQLVLAELLTEQSAQQAYQQAQRSRIPLVSYLVQNKLVQSRQVAEIASEHFGVALLDLNSLDKDTQPTGL
VSEKLVRQHHVLPLWRRGNKLFVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESNSTGLEGMGDVDLDG
LDGLDIESIDDTKQDSIGNQDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRVRTDGMLREVARPPIQLATRIA
ARLKVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQ
PQGLILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEI
RDLETAEIAIKAAQTGHLVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCAHCKKAIEIPEETLLKEG
FPRERIGTFTIYEPVGCQQCNHGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKVMQGVTSL
EEINRVTKD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=157824 AO356_RS16035 WP_060740580.1 3619324..3621033(-) (pilB) [Pseudomonas fluorescens strain FW300-N2C3]
ATGAATGACATCGCCCTCAGCGGCCTGACCAAGCAATTGGTGCTGGCTGAACTGCTCACCGAGCAAAGTGCGCAACAGGC
GTATCAACAGGCCCAACGCAGTCGCATCCCCCTGGTCAGCTACCTGGTGCAGAACAAACTGGTCCAGAGCCGCCAGGTCG
CGGAGATCGCCTCGGAGCATTTCGGCGTCGCCCTGCTGGACCTCAACAGCCTGGACAAGGACACCCAACCCACGGGCCTG
GTCAGCGAAAAACTGGTGCGCCAACATCACGTCCTGCCGCTGTGGCGACGCGGCAACAAGTTGTTCGTGGGCATCTCCGA
CCCCACCAATCACCAAGCCATCAATGACATTCAATTCAGTACCGGCCTGACCACCGAAGCCATCCTGGTAGAAGACGACA
AGCTCAGCGACGCCATCGAAAAGTTTTTCGAATCCAATAGCACGGGCCTGGAAGGCATGGGCGATGTCGACCTGGACGGT
CTGGACGGTCTGGACATCGAATCGATCGACGACACCAAGCAGGATTCCATCGGCAACCAGGACGCCGACGACGCGCCCGT
GGTGCGTTTCGTCAACAAGATGCTGCTGGACGCGATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCTTACGAAAAAA
TCTACCGGGTGCGGGTGCGCACCGACGGCATGCTGCGGGAAGTGGCCCGGCCGCCGATCCAACTGGCGACCCGTATCGCC
GCCCGCCTGAAAGTCATGGCCAGCCTCGATATTTCCGAACGGCGCAAACCCCAGGACGGCCGGCTGAAAATGCGCCTGTC
GAAAACCAAGTCCATCGACTTCCGGGTCAACACCCTGCCGACGCTCTGGGGCGAAAAAGTGGTGATCCGGATCCTCGACC
CCTCCAGTGCGCAAATGGGCATCGATGCCCTGGGCTACGAGCCGGATCAGAAAGACCTGTACATGGCCGCCCTCAAACAG
CCACAAGGGCTGATCCTGGTCACCGGCCCGACCGGCTCGGGCAAGACCGTGTCGCTGTACACCGGGCTGAATATCCTCAA
TACCGTGGACATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTGAACC
CGCGCCAGGGGCTGGACTTCGCCCAGGCCCTGCGCTCATTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATC
CGCGACCTGGAAACCGCCGAAATCGCCATCAAGGCCGCCCAGACCGGGCACCTGGTGCTCTCCACACTGCACACCAACAG
CGCCGCCGAAACCCTGATCCGCTTGCAGAACATGGGCATTCCAGGGTTCAACATCGCCACCGCCGTGCACCTGATCATCG
CCCAGCGGTTGGCGCGCAAGCTCTGCGCCCACTGCAAGAAAGCCATCGAGATTCCCGAGGAAACCCTGCTCAAGGAAGGT
TTCCCCCGGGAACGCATCGGCACATTCACGATCTATGAGCCGGTCGGTTGCCAACAGTGCAACCACGGTTACAAAGGGCG
CGTGGGGGTTTACGAAGTGGTCAAGAACACGCCCGAGCTGCAACGGTTGATCATGGCCGAGGGCAACTCGCTGGAAATCG
ATCTGCAAATGCGCAAGGACGGCTTCAATGACCTGCGCACATCGGGGCTGCTCAAAGTGATGCAGGGCGTCACCAGCCTC
GAGGAAATCAACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.451

99.824

0.583
  pilB Acinetobacter baumannii D1279779

56.966

99.649

0.568
  pilB Legionella pneumophila strain ERS1305867

55.282

99.824

0.552
  pilB Vibrio cholerae strain A1552

50.088

99.649

0.499
  pilB Vibrio campbellii strain DS40M4

48.862

100

0.49
  pilF Neisseria gonorrhoeae MS11

48.41

99.473

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

49.273

96.661

0.476

Multiple sequence alignment