Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AO353_RS14325 Genome accession   NZ_CP012830
Coordinates   3174301..3175518 (-) Length   405 a.a.
NCBI ID   WP_054595546.1    Uniprot ID   A0A2N8BX06
Organism   Pseudomonas fluorescens strain FW300-N2E3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3169301..3180518
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO353_RS32150 (AO353_14285) - 3169740..3170189 (+) 450 WP_371917641.1 methyl-accepting chemotaxis protein -
  AO353_RS14290 (AO353_14290) - 3170210..3170449 (-) 240 WP_054595539.1 DUF2790 domain-containing protein -
  AO353_RS14295 (AO353_14295) - 3170571..3171503 (+) 933 WP_054595540.1 LysR family transcriptional regulator -
  AO353_RS14300 (AO353_14300) - 3171634..3172320 (+) 687 WP_054595541.1 energy-coupling factor ABC transporter permease -
  AO353_RS14305 (AO353_14305) - 3172384..3172599 (+) 216 WP_054595542.1 hypothetical protein -
  AO353_RS14310 (AO353_14310) yacG 3172609..3172809 (-) 201 WP_054595543.1 DNA gyrase inhibitor YacG -
  AO353_RS14315 (AO353_14315) coaE 3172806..3173429 (-) 624 WP_054595544.1 dephospho-CoA kinase -
  AO353_RS14320 (AO353_14320) pilD 3173426..3174298 (-) 873 WP_054595545.1 prepilin peptidase Machinery gene
  AO353_RS14325 (AO353_14325) pilC 3174301..3175518 (-) 1218 WP_054595546.1 type II secretion system F family protein Machinery gene
  AO353_RS14330 (AO353_14330) pilB 3175522..3177222 (-) 1701 WP_054595547.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AO353_RS14335 (AO353_14335) - 3177449..3177859 (+) 411 WP_054595548.1 pilin -
  AO353_RS14340 (AO353_14340) - 3178137..3178493 (+) 357 WP_054595549.1 BON domain-containing protein -
  AO353_RS14345 (AO353_14345) - 3178605..3178904 (-) 300 WP_054595550.1 DUF2845 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44269.31 Da        Isoelectric Point: 10.0044

>NTDB_id=157788 AO353_RS14325 WP_054595546.1 3174301..3175518(-) (pilC) [Pseudomonas fluorescens strain FW300-N2E3]
MAVKAIKVSVYAWEGTDRKGSKVTGELSGHNPALIKAQLRKQGINPGKVRKKTASLFNLGNRIKPLDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPNMRKLVDEVKQEVAAGNSFATALRKKPQYFDELYCNLVDAGEQAGALDTLLERIATYKEKS
ESLKAKIKKAMTYPLAVVFVAIIVTGILLVKVVPQFQSVFSGFGAELPAFTLMVIGLSEFMQAWWWLLLGGLIIGALSFR
HALKRSQRFCDWLDAKLLKMPILGALLYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNIVFKRAVLRIKQDVSTGM
QLNFSMRTSRIFPNMAIQMAAIGEESGALDDMLDKVAIYYEAEVDNMVDSLTSLLEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=157788 AO353_RS14325 WP_054595546.1 3174301..3175518(-) (pilC) [Pseudomonas fluorescens strain FW300-N2E3]
ATGGCGGTCAAAGCAATCAAGGTCAGCGTCTACGCCTGGGAAGGCACCGACAGGAAAGGCAGCAAGGTAACCGGCGAGCT
GAGCGGTCATAACCCGGCCTTGATCAAGGCGCAGCTACGCAAACAAGGGATCAACCCGGGCAAAGTACGGAAAAAAACCG
CTTCGCTGTTCAACCTCGGCAACCGTATCAAGCCGCTGGACATTGCTCTCTTCACCCGGCAAATGGCGACCATGATGAAA
GCCGGTGTGCCGCTGCTGCAGTCGTTCGACATCATCGGCGAGGGCTTCGACAACCCGAACATGCGCAAGCTGGTGGACGA
GGTGAAACAGGAAGTCGCCGCCGGCAACAGCTTCGCCACCGCACTGCGGAAAAAGCCGCAGTACTTCGATGAGCTCTACT
GCAACCTGGTCGACGCGGGCGAGCAGGCCGGCGCGCTCGACACGCTGCTGGAGCGGATTGCGACCTACAAGGAAAAAAGC
GAAAGCCTCAAGGCCAAGATCAAAAAAGCCATGACCTATCCGCTGGCCGTGGTCTTTGTCGCAATCATCGTCACCGGCAT
TCTGCTGGTCAAAGTGGTCCCGCAATTTCAGTCGGTGTTTTCCGGATTTGGTGCAGAACTCCCGGCCTTTACCCTGATGG
TGATCGGTCTGTCCGAGTTCATGCAGGCGTGGTGGTGGCTGTTACTCGGCGGCCTGATCATCGGGGCCCTGAGCTTTCGC
CATGCGCTCAAGCGCTCGCAACGCTTTTGCGATTGGCTGGATGCCAAACTGCTGAAAATGCCGATACTCGGCGCACTGCT
CTACAAATCGGCGGTGGCGCGCTTTGCCCGTACCCTGTCGACCACCTTCGCCGCAGGCGTCCCCCTGGTCGAAGCTCTGG
ATTCGGTAGCAGGTGCTACTGGCAATATCGTGTTCAAACGGGCGGTTCTGCGCATCAAACAGGATGTCTCCACCGGCATG
CAATTGAACTTCTCGATGCGCACCAGCAGGATTTTCCCCAACATGGCGATTCAAATGGCCGCCATTGGCGAGGAGTCTGG
CGCACTCGACGATATGCTCGACAAAGTTGCGATCTACTATGAAGCTGAAGTCGACAATATGGTCGACAGCCTGACCAGCC
TGTTGGAGCCGTTCATCATGGTGGTGCTCGGCGTCATTGTCGGCGGGCTGGTGGTTGCCATGTACCTGCCCATTTTCCAA
CTCGGTTCAGCGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N8BX06

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.568

100

0.746
  pilC Acinetobacter baylyi ADP1

60.244

100

0.61
  pilC Acinetobacter baumannii D1279779

58.088

100

0.585
  pilC Legionella pneumophila strain ERS1305867

54.04

97.778

0.528
  pilG Neisseria gonorrhoeae MS11

43.176

99.506

0.43
  pilC Vibrio cholerae strain A1552

42.402

100

0.427
  pilG Neisseria meningitidis 44/76-A

42.928

99.506

0.427
  pilC Vibrio campbellii strain DS40M4

42.68

99.506

0.425
  pilC Thermus thermophilus HB27

38.155

99.012

0.378

Multiple sequence alignment