Detailed information NTG0157787

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AO353_RS14320
• Genome accession: NZ_CP012830
• Coordinates: 3173426..3174298 (-)
• Length: 290 a.a.
• NCBI ID: WP_054595545.1
• Uniprot ID: -
• Organism: Pseudomonas fluorescens strain FW300-N2E3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3168426..3179298

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AO353_RS32150 (AO353_14285)	-	3169740..3170189 (+)	450	WP_371917641.1	methyl-accepting chemotaxis protein	-
AO353_RS14290 (AO353_14290)	-	3170210..3170449 (-)	240	WP_054595539.1	DUF2790 domain-containing protein	-
AO353_RS14295 (AO353_14295)	-	3170571..3171503 (+)	933	WP_054595540.1	LysR family transcriptional regulator	-
AO353_RS14300 (AO353_14300)	-	3171634..3172320 (+)	687	WP_054595541.1	energy-coupling factor ABC transporter permease	-
AO353_RS14305 (AO353_14305)	-	3172384..3172599 (+)	216	WP_054595542.1	hypothetical protein	-
AO353_RS14310 (AO353_14310)	yacG	3172609..3172809 (-)	201	WP_054595543.1	DNA gyrase inhibitor YacG	-
AO353_RS14315 (AO353_14315)	coaE	3172806..3173429 (-)	624	WP_054595544.1	dephospho-CoA kinase	-
AO353_RS14320 (AO353_14320)	pilD	3173426..3174298 (-)	873	WP_054595545.1	prepilin peptidase	Machinery gene
AO353_RS14325 (AO353_14325)	pilC	3174301..3175518 (-)	1218	WP_054595546.1	type II secretion system F family protein	Machinery gene
AO353_RS14330 (AO353_14330)	pilB	3175522..3177222 (-)	1701	WP_054595547.1	type IV-A pilus assembly ATPase PilB	Machinery gene
AO353_RS14335 (AO353_14335)	-	3177449..3177859 (+)	411	WP_054595548.1	pilin	-
AO353_RS14340 (AO353_14340)	-	3178137..3178493 (+)	357	WP_054595549.1	BON domain-containing protein	-
AO353_RS14345 (AO353_14345)	-	3178605..3178904 (-)	300	WP_054595550.1	DUF2845 domain-containing protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31864.98 Da
• Isoelectric Point: 7.6628

>NTDB_id=157787 AO353_RS14320 WP_054595545.1 3173426..3174298(-) (pilD) [Pseudomonas fluorescens strain FW300-N2E3]
MPLSEFMAGYPLAFVVTALVLGLVIGSFLNVVVWRLPKMLEGDWRQQALDVLGLPPEAPGPTYNLMLPHSHCPQCAHRIR
AWENIPLLSYLALRGRCSNCKAPISARYPLTELACGVLSAFVAWHFGFGWTAAWVLVLSWGLLAMCLIDADHQLLPDVLV
LPLLWLGLIVNSFELFVPLQAAMWGAVAGYLLLWSVYWLFKLITGKEGIGHGDFKLLALLGAWGGWQILPLTVLLSSLVG
AVGGVILLRLKDTNTSTPIPFGPYLAIAGWIALLWGGQITGFYWQFVGFK

Nucleotide

• Length: 873 bp

>NTDB_id=157787 AO353_RS14320 WP_054595545.1 3173426..3174298(-) (pilD) [Pseudomonas fluorescens strain FW300-N2E3]
ATGCCCTTGAGTGAATTCATGGCCGGTTACCCACTGGCATTCGTTGTCACTGCGCTAGTGCTTGGGCTGGTGATCGGTAG
TTTTCTCAACGTGGTAGTCTGGCGCCTGCCGAAAATGCTTGAGGGCGACTGGCGGCAACAAGCCCTGGATGTACTCGGTT
TACCCCCTGAAGCACCGGGCCCGACCTACAACCTGATGTTGCCGCATTCACACTGCCCGCAGTGCGCCCATCGAATCCGC
GCCTGGGAAAACATTCCGCTGCTCAGTTACCTGGCACTGCGCGGCCGGTGCTCAAATTGCAAAGCGCCGATCAGCGCCCG
CTATCCCTTGACCGAACTGGCCTGCGGCGTGCTGTCGGCGTTTGTCGCCTGGCACTTCGGCTTCGGTTGGACAGCTGCGT
GGGTGCTGGTCCTGAGTTGGGGGTTATTGGCGATGTGCCTGATCGACGCCGACCACCAATTGCTGCCGGATGTGCTGGTG
TTGCCGTTGTTGTGGCTGGGTCTGATCGTCAACAGCTTCGAGCTTTTCGTGCCTTTGCAGGCGGCGATGTGGGGGGCGGT
GGCCGGCTATCTGCTGCTGTGGTCGGTGTATTGGCTGTTCAAACTGATAACCGGCAAGGAAGGTATCGGTCACGGCGACT
TCAAGCTCCTGGCGCTGCTCGGTGCCTGGGGCGGTTGGCAGATTCTGCCGCTGACGGTCCTGCTTTCATCGCTGGTGGGT
GCTGTGGGGGGAGTGATTTTACTGCGCCTGAAAGATACCAACACCTCGACGCCGATCCCCTTTGGACCCTATCTGGCAAT
TGCCGGCTGGATTGCCTTGCTCTGGGGTGGTCAAATAACCGGCTTCTATTGGCAGTTTGTCGGTTTCAAATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (136-244)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.873	97.931	0.528
pilD	Vibrio campbellii strain DS40M4	51.773	97.241	0.503
pilD	Acinetobacter baumannii D1279779	50	97.931	0.49
pilD	Acinetobacter nosocomialis M2	50	97.931	0.49
pilD	Neisseria gonorrhoeae MS11	50.196	87.931	0.441