Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AO353_RS14330 Genome accession   NZ_CP012830
Coordinates   3175522..3177222 (-) Length   566 a.a.
NCBI ID   WP_054595547.1    Uniprot ID   A0A2N8BWI4
Organism   Pseudomonas fluorescens strain FW300-N2E3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3170522..3182222
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO353_RS14295 (AO353_14295) - 3170571..3171503 (+) 933 WP_054595540.1 LysR family transcriptional regulator -
  AO353_RS14300 (AO353_14300) - 3171634..3172320 (+) 687 WP_054595541.1 energy-coupling factor ABC transporter permease -
  AO353_RS14305 (AO353_14305) - 3172384..3172599 (+) 216 WP_054595542.1 hypothetical protein -
  AO353_RS14310 (AO353_14310) yacG 3172609..3172809 (-) 201 WP_054595543.1 DNA gyrase inhibitor YacG -
  AO353_RS14315 (AO353_14315) coaE 3172806..3173429 (-) 624 WP_054595544.1 dephospho-CoA kinase -
  AO353_RS14320 (AO353_14320) pilD 3173426..3174298 (-) 873 WP_054595545.1 prepilin peptidase Machinery gene
  AO353_RS14325 (AO353_14325) pilC 3174301..3175518 (-) 1218 WP_054595546.1 type II secretion system F family protein Machinery gene
  AO353_RS14330 (AO353_14330) pilB 3175522..3177222 (-) 1701 WP_054595547.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AO353_RS14335 (AO353_14335) - 3177449..3177859 (+) 411 WP_054595548.1 pilin -
  AO353_RS14340 (AO353_14340) - 3178137..3178493 (+) 357 WP_054595549.1 BON domain-containing protein -
  AO353_RS14345 (AO353_14345) - 3178605..3178904 (-) 300 WP_054595550.1 DUF2845 domain-containing protein -
  AO353_RS14350 (AO353_14350) - 3178907..3181849 (-) 2943 WP_054595551.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62691.58 Da        Isoelectric Point: 5.8482

>NTDB_id=157789 AO353_RS14330 WP_054595547.1 3175522..3177222(-) (pilB) [Pseudomonas fluorescens strain FW300-N2E3]
MNDIALSGLAKQLVLAELLTDKSAQQAYQQAQRNRVSLVSYLVQNKMVSSRQVAEIASEHFGVAFLDLNCLDKDSQPKDL
VSEKLIRQHHALPLWRRGNKLFVGISDPSNQQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFDSHSTGLEDMADIDLGG
PDIEAVDDQRQDAVTGQDSDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRVRTDGMLREVARPPTQLANRIAARL
KVMASLDISERRKPQDGRLKMHLSKSKSIDFRVNTLPTLWGEKIVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATAVHLIIAQRLARKLCSHCKKNLSIPDETLIKEGFPR
ERIGSFTIYEPVGCDLCNNGYKGRVGIYEVVKNTPQLQRLIMAEGNSLEIDTQMRKDGFNDLRTSGLLKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=157789 AO353_RS14330 WP_054595547.1 3175522..3177222(-) (pilB) [Pseudomonas fluorescens strain FW300-N2E3]
ATGAATGACATCGCCCTCAGCGGTCTGGCCAAGCAATTGGTGCTGGCCGAACTGCTCACTGACAAAAGCGCGCAACAGGC
GTATCAGCAAGCTCAGCGCAATCGCGTTTCCCTGGTCAGCTATCTGGTACAGAACAAAATGGTGAGCAGTCGCCAGGTTG
CGGAAATTGCCTCAGAGCATTTCGGCGTCGCCTTCCTCGACCTCAACTGTCTCGACAAAGACTCCCAGCCCAAAGACCTG
GTCAGTGAAAAGCTGATTCGTCAGCACCACGCCCTGCCACTCTGGCGGCGCGGCAACAAACTGTTCGTGGGCATTTCCGA
CCCGAGCAATCAGCAGGCGATCAACGATATCCAGTTCAGCACCGGGCTCACCACCGAAGCGATCCTGGTCGAAGACGACA
AGCTCAGCGACGCCATCGAGAAATTCTTCGACAGCCACAGCACGGGCCTGGAAGACATGGCCGATATCGACCTCGGGGGC
CCGGACATCGAAGCCGTCGACGACCAGCGCCAGGACGCTGTCACCGGCCAGGACTCCGACGATGCCCCGGTGGTGCGCTT
CGTCAACAAGATGCTGCTAGACGCGATCAAGGGCGGCTCCTCGGACCTGCATTTCGAGCCCTACGAAAAAATCTATCGGG
TGCGGGTACGGACCGACGGCATGCTGCGCGAAGTTGCCAGGCCGCCGACTCAATTGGCCAACCGCATCGCTGCCCGTCTC
AAGGTGATGGCCAGCCTGGACATTTCTGAACGTCGAAAACCCCAGGACGGGCGGCTCAAGATGCACCTGTCGAAAAGCAA
ATCCATCGATTTTCGGGTCAACACCCTGCCAACGTTGTGGGGCGAAAAAATCGTGATCCGGATTCTCGACCCCTCCAGTG
CACAGATGGGCATCGACGCGCTGGGTTACGAGCCCGATCAAAAAGACCTGTATATGGCGGCGCTCAAGCAACCTCAAGGG
CTGATTCTGGTCACCGGGCCGACGGGTTCAGGGAAAACCGTGTCGCTGTACACCGGGTTGAATATCCTTAATACCGTCGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAGGCAGG
GCCTGGATTTCGCTCAGGCGTTGCGCTCATTTCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAAACCGCCGAAATCGCGATCAAGGCTGCGCAAACCGGACACCTGGTGCTTTCCACCCTGCACACCAACAGCGCGGCTGA
AACCCTGACCCGGCTGCACAACATGGGCATCCCGGGGTTCAACATCGCCACGGCCGTGCACCTGATCATTGCCCAGCGCC
TGGCCCGCAAGCTGTGCAGCCACTGCAAAAAAAACCTCTCGATTCCCGACGAAACCCTGATCAAGGAAGGCTTCCCACGG
GAACGCATCGGCTCCTTCACGATCTACGAACCCGTCGGTTGCGATCTCTGCAACAACGGCTACAAAGGGCGAGTCGGAAT
CTATGAAGTGGTGAAAAACACCCCGCAGCTGCAACGGCTGATCATGGCTGAAGGCAATTCTCTGGAAATCGATACGCAAA
TGCGCAAGGACGGCTTCAACGACCTGCGCACCTCGGGCTTGCTCAAGGCCATGCAGGGCATCACCAGCCTCGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N8BWI4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.23

99.823

0.581
  pilB Acinetobacter baumannii D1279779

57.848

100

0.58
  pilB Legionella pneumophila strain ERS1305867

56.36

100

0.564
  pilB Vibrio cholerae strain A1552

49.206

100

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

49.731

98.41

0.489
  pilB Vibrio campbellii strain DS40M4

47.895

100

0.482
  pilF Neisseria gonorrhoeae MS11

47.425

99.47

0.472
  pilF Thermus thermophilus HB27

36.572

100

0.366
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.163

92.933

0.364

Multiple sequence alignment