Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGC   Type   Machinery gene
Locus tag   ACH32_RS00560 Genome accession   NZ_CP012756
Coordinates   105346..105657 (-) Length   103 a.a.
NCBI ID   WP_060649583.1    Uniprot ID   -
Organism   Staphylococcus aureus strain JS395     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 100346..110657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH32_RS00525 (ACH32_00520) gcvPA 100848..102194 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  ACH32_RS00530 (ACH32_00525) gcvT 102214..103305 (-) 1092 WP_000093340.1 glycine cleavage system aminomethyltransferase GcvT -
  ACH32_RS00535 (ACH32_00530) - 103464..103988 (-) 525 WP_001015120.1 shikimate kinase -
  ACH32_RS00540 (ACH32_00535) - 103978..104124 (-) 147 WP_001789879.1 hypothetical protein -
  ACH32_RS00545 (ACH32_00540) comGF 104221..104718 (-) 498 WP_001796472.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACH32_RS00550 (ACH32_00545) comGE 104636..104935 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  ACH32_RS00555 (ACH32_00550) comGD 104922..105368 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACH32_RS00560 (ACH32_00555) comGC 105346..105657 (-) 312 WP_060649583.1 competence type IV pilus major pilin ComGC Machinery gene
  ACH32_RS00565 (ACH32_00560) comGB 105671..106741 (-) 1071 WP_000776414.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACH32_RS00570 (ACH32_00565) comGA 106713..107687 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  ACH32_RS00575 (ACH32_00570) - 107739..108362 (-) 624 WP_001223009.1 MBL fold metallo-hydrolase -
  ACH32_RS00580 (ACH32_00575) - 108359..108688 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ACH32_RS00585 (ACH32_00580) - 108688..109674 (-) 987 WP_000161307.1 ROK family glucokinase -
  ACH32_RS00590 (ACH32_00585) - 109671..109874 (-) 204 WP_000087561.1 YqgQ family protein -

Sequence


Protein


Download         Length: 103 a.a.        Molecular weight: 11284.34 Da        Isoelectric Point: 8.5268

>NTDB_id=157597 ACH32_RS00560 WP_060649583.1 105346..105657(-) (comGC) [Staphylococcus aureus strain JS395]
MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQSTGCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFI
KEAPKTCKSGETITISNGEAVAN

Nucleotide


Download         Length: 312 bp        

>NTDB_id=157597 ACH32_RS00560 WP_060649583.1 105346..105657(-) (comGC) [Staphylococcus aureus strain JS395]
ATGTTTAAATTTCTAAAGAAAACTCAAGCGTTTACATTGATAGAGATGCTATTAGTGTTATTAATCATCAGTTTATTATT
AATTTTAATCATTCCAAATATTGCTAAACAAACTGCTCACATACAATCAACAGGTTGTAATGCACAGGTAAAAATGGTTA
ATAGTCAAATTGAAGCGTATGCATTGAAACATAATAGAAATCCATCGTCTATTGAAGACTTAATTGCAGATGGTTTTATA
AAAGAAGCACCAAAGACATGTAAATCAGGAGAGACAATAACAATTAGTAATGGAGAAGCAGTTGCAAATTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Staphylococcus aureus MW2

99.029

100

0.99

  comGC Staphylococcus aureus N315

99.029

100

0.99

  comGC/cglC Streptococcus pneumoniae TIGR4

45.098

99.029

0.447

  comGC/cglC Streptococcus mitis NCTC 12261

51.19

81.553

0.417

  comGC/cglC Streptococcus mitis SK321

51.19

81.553

0.417

  comGC/cglC Streptococcus pneumoniae R6

50

81.553

0.408

  comGC/cglC Streptococcus pneumoniae Rx1

50

81.553

0.408

  comGC/cglC Streptococcus pneumoniae D39

50

81.553

0.408

  comYC Streptococcus gordonii str. Challis substr. CH1

41.837

95.146

0.398

  comYC Streptococcus suis isolate S10

50

73.786

0.369

  comGC Bacillus subtilis subsp. subtilis str. 168

41.758

88.35

0.369


Multiple sequence alignment