Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   ACH32_RS00545 Genome accession   NZ_CP012756
Coordinates   104221..104718 (-) Length   165 a.a.
NCBI ID   WP_001796472.1    Uniprot ID   A0A5F0HJ55
Organism   Staphylococcus aureus strain JS395     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 99221..109718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH32_RS00520 (ACH32_00515) gcvPB 99383..100855 (-) 1473 WP_000202176.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  ACH32_RS00525 (ACH32_00520) gcvPA 100848..102194 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  ACH32_RS00530 (ACH32_00525) gcvT 102214..103305 (-) 1092 WP_000093340.1 glycine cleavage system aminomethyltransferase GcvT -
  ACH32_RS00535 (ACH32_00530) - 103464..103988 (-) 525 WP_001015120.1 shikimate kinase -
  ACH32_RS00540 (ACH32_00535) - 103978..104124 (-) 147 WP_001789879.1 hypothetical protein -
  ACH32_RS00545 (ACH32_00540) comGF 104221..104718 (-) 498 WP_001796472.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACH32_RS00550 (ACH32_00545) comGE 104636..104935 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  ACH32_RS00555 (ACH32_00550) comGD 104922..105368 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACH32_RS00560 (ACH32_00555) comGC 105346..105657 (-) 312 WP_060649583.1 competence type IV pilus major pilin ComGC Machinery gene
  ACH32_RS00565 (ACH32_00560) comGB 105671..106741 (-) 1071 WP_000776414.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACH32_RS00570 (ACH32_00565) comGA 106713..107687 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  ACH32_RS00575 (ACH32_00570) - 107739..108362 (-) 624 WP_001223009.1 MBL fold metallo-hydrolase -
  ACH32_RS00580 (ACH32_00575) - 108359..108688 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ACH32_RS00585 (ACH32_00580) - 108688..109674 (-) 987 WP_000161307.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19169.89 Da        Isoelectric Point: 10.1358

>NTDB_id=157594 ACH32_RS00545 WP_001796472.1 104221..104718(-) (comGF) [Staphylococcus aureus strain JS395]
MILSKVTNKYVPFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=157594 ACH32_RS00545 WP_001796472.1 104221..104718(-) (comGF) [Staphylococcus aureus strain JS395]
ATGATATTAAGCAAAGTGACCAACAAATATGTGCCATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5F0HJ55

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

95.758

100

0.958

Multiple sequence alignment