Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACH32_RS00570 Genome accession   NZ_CP012756
Coordinates   106713..107687 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain JS395     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 101713..112687
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH32_RS00530 (ACH32_00525) gcvT 102214..103305 (-) 1092 WP_000093340.1 glycine cleavage system aminomethyltransferase GcvT -
  ACH32_RS00535 (ACH32_00530) - 103464..103988 (-) 525 WP_001015120.1 shikimate kinase -
  ACH32_RS00540 (ACH32_00535) - 103978..104124 (-) 147 WP_001789879.1 hypothetical protein -
  ACH32_RS00545 (ACH32_00540) comGF 104221..104718 (-) 498 WP_001796472.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACH32_RS00550 (ACH32_00545) comGE 104636..104935 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  ACH32_RS00555 (ACH32_00550) comGD 104922..105368 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACH32_RS00560 (ACH32_00555) comGC 105346..105657 (-) 312 WP_060649583.1 competence type IV pilus major pilin ComGC Machinery gene
  ACH32_RS00565 (ACH32_00560) comGB 105671..106741 (-) 1071 WP_000776414.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACH32_RS00570 (ACH32_00565) comGA 106713..107687 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  ACH32_RS00575 (ACH32_00570) - 107739..108362 (-) 624 WP_001223009.1 MBL fold metallo-hydrolase -
  ACH32_RS00580 (ACH32_00575) - 108359..108688 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ACH32_RS00585 (ACH32_00580) - 108688..109674 (-) 987 WP_000161307.1 ROK family glucokinase -
  ACH32_RS00590 (ACH32_00585) - 109671..109874 (-) 204 WP_000087561.1 YqgQ family protein -
  ACH32_RS00595 (ACH32_00590) - 109855..111318 (-) 1464 WP_060649584.1 rhomboid family protein -
  ACH32_RS00600 (ACH32_00595) - 111330..111869 (-) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  ACH32_RS00605 (ACH32_00600) rpmG 112078..112227 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=157599 ACH32_RS00570 WP_000697220.1 106713..107687(-) (comGA) [Staphylococcus aureus strain JS395]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=157599 ACH32_RS00570 WP_000697220.1 106713..107687(-) (comGA) [Staphylococcus aureus strain JS395]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGACTTGATGTATCTACACAACAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTAATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATTGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1

Multiple sequence alignment