Detailed information NTG0156020

Bioinformatically predicted

Overview

• Name: comE/blpR
• Type: Regulator
• Locus tag: RN80_RS05125
• Genome accession: NZ_CP012646
• Coordinates: 1049453..1050193 (+)
• Length: 246 a.a.
• NCBI ID: WP_060627927.1
• Uniprot ID: A0A1E9GHA2
• Organism: Streptococcus mitis strain KCOM 1350 (= ChDC B183)
• Function: activate transcription of early competence genes; regulation of comX expression (predicted from homology) Competence regulation

Genomic Context

Location: 1044453..1055193

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RN80_RS05105 (RN80_05125)	-	1045848..1046663 (+)	816	WP_060627920.1	endonuclease/exonuclease/phosphatase family protein	-
RN80_RS05110 (RN80_05130)	-	1046910..1048253 (+)	1344	WP_060627923.1	DEAD/DEAH box helicase	-
RN80_RS05115	-	1048511..1048909 (-)	399	WP_060627924.1	hypothetical protein	-
RN80_RS05120 (RN80_05140)	-	1049110..1049442 (+)	333	WP_060627925.1	LytTR family DNA-binding domain-containing protein	-
RN80_RS05125 (RN80_05145)	comE/blpR	1049453..1050193 (+)	741	WP_060627927.1	response regulator transcription factor	Regulator
RN80_RS05130 (RN80_05150)	-	1050204..1051529 (+)	1326	WP_060627929.1	GHKL domain-containing protein	-
RN80_RS09915	-	1052483..1052653 (+)	171	WP_125393441.1	bacteriocin secretion protein	-
RN80_RS05140 (RN80_05160)	-	1053007..1053306 (+)	300	WP_045607191.1	bacteriocin immunity protein	-
RN80_RS05145 (RN80_05165)	metK	1053948..1055138 (+)	1191	WP_060627931.1	methionine adenosyltransferase	-

Sequence

Protein

• Length: 246 a.a.
• Molecular weight: 28610.82 Da
• Isoelectric Point: 5.6447

>NTDB_id=156020 RN80_RS05125 WP_060627927.1 1049453..1050193(+) (comE/blpR) [Streptococcus mitis strain KCOM 1350 (= ChDC B183)]
MNIFILEDNFLQQTRIENVVKKILVDNKIEYRHFEVYGKPQQLLEDISERGNHQLFLLDIEIKDADKRGLDVAREIRKLD
SQAVIAFVTSHSEFMPVSFEYLVSAIDFIDKELPEPLFIKRIENVILSVSDNQGITVSEDSFIFTGSKAQIQVPFKDLLY
IETSSIAHKLTLYSKRDIVEFYGQLSEIIEQEPRLFQCHRSFIVNPYNISSIDKENRVVHFQNGSSCIVSRLKIRSLMKA
VERLHD

Nucleotide

• Length: 741 bp

>NTDB_id=156020 RN80_RS05125 WP_060627927.1 1049453..1050193(+) (comE/blpR) [Streptococcus mitis strain KCOM 1350 (= ChDC B183)]
GTGAATATTTTTATACTAGAAGATAATTTTTTACAACAGACTAGAATTGAAAATGTTGTCAAAAAAATTTTGGTGGATAA
TAAGATTGAGTATAGGCATTTTGAGGTTTATGGAAAACCTCAACAGCTTTTAGAGGACATTTCAGAGAGAGGAAATCATC
AATTATTTTTACTTGATATTGAAATAAAAGATGCTGACAAAAGAGGATTAGACGTTGCTAGAGAAATTAGAAAACTTGAT
TCTCAAGCAGTGATTGCTTTTGTAACATCACATTCTGAGTTTATGCCTGTATCTTTTGAATATTTAGTATCAGCTATTGA
TTTCATTGATAAAGAATTACCTGAACCATTATTTATCAAACGAATTGAAAATGTTATTTTAAGTGTTAGCGATAATCAAG
GAATAACAGTATCTGAGGACTCTTTTATATTTACAGGATCTAAAGCTCAAATTCAAGTCCCTTTTAAAGATTTATTGTAT
ATTGAGACATCGTCAATAGCCCATAAATTGACACTCTATTCAAAACGAGACATAGTTGAATTTTATGGACAATTATCCGA
AATCATAGAACAGGAACCACGCTTATTTCAATGTCATAGATCGTTTATCGTAAATCCTTATAATATTTCTTCAATTGATA
AAGAAAATCGGGTAGTTCATTTTCAAAACGGGTCCTCTTGTATTGTCTCTAGGTTAAAGATACGTTCACTCATGAAAGCT
GTCGAAAGACTTCATGACTAA

Domains

Predicted by InterproScan.

• Response regulator receiver domain (Response_reg) (3-122)

• LytTr DNA-binding domain (LytTR) (151-240)

3D structure

• AlphaFold DB: A0A1E9GHA2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comE/blpR	Streptococcus mutans UA159	51.261	96.748	0.496
comE/comE1	Streptococcus equinus JB1	44.118	96.748	0.427
comE/comE1	Streptococcus gordonii str. Challis substr. CH1	38.017	98.374	0.374
comE/comE2	Streptococcus gordonii strain NCTC7865	38.017	98.374	0.374
comE	Streptococcus mitis SK321	36.4	100	0.37
comE	Streptococcus mitis NCTC 12261	36.4	100	0.37
comE	Streptococcus pneumoniae TIGR4	36	100	0.366
comE	Streptococcus pneumoniae Rx1	36	100	0.366
comE	Streptococcus pneumoniae D39	36	100	0.366
comE	Streptococcus pneumoniae R6	36	100	0.366
comE	Streptococcus infantis strain Atu-4	36	100	0.366
comE/comE2	Streptococcus equinus JB1	36.475	99.187	0.362