Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbB/cilA   Type   Machinery gene
Locus tag   RN80_RS00035 Genome accession   NZ_CP012646
Coordinates   5354..5749 (-) Length   131 a.a.
NCBI ID   WP_060627102.1    Uniprot ID   A0A0M4JTN8
Organism   Streptococcus mitis strain KCOM 1350 (= ChDC B183)     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context

Location: 354..10749
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RN80_RS00010 (RN80_00010) recX 903..1679 (+) 777 WP_060627099.1 recombination regulator RecX -
  RN80_RS00015 (RN80_00015) - 1768..2301 (+) 534 WP_000775316.1 DUF402 domain-containing protein -
  RN80_RS00020 (RN80_00020) - 2371..3138 (-) 768 WP_060627100.1 epoxyqueuosine reductase QueH -
  RN80_RS00025 (RN80_00025) groL 3276..4898 (-) 1623 WP_060627101.1 chaperonin GroEL -
  RN80_RS00030 (RN80_00030) groES 4914..5198 (-) 285 WP_000917335.1 co-chaperone GroES -
  RN80_RS00035 (RN80_00035) ssbB/cilA 5354..5749 (-) 396 WP_060627102.1 single-stranded DNA-binding protein Machinery gene
  RN80_RS00040 (RN80_00040) - 5826..6587 (-) 762 WP_060627103.1 SDR family NAD(P)-dependent oxidoreductase -
  RN80_RS00045 (RN80_00045) ytpR 6629..7255 (-) 627 WP_060627104.1 YtpR family tRNA-binding protein -
  RN80_RS00050 (RN80_00050) - 7271..7588 (-) 318 WP_023948252.1 thioredoxin family protein -
  RN80_RS00055 (RN80_00055) - 7585..7884 (-) 300 WP_050243274.1 DUF4651 domain-containing protein -
  RN80_RS00060 (RN80_00060) - 8076..9149 (-) 1074 WP_060627105.1 N-acetylmuramoyl-L-alanine amidase family protein -

Sequence

Protein


Download         Length: 131 a.a.        Molecular weight: 14921.88 Da        Isoelectric Point: 5.9409

>NTDB_id=155961 RN80_RS00035 WP_060627102.1 5354..5749(-) (ssbB/cilA) [Streptococcus mitis strain KCOM 1350 (= ChDC B183)]
MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGEREVDFVNMVLWGRLAESLASYATKGSLISVDGELRTR
RFEKNGQMNYVTEVLVTGFQLLESRAQRAMRENNAGQDLADLVLEEEELPF

Nucleotide


Download         Length: 396 bp        

>NTDB_id=155961 RN80_RS00035 WP_060627102.1 5354..5749(-) (ssbB/cilA) [Streptococcus mitis strain KCOM 1350 (= ChDC B183)]
ATGTATAATAAAGTTATCTTAATCGGACGCTTGACGTCTACACCAGAATTGCACAAAACCAACAATGACAAGTCAGTAGC
GCGAGCAACTATCGCTGTAAATCGTCGTTACAAAGACCAAAATGGGGAGCGCGAAGTTGATTTTGTTAATATGGTCCTAT
GGGGCAGACTAGCAGAAAGCTTGGCAAGCTACGCAACCAAAGGTAGTCTTATTTCAGTGGATGGAGAATTACGTACCCGT
CGTTTTGAGAAAAATGGTCAGATGAACTACGTGACCGAAGTACTTGTTACTGGATTCCAACTCTTGGAAAGTCGTGCTCA
ACGTGCCATGCGTGAAAATAATGCAGGACAAGATTTGGCGGATTTGGTTTTGGAAGAGGAAGAATTGCCATTTTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(3-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0M4JTN8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbB/cilA Streptococcus pneumoniae TIGR4

98.473

100

0.985
  ssbB/cilA Streptococcus mitis NCTC 12261

97.71

100

0.977
  ssbB/cilA Streptococcus pneumoniae Rx1

97.71

100

0.977
  ssbB/cilA Streptococcus pneumoniae D39

97.71

100

0.977
  ssbB/cilA Streptococcus pneumoniae R6

97.71

100

0.977
  ssbB/cilA Streptococcus mitis SK321

97.71

100

0.977
  ssbA Streptococcus mutans UA159

74.046

100

0.74
  ssbB Streptococcus sobrinus strain NIDR 6715-7

70.992

100

0.71
  ssbB Lactococcus lactis subsp. cremoris KW2

58.929

85.496

0.504
  ssbA Bacillus subtilis subsp. subtilis str. 168

50.943

80.916

0.412
  ssb Latilactobacillus sakei subsp. sakei 23K

46.903

86.26

0.405

Multiple sequence alignment