Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   A8L40_RS04355 Genome accession   NZ_CP015831
Coordinates   845691..846350 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli O157 strain 644-PT8     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 840691..851350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8L40_RS04330 - 841433..842380 (+) 948 WP_001301797.1 iron-siderophore ABC transporter substrate-binding protein -
  A8L40_RS04335 - 842377..843264 (+) 888 WP_000614950.1 MurR/RpiR family transcriptional regulator -
  A8L40_RS04340 ygiN 843309..843623 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  A8L40_RS04345 mdaB 843654..844235 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  A8L40_RS04350 qseC 844345..845694 (-) 1350 WP_000673362.1 quorum sensing histidine kinase QseC -
  A8L40_RS04355 ciaR 845691..846350 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  A8L40_RS04360 ygiW 846502..846894 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  A8L40_RS04365 ygiV 846947..847429 (+) 483 WP_000183479.1 GyrI-like domain-containing protein -
  A8L40_RS04370 ygiS 847538..849145 (+) 1608 WP_001301989.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=154779 A8L40_RS04355 WP_001221502.1 845691..846350(-) (ciaR) [Escherichia coli O157 strain 644-PT8]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=154779 A8L40_RS04355 WP_001221502.1 845691..846350(-) (ciaR) [Escherichia coli O157 strain 644-PT8]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment