Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AKN90_RS05290 Genome accession   NZ_CP012363
Coordinates   1142098..1143315 (-) Length   405 a.a.
NCBI ID   WP_053106021.1    Uniprot ID   -
Organism   Thiopseudomonas alkaliphila strain E1086     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1137098..1148315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AKN90_RS05265 (AKN90_05275) - 1137621..1138079 (+) 459 WP_053103346.1 YiiD C-terminal domain-containing protein -
  AKN90_RS05270 (AKN90_05280) - 1138076..1139287 (+) 1212 WP_053106018.1 NAD(P)/FAD-dependent oxidoreductase -
  AKN90_RS05275 (AKN90_05285) - 1139454..1140443 (+) 990 WP_053105048.1 hypothetical protein -
  AKN90_RS05280 (AKN90_05290) - 1140708..1140998 (+) 291 WP_053106019.1 GIY-YIG nuclease family protein -
  AKN90_RS05285 (AKN90_05295) pilD 1141195..1142094 (-) 900 WP_053106020.1 prepilin peptidase Machinery gene
  AKN90_RS05290 (AKN90_05300) pilC 1142098..1143315 (-) 1218 WP_053106021.1 type II secretion system F family protein Machinery gene
  AKN90_RS05295 (AKN90_05305) pilB 1143321..1145012 (-) 1692 WP_053106022.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AKN90_RS05300 (AKN90_05310) - 1145283..1145792 (+) 510 WP_053105043.1 pilin -
  AKN90_RS05305 (AKN90_05315) elbB 1145863..1146516 (-) 654 WP_053106023.1 isoprenoid biosynthesis glyoxalase ElbB -
  AKN90_RS05310 (AKN90_05320) - 1146559..1146975 (-) 417 WP_053106024.1 PACE efflux transporter -
  AKN90_RS05315 (AKN90_05325) rplQ 1147054..1147437 (-) 384 WP_053100999.1 50S ribosomal protein L17 -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44100.92 Da        Isoelectric Point: 9.7844

>NTDB_id=153808 AKN90_RS05290 WP_053106021.1 1142098..1143315(-) (pilC) [Thiopseudomonas alkaliphila strain E1086]
MEIKKSPKSYIYVWHGKSRQGNKLSGVINSHDINIVKAQLRKQGVLHAKVKKKGITFGRDKKIKSMDITIFTRQMATMMQ
AGVPLLQSFTIIIEGLDNSNMRKLLEAVRNDIAGGSSFAGALRKHSLYFDELYCNLVASGETSGALETLLDRVATYKEKS
EALKSKIKKAMNYPIAVVVVAAVVTSVLLLKVVPQFQEIFEGFGAELPVFTQLVIGISELLQEYSLYVLIGLVLLLYAAR
RTLRRSEQLRNRADKILLKMPIIGDILYNSAVARYGRTLATTFSAGVPLVDALDSVSGAIGNVVFKSAVQQVKSDVSTGM
QLHTSMMSSGVFPPLALQLTAIGEESGALDTMLEKVAAYYEAEVDNAVDGLTALLEPIIMVVLGVLIGGLVIAMYLPILQ
MGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=153808 AKN90_RS05290 WP_053106021.1 1142098..1143315(-) (pilC) [Thiopseudomonas alkaliphila strain E1086]
ATGGAAATTAAGAAGTCGCCTAAAAGCTATATATATGTTTGGCATGGTAAAAGTAGGCAAGGGAATAAGCTAAGTGGTGT
TATTAATTCGCATGATATTAATATTGTAAAGGCTCAGCTAAGAAAGCAGGGAGTTCTGCATGCTAAGGTGAAAAAGAAAG
GTATCACCTTTGGCCGCGATAAAAAAATTAAATCCATGGACATAACCATCTTTACTCGGCAAATGGCCACCATGATGCAA
GCCGGGGTGCCTTTATTACAGTCGTTTACCATTATTATTGAAGGCTTAGACAATTCCAATATGCGTAAATTACTAGAAGC
TGTGCGTAATGATATTGCTGGGGGTAGCAGTTTTGCAGGAGCTTTACGCAAACACTCACTATATTTTGATGAGTTGTACT
GCAATTTAGTGGCCTCAGGCGAAACGTCGGGAGCTTTGGAAACTTTGCTTGATCGAGTAGCGACCTATAAAGAAAAGTCA
GAGGCGCTTAAATCTAAAATTAAAAAAGCCATGAACTACCCGATTGCTGTAGTGGTGGTGGCAGCTGTTGTGACGAGTGT
TTTGCTATTAAAAGTGGTACCACAGTTCCAGGAGATCTTTGAAGGTTTTGGAGCTGAGTTGCCTGTATTTACGCAATTGG
TAATTGGTATTTCAGAGTTGCTGCAAGAGTATAGTTTATATGTGCTGATAGGCTTAGTACTGTTACTTTATGCTGCTCGT
AGGACGTTGCGCCGATCAGAGCAGTTGCGCAATCGTGCTGATAAAATACTGCTAAAAATGCCGATAATAGGTGATATTTT
ATATAACTCAGCTGTGGCTCGTTATGGGCGCACCTTAGCTACAACCTTCTCAGCCGGCGTACCTTTGGTTGATGCGTTAG
ATTCAGTTTCTGGCGCAATCGGCAACGTGGTTTTTAAAAGTGCAGTGCAGCAAGTGAAGTCCGATGTGTCTACTGGTATG
CAGTTGCATACTTCAATGATGTCTAGCGGGGTATTTCCTCCCCTAGCACTACAACTTACTGCTATTGGTGAAGAGTCTGG
TGCGCTAGATACCATGTTAGAAAAAGTTGCAGCGTACTATGAAGCGGAAGTAGATAACGCTGTGGATGGCTTAACTGCGT
TGCTAGAGCCAATTATTATGGTGGTGCTAGGTGTACTAATTGGCGGACTAGTTATTGCCATGTACCTGCCAATCTTACAA
ATGGGTAACGTGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(277-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

65.012

99.506

0.647
  pilC Acinetobacter baumannii D1279779

54.657

100

0.551
  pilC Acinetobacter baylyi ADP1

53.659

100

0.543
  pilC Legionella pneumophila strain ERS1305867

49.01

99.753

0.489
  pilG Neisseria gonorrhoeae MS11

43.291

97.531

0.422
  pilG Neisseria meningitidis 44/76-A

43.038

97.531

0.42
  pilC Vibrio campbellii strain DS40M4

42.04

99.259

0.417
  pilC Vibrio cholerae strain A1552

41.162

97.778

0.402
  pilC Thermus thermophilus HB27

39.85

98.519

0.393

Multiple sequence alignment