Detailed information NTG0153807

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AKN90_RS05285
• Genome accession: NZ_CP012363
• Coordinates: 1141195..1142094 (-)
• Length: 299 a.a.
• NCBI ID: WP_053106020.1
• Uniprot ID: -
• Organism: Thiopseudomonas alkaliphila strain E1086
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1136195..1147094

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AKN90_RS05265 (AKN90_05275)	-	1137621..1138079 (+)	459	WP_053103346.1	YiiD C-terminal domain-containing protein	-
AKN90_RS05270 (AKN90_05280)	-	1138076..1139287 (+)	1212	WP_053106018.1	NAD(P)/FAD-dependent oxidoreductase	-
AKN90_RS05275 (AKN90_05285)	-	1139454..1140443 (+)	990	WP_053105048.1	hypothetical protein	-
AKN90_RS05280 (AKN90_05290)	-	1140708..1140998 (+)	291	WP_053106019.1	GIY-YIG nuclease family protein	-
AKN90_RS05285 (AKN90_05295)	pilD	1141195..1142094 (-)	900	WP_053106020.1	prepilin peptidase	Machinery gene
AKN90_RS05290 (AKN90_05300)	pilC	1142098..1143315 (-)	1218	WP_053106021.1	type II secretion system F family protein	Machinery gene
AKN90_RS05295 (AKN90_05305)	pilB	1143321..1145012 (-)	1692	WP_053106022.1	type IV-A pilus assembly ATPase PilB	Machinery gene
AKN90_RS05300 (AKN90_05310)	-	1145283..1145792 (+)	510	WP_053105043.1	pilin	-
AKN90_RS05305 (AKN90_05315)	elbB	1145863..1146516 (-)	654	WP_053106023.1	isoprenoid biosynthesis glyoxalase ElbB	-
AKN90_RS05310 (AKN90_05320)	-	1146559..1146975 (-)	417	WP_053106024.1	PACE efflux transporter	-

Sequence

Protein

• Length: 299 a.a.
• Molecular weight: 32764.31 Da
• Isoelectric Point: 8.1041

>NTDB_id=153807 AKN90_RS05285 WP_053106020.1 1141195..1142094(-) (pilD) [Thiopseudomonas alkaliphila strain E1086]
MSDLLGLLQQELALFIALASLLGLLAGSFINVVAYRLPKMLQADWQQQAKVILAEAQPAVAVRPEPDSTSPRFNLLVPRS
ACPRCQHVIKLWENIPLLSWLALKGKCSGCQQIISKRYPLVELASGILSAVVAWRFGFGLPAMAILLLTWGLLTMSLIDA
DHQLLPDILVLPLLWLGLLLNTQSMFVSLSDAVLGVAIGYLSLWSIYWLFKLIMGKEGMGYGDFKLLAMLGAWGGWQVLL
LTILLSSLVGAILGIIILRLQKEGIEKPISYGPYLAVAGWIALLWGNQITQAYLEFAGL

Nucleotide

• Length: 900 bp

>NTDB_id=153807 AKN90_RS05285 WP_053106020.1 1141195..1142094(-) (pilD) [Thiopseudomonas alkaliphila strain E1086]
ATGAGTGATTTGCTTGGGTTACTACAGCAAGAGTTAGCTTTATTTATAGCTCTGGCTTCACTTTTAGGGTTATTAGCTGG
CAGCTTTATCAATGTGGTGGCATATCGCCTACCAAAAATGCTGCAGGCCGACTGGCAACAACAAGCTAAAGTAATTCTAG
CCGAAGCCCAGCCAGCGGTAGCTGTACGCCCAGAGCCTGATTCTACGAGTCCACGTTTTAACTTATTGGTACCACGCTCA
GCCTGCCCCCGTTGCCAGCATGTGATTAAGCTTTGGGAGAATATTCCGTTGCTTAGTTGGCTGGCGCTGAAAGGTAAGTG
CTCAGGCTGTCAGCAAATCATTAGTAAGCGCTATCCCTTGGTGGAGCTGGCCAGCGGCATTTTATCGGCAGTAGTGGCCT
GGAGATTTGGCTTTGGCCTACCAGCTATGGCTATATTGCTATTGACCTGGGGGCTACTGACGATGAGCCTGATTGACGCT
GATCATCAACTGCTTCCGGATATTTTAGTGTTGCCGTTACTTTGGCTGGGCTTGCTGCTAAATACCCAGTCTATGTTTGT
CAGCTTAAGTGATGCGGTATTGGGAGTAGCTATCGGTTATCTAAGTCTATGGAGTATTTATTGGCTATTTAAGCTAATTA
TGGGCAAAGAAGGCATGGGTTACGGTGACTTTAAGTTGCTGGCGATGCTGGGCGCTTGGGGTGGCTGGCAAGTCTTATTG
CTAACAATTTTACTGTCTTCGCTGGTGGGCGCAATTTTAGGCATTATTATTTTGCGCTTACAAAAAGAAGGCATAGAAAA
GCCTATTTCCTATGGCCCTTACCTCGCCGTCGCCGGATGGATCGCCTTACTATGGGGCAACCAAATCACCCAAGCTTATT
TGGAGTTTGCGGGGCTCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (147-255)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-136)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.71	94.649	0.508
pilD	Acinetobacter baumannii D1279779	49.492	98.662	0.488
pilD	Acinetobacter nosocomialis M2	48.814	98.662	0.482
pilD	Vibrio campbellii strain DS40M4	50.355	94.314	0.475
pilD	Neisseria gonorrhoeae MS11	45.126	92.642	0.418