Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AKN90_RS05295 Genome accession   NZ_CP012363
Coordinates   1143321..1145012 (-) Length   563 a.a.
NCBI ID   WP_053106022.1    Uniprot ID   -
Organism   Thiopseudomonas alkaliphila strain E1086     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1138321..1150012
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AKN90_RS05275 (AKN90_05285) - 1139454..1140443 (+) 990 WP_053105048.1 hypothetical protein -
  AKN90_RS05280 (AKN90_05290) - 1140708..1140998 (+) 291 WP_053106019.1 GIY-YIG nuclease family protein -
  AKN90_RS05285 (AKN90_05295) pilD 1141195..1142094 (-) 900 WP_053106020.1 prepilin peptidase Machinery gene
  AKN90_RS05290 (AKN90_05300) pilC 1142098..1143315 (-) 1218 WP_053106021.1 type II secretion system F family protein Machinery gene
  AKN90_RS05295 (AKN90_05305) pilB 1143321..1145012 (-) 1692 WP_053106022.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AKN90_RS05300 (AKN90_05310) - 1145283..1145792 (+) 510 WP_053105043.1 pilin -
  AKN90_RS05305 (AKN90_05315) elbB 1145863..1146516 (-) 654 WP_053106023.1 isoprenoid biosynthesis glyoxalase ElbB -
  AKN90_RS05310 (AKN90_05320) - 1146559..1146975 (-) 417 WP_053106024.1 PACE efflux transporter -
  AKN90_RS05315 (AKN90_05325) rplQ 1147054..1147437 (-) 384 WP_053100999.1 50S ribosomal protein L17 -
  AKN90_RS05320 (AKN90_05330) - 1147481..1148482 (-) 1002 WP_053100998.1 DNA-directed RNA polymerase subunit alpha -
  AKN90_RS05325 (AKN90_05335) rpsD 1148505..1149125 (-) 621 WP_053100997.1 30S ribosomal protein S4 -
  AKN90_RS05330 (AKN90_05340) rpsK 1149144..1149533 (-) 390 WP_053100996.1 30S ribosomal protein S11 -
  AKN90_RS05335 (AKN90_05345) rpsM 1149564..1149920 (-) 357 WP_053100995.1 30S ribosomal protein S13 -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62467.50 Da        Isoelectric Point: 6.2895

>NTDB_id=153809 AKN90_RS05295 WP_053106022.1 1143321..1145012(-) (pilB) [Thiopseudomonas alkaliphila strain E1086]
MELSSGLIGLAGQIVKLGLADYKKVLNSVTDARLAGQPIISWLVHNKIVTSREVMTLATSQFGVASFDLSCFKIKLLSKD
LLSERIVRKHRVLPLAKRGNLLYVALADPGDQLVLNEIKFTTGCAVELVVVEQDKLDLLIEQLYDSLLENFESLADEVLE
ESEYIEIQNELIIDAIDEAPIVKFVNSMLLRAIKIGASDLHFEPYENSYRVRFRTDGILHEVSKQPVGLRDRISTRLKVM
ASIDISERRKPQDGRIKLKVSSVKSINFRVNTLPTLWGEKIVLRILDSDSTKLGIDSLGYESAQKKLYLEALDLPQGMIL
VTGPTGSGKSVSLYTGINIINTVERNISTAEDPVEIYLEGINQVNVNFKQGMDFAQALRAFLRQDPDVIMIGEIRDLETA
EIAIKAAQTGHMVMSTLHTNSAADTLTRLRNMGVAAFNLAISINLIIAQRLARRLCNICKSVKKIPVDILLQEGFSLEQV
GTFKVYEAVGCDKCNAGYKGRIGIYEVVKITPALQAIIMEGGNPIEINQQIRKEGFNDLRTSALEKVIQGVVSLEEVNRV
TKD

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=153809 AKN90_RS05295 WP_053106022.1 1143321..1145012(-) (pilB) [Thiopseudomonas alkaliphila strain E1086]
ATGGAGTTATCAAGCGGCTTAATAGGTCTGGCAGGACAAATTGTCAAATTAGGTTTAGCAGACTATAAAAAGGTTTTAAA
CTCTGTTACAGATGCTAGGCTGGCGGGCCAGCCAATTATTTCTTGGCTAGTGCATAATAAAATTGTCACTTCTCGGGAAG
TGATGACATTAGCTACAAGCCAATTTGGAGTAGCTAGTTTTGATTTAAGCTGTTTTAAAATAAAATTACTGAGTAAAGAT
TTACTTAGTGAAAGAATTGTTCGTAAACACCGAGTATTACCTTTAGCTAAGCGAGGCAATCTGCTTTATGTTGCCTTGGC
AGACCCTGGAGATCAGCTTGTACTTAATGAAATAAAATTTACGACTGGCTGTGCTGTTGAGCTAGTCGTAGTAGAGCAAG
ATAAATTGGATCTGCTTATTGAGCAACTTTATGACAGCTTGCTTGAGAATTTTGAAAGTCTGGCTGATGAAGTCTTAGAA
GAGTCTGAGTATATTGAAATACAAAATGAGCTAATAATAGATGCTATAGATGAGGCTCCTATAGTTAAATTTGTAAATAG
TATGTTATTGCGTGCTATTAAAATAGGTGCTTCAGATTTACATTTTGAACCCTATGAAAATAGTTATAGAGTTCGCTTTC
GTACTGATGGGATATTGCATGAAGTAAGCAAGCAGCCTGTAGGTTTACGTGATCGGATCTCTACACGGCTCAAGGTTATG
GCTAGCATTGATATTTCTGAGCGTCGTAAACCACAAGACGGAAGAATTAAACTAAAGGTATCATCGGTAAAGTCTATAAA
TTTTCGAGTTAATACTTTACCTACTTTATGGGGTGAAAAAATTGTGCTAAGGATTTTAGATTCTGATAGTACAAAACTAG
GAATTGACTCGTTAGGCTATGAGTCAGCACAAAAAAAGCTTTATTTAGAAGCTTTAGATTTACCCCAAGGTATGATTTTA
GTGACTGGGCCTACTGGCTCGGGTAAAAGTGTATCTTTATATACAGGAATCAATATTATCAACACTGTAGAACGTAACAT
TTCTACTGCAGAAGATCCTGTGGAAATCTATTTGGAAGGGATTAATCAAGTTAATGTTAACTTCAAGCAAGGAATGGATT
TTGCACAAGCTTTGCGTGCTTTTTTACGACAGGATCCTGATGTAATAATGATTGGAGAAATTCGTGACTTAGAAACTGCA
GAAATTGCAATTAAGGCGGCACAAACTGGACACATGGTAATGTCAACTTTACATACAAATAGTGCTGCAGACACTCTTAC
ACGTCTACGTAATATGGGAGTAGCAGCCTTTAATTTAGCTATTTCGATTAATTTAATCATTGCCCAACGGTTAGCTAGAC
GTTTATGTAATATATGTAAGTCTGTTAAGAAAATACCAGTTGACATTTTATTGCAGGAGGGTTTTAGCTTAGAGCAAGTA
GGCACTTTTAAGGTATATGAGGCTGTTGGCTGTGATAAATGTAATGCGGGATATAAAGGGCGAATTGGAATTTATGAAGT
GGTGAAAATTACACCTGCATTACAAGCTATTATTATGGAAGGGGGGAATCCTATTGAAATTAACCAACAAATACGTAAAG
AAGGTTTTAATGATCTACGCACTTCAGCGTTAGAGAAGGTAATTCAGGGAGTTGTTAGTTTAGAAGAGGTGAATCGAGTA
ACTAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-454)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.204

99.29

0.538
  pilB Acinetobacter baumannii D1279779

55.617

96.448

0.536
  pilB Legionella pneumophila strain ERS1305867

52.533

94.671

0.497
  pilB Vibrio parahaemolyticus RIMD 2210633

47.292

98.401

0.465
  pilF Neisseria gonorrhoeae MS11

48.785

95.027

0.464
  pilB Vibrio campbellii strain DS40M4

46.085

99.822

0.46
  pilB Vibrio cholerae strain A1552

47.77

95.56

0.456
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.273

97.691

0.364
  pilB/pilB1 Synechocystis sp. PCC 6803

39.038

92.362

0.361

Multiple sequence alignment