Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA2   Type   Machinery gene
Locus tag   WM2015_RS16360 Genome accession   NZ_CP012154
Coordinates   2251955..2252404 (+) Length   149 a.a.
NCBI ID   WP_281173112.1    Uniprot ID   A0A0K0XX46
Organism   Wenzhouxiangella marina strain KCTC 42284     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2244465..2269677 2251955..2252404 within 0


Gene organization within MGE regions


Location: 2244465..2269677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WM2015_RS09530 (WM2015_1879) - 2244465..2246438 (-) 1974 WP_156201043.1 hypothetical protein -
  WM2015_RS09535 (WM2015_1880) - 2246707..2247600 (+) 894 WP_082169626.1 glycosyltransferase family 2 protein -
  WM2015_RS09540 - 2247659..2248663 (-) 1005 WP_156201046.1 hypothetical protein -
  WM2015_RS09545 (WM2015_1882) - 2248615..2249241 (-) 627 WP_049725826.1 class I SAM-dependent methyltransferase -
  WM2015_RS09550 (WM2015_1883) - 2249771..2251681 (+) 1911 WP_049725827.1 hypothetical protein -
  WM2015_RS16360 (WM2015_1884) pilA2 2251955..2252404 (+) 450 WP_281173112.1 pilin Machinery gene
  WM2015_RS16365 (WM2015_1885) - 2252660..2253097 (+) 438 WP_049725829.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  WM2015_RS09565 (WM2015_1886) pilB 2253206..2254930 (+) 1725 WP_049725830.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WM2015_RS09570 (WM2015_1887) pilC 2255170..2256390 (+) 1221 WP_049725831.1 type II secretion system F family protein Machinery gene
  WM2015_RS09575 (WM2015_1888) pilD 2256521..2257342 (+) 822 WP_049727044.1 A24 family peptidase Machinery gene
  WM2015_RS09580 (WM2015_1889) - 2257610..2258311 (-) 702 WP_049725832.1 response regulator transcription factor -
  WM2015_RS09585 (WM2015_1890) - 2258494..2260386 (-) 1893 WP_169751138.1 sulfatase-like hydrolase/transferase -
  WM2015_RS09590 (WM2015_1891) rho 2260519..2261775 (-) 1257 WP_049725834.1 transcription termination factor Rho -
  WM2015_RS09595 (WM2015_1892) trxA 2262042..2262368 (-) 327 WP_049725835.1 thioredoxin TrxA -
  WM2015_RS09600 (WM2015_1893) - 2262584..2263879 (+) 1296 WP_049725836.1 DEAD/DEAH box helicase -
  WM2015_RS09605 (WM2015_1894) - 2263948..2264844 (+) 897 WP_049725837.1 type II secretion system protein N -
  WM2015_RS09610 (WM2015_1895) - 2264841..2266757 (+) 1917 WP_049725838.1 secretin N-terminal domain-containing protein -
  WM2015_RS09615 (WM2015_1896) gspE 2266747..2268225 (+) 1479 WP_049725839.1 type II secretion system ATPase GspE -
  WM2015_RS09620 (WM2015_1897) - 2268230..2269426 (+) 1197 WP_049725840.1 type II secretion system F family protein -

Sequence


Protein


Download         Length: 149 a.a.        Molecular weight: 15258.40 Da        Isoelectric Point: 7.0787

>NTDB_id=151966 WM2015_RS16360 WP_281173112.1 2251955..2252404(+) (pilA2) [Wenzhouxiangella marina strain KCTC 42284]
MNLPRNMQKQQGFTLIELMIVIAILAILLAIAIPAYQNYSIRAANSECVNLAASIKLAVSETAQSNGVLATGVATATAAG
VVPANVATPRCGSVTVAAGVITIASTGSDGTSAGTFTFTPTQSTINDSIQWNCTANHGNPQHVPAECRS

Nucleotide


Download         Length: 450 bp        

>NTDB_id=151966 WM2015_RS16360 WP_281173112.1 2251955..2252404(+) (pilA2) [Wenzhouxiangella marina strain KCTC 42284]
ATGAACCTTCCCCGCAATATGCAGAAGCAGCAGGGCTTCACCCTGATCGAACTCATGATCGTGATTGCCATTCTGGCCAT
CCTGCTGGCCATCGCAATCCCGGCTTACCAGAACTATTCGATCCGCGCCGCCAATAGTGAGTGCGTCAATCTGGCCGCCT
CCATCAAGCTGGCCGTATCCGAAACCGCCCAGTCGAACGGCGTACTGGCGACCGGTGTCGCGACTGCGACGGCTGCAGGC
GTTGTCCCGGCCAACGTTGCAACTCCCCGCTGCGGCAGTGTGACCGTCGCCGCAGGCGTGATCACGATCGCCAGCACGGG
CAGCGACGGCACGTCGGCAGGCACCTTCACCTTCACCCCGACGCAGTCCACCATCAACGACTCCATCCAGTGGAACTGCA
CGGCGAACCACGGGAACCCGCAGCACGTCCCGGCTGAGTGTCGTTCATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0K0XX46

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

51.408

95.302

0.49

  pilA2 Legionella pneumophila str. Paris

51.408

95.302

0.49

  pilA Ralstonia pseudosolanacearum GMI1000

37.278

100

0.423

  comP Acinetobacter baylyi ADP1

40.789

100

0.416

  pilE Neisseria gonorrhoeae strain FA1090

36.478

100

0.389

  pilE Neisseria gonorrhoeae MS11

35.625

100

0.383

  pilA/pilA1 Eikenella corrodens VA1

36.667

100

0.369

  pilA Pseudomonas aeruginosa PAK

34.839

100

0.362


Multiple sequence alignment