Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WM2015_RS09565 Genome accession   NZ_CP012154
Coordinates   2253206..2254930 (+) Length   574 a.a.
NCBI ID   WP_049725830.1    Uniprot ID   A0A0K0XX96
Organism   Wenzhouxiangella marina strain KCTC 42284     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2244465..2269677 2253206..2254930 within 0


Gene organization within MGE regions


Location: 2244465..2269677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WM2015_RS09530 (WM2015_1879) - 2244465..2246438 (-) 1974 WP_156201043.1 hypothetical protein -
  WM2015_RS09535 (WM2015_1880) - 2246707..2247600 (+) 894 WP_082169626.1 glycosyltransferase family 2 protein -
  WM2015_RS09540 - 2247659..2248663 (-) 1005 WP_156201046.1 hypothetical protein -
  WM2015_RS09545 (WM2015_1882) - 2248615..2249241 (-) 627 WP_049725826.1 class I SAM-dependent methyltransferase -
  WM2015_RS09550 (WM2015_1883) - 2249771..2251681 (+) 1911 WP_049725827.1 hypothetical protein -
  WM2015_RS16360 (WM2015_1884) pilA2 2251955..2252404 (+) 450 WP_281173112.1 pilin Machinery gene
  WM2015_RS16365 (WM2015_1885) - 2252660..2253097 (+) 438 WP_049725829.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  WM2015_RS09565 (WM2015_1886) pilB 2253206..2254930 (+) 1725 WP_049725830.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WM2015_RS09570 (WM2015_1887) pilC 2255170..2256390 (+) 1221 WP_049725831.1 type II secretion system F family protein Machinery gene
  WM2015_RS09575 (WM2015_1888) pilD 2256521..2257342 (+) 822 WP_049727044.1 A24 family peptidase Machinery gene
  WM2015_RS09580 (WM2015_1889) - 2257610..2258311 (-) 702 WP_049725832.1 response regulator transcription factor -
  WM2015_RS09585 (WM2015_1890) - 2258494..2260386 (-) 1893 WP_169751138.1 sulfatase-like hydrolase/transferase -
  WM2015_RS09590 (WM2015_1891) rho 2260519..2261775 (-) 1257 WP_049725834.1 transcription termination factor Rho -
  WM2015_RS09595 (WM2015_1892) trxA 2262042..2262368 (-) 327 WP_049725835.1 thioredoxin TrxA -
  WM2015_RS09600 (WM2015_1893) - 2262584..2263879 (+) 1296 WP_049725836.1 DEAD/DEAH box helicase -
  WM2015_RS09605 (WM2015_1894) - 2263948..2264844 (+) 897 WP_049725837.1 type II secretion system protein N -
  WM2015_RS09610 (WM2015_1895) - 2264841..2266757 (+) 1917 WP_049725838.1 secretin N-terminal domain-containing protein -
  WM2015_RS09615 (WM2015_1896) gspE 2266747..2268225 (+) 1479 WP_049725839.1 type II secretion system ATPase GspE -
  WM2015_RS09620 (WM2015_1897) - 2268230..2269426 (+) 1197 WP_049725840.1 type II secretion system F family protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62842.87 Da        Isoelectric Point: 5.2664

>NTDB_id=151957 WM2015_RS09565 WP_049725830.1 2253206..2254930(+) (pilB) [Wenzhouxiangella marina strain KCTC 42284]
MYAQSTPSLRLSNLGRKLVENGLMSEEQAIEAMAKAAEKGRTFTSHLVREKLIDAVGFAHVAAEDFGMPLIDLAAVDLKH
APTDIVQEDLLRKHLILPLTKRGKKLYVAAADPGNKAGMDEIAFSTGYSVETILAPFNAIDGAIDRALAGSAQAFSELTE
EEDELENLNFNTSDEEDEKSGNDASGADDAPVVRFVNKILVDAIRRGASDIHFEPYEEEYRVRFRIDGVLATQVKPPKRM
APRLAARIKVMSSLDIAERRIPQDGRIKLNLSKGQAFDFRVSTCPTLWGEKIVLRILDSSGAFLGPEKLGFEEEQKDAYL
AAINKPYGMVLVTGPTGSGKTVSLYTALGLLNGEGTNISTVEDPVEIRMMGVNQVQQNTKQGLTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHLVLSTLHTNDAPQSIARLANMGVPTYNIASTVHLVLAQRLGRTLHKCKKLEDVPDDAL
RKIGFKEDEIEEGIKIYQANGCDQCNQGYKGRTGVFQVMPISEAMEEIILKGGNALELGRQAEAEGIIDLRRAALNKVKR
GMLSLIEMNRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=151957 WM2015_RS09565 WP_049725830.1 2253206..2254930(+) (pilB) [Wenzhouxiangella marina strain KCTC 42284]
ATGTACGCCCAGTCCACGCCCTCGCTCAGACTCTCGAACCTCGGACGCAAGCTGGTCGAGAATGGCCTGATGTCGGAGGA
ACAGGCCATCGAGGCAATGGCCAAGGCCGCAGAAAAAGGGCGCACTTTCACCAGCCATCTGGTACGGGAAAAACTGATCG
ATGCCGTTGGCTTCGCGCACGTGGCCGCAGAGGATTTCGGCATGCCGCTTATCGACCTGGCGGCCGTCGACCTGAAGCAC
GCTCCGACAGACATCGTGCAGGAGGACCTGCTCCGCAAGCATCTGATTCTTCCCCTGACCAAGCGGGGCAAGAAACTCTA
CGTCGCAGCAGCCGATCCCGGCAACAAGGCCGGGATGGACGAGATCGCCTTCTCGACCGGCTACAGCGTTGAAACCATCC
TCGCCCCCTTCAACGCCATCGACGGCGCCATCGACAGGGCCCTGGCCGGCTCCGCGCAGGCGTTCAGCGAGCTGACCGAG
GAAGAAGACGAGCTCGAGAACCTCAACTTCAACACCAGCGACGAAGAGGACGAGAAGTCCGGCAATGACGCATCCGGCGC
CGATGATGCCCCCGTCGTTCGTTTCGTCAACAAGATTCTCGTCGACGCCATCCGTCGTGGCGCTTCGGACATTCATTTCG
AGCCCTACGAAGAAGAGTACCGAGTACGCTTCCGCATCGACGGCGTACTGGCGACCCAGGTCAAACCGCCAAAGCGCATG
GCACCGCGTCTGGCCGCGCGCATCAAGGTCATGTCCAGCCTGGATATCGCCGAGCGCCGCATCCCGCAGGATGGCCGCAT
CAAGCTGAACCTCTCCAAGGGTCAGGCCTTCGACTTCCGTGTTTCGACCTGCCCCACCCTGTGGGGCGAGAAGATCGTGC
TTCGAATCCTGGATTCCTCGGGCGCGTTCCTGGGCCCGGAGAAGCTGGGCTTCGAAGAAGAGCAGAAGGACGCCTACCTG
GCCGCCATCAACAAGCCCTACGGCATGGTGCTCGTCACGGGCCCGACCGGCTCCGGTAAGACGGTCTCCTTGTATACGGC
TCTCGGACTGCTCAATGGCGAGGGCACGAATATCTCCACCGTCGAGGACCCGGTCGAAATCCGCATGATGGGCGTCAATC
AGGTCCAGCAGAACACCAAGCAGGGTCTGACCTTCGCGGCAGCACTGCGGTCCTTCCTGCGCCAGGATCCCGATATCATC
ATGGTGGGTGAAATCCGTGACCTGGAAACTGCGGAGATCGCCATCAAGGCAGCCCAGACCGGTCACTTGGTGCTGTCCAC
CCTGCACACCAACGACGCCCCCCAGTCCATTGCTCGACTGGCGAATATGGGTGTGCCCACCTACAACATCGCTTCGACCG
TCCACCTGGTGCTGGCCCAGCGCCTCGGGCGAACTCTGCACAAGTGCAAGAAGCTGGAAGATGTCCCAGACGACGCCTTG
CGCAAGATCGGCTTCAAGGAAGACGAGATCGAAGAAGGCATCAAGATCTATCAGGCCAACGGCTGCGACCAGTGCAACCA
GGGCTACAAGGGACGAACCGGCGTTTTCCAGGTGATGCCCATTTCGGAAGCCATGGAGGAAATCATCCTCAAGGGCGGCA
ACGCACTGGAATTGGGGCGCCAGGCAGAGGCGGAGGGCATCATCGACTTGCGTCGGGCCGCACTCAACAAGGTCAAGCGG
GGGATGCTCAGCCTGATCGAGATGAACCGAGTGACCAAGGACTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(18-150)

Type II/IV secretion system protein (T2SSE)

(198-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K0XX96

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

51.13

100

0.512
  pilB Acinetobacter baylyi ADP1

50

100

0.502
  pilB Acinetobacter baumannii D1279779

49.912

99.129

0.495
  pilF Neisseria gonorrhoeae MS11

47.788

98.432

0.47
  pilB Vibrio cholerae strain A1552

47.703

98.606

0.47
  pilB Vibrio campbellii strain DS40M4

46.903

98.432

0.462
  pilB Vibrio parahaemolyticus RIMD 2210633

46.561

98.78

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

37.031

100

0.395
  pilF Thermus thermophilus HB27

37.653

99.477

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.128

99.477

0.369

Multiple sequence alignment