Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   WM2015_RS09570 Genome accession   NZ_CP012154
Coordinates   2255170..2256390 (+) Length   406 a.a.
NCBI ID   WP_049725831.1    Uniprot ID   -
Organism   Wenzhouxiangella marina strain KCTC 42284     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2244465..2269677 2255170..2256390 within 0


Gene organization within MGE regions


Location: 2244465..2269677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WM2015_RS09530 (WM2015_1879) - 2244465..2246438 (-) 1974 WP_156201043.1 hypothetical protein -
  WM2015_RS09535 (WM2015_1880) - 2246707..2247600 (+) 894 WP_082169626.1 glycosyltransferase family 2 protein -
  WM2015_RS09540 - 2247659..2248663 (-) 1005 WP_156201046.1 hypothetical protein -
  WM2015_RS09545 (WM2015_1882) - 2248615..2249241 (-) 627 WP_049725826.1 class I SAM-dependent methyltransferase -
  WM2015_RS09550 (WM2015_1883) - 2249771..2251681 (+) 1911 WP_049725827.1 hypothetical protein -
  WM2015_RS16360 (WM2015_1884) pilA2 2251955..2252404 (+) 450 WP_281173112.1 pilin Machinery gene
  WM2015_RS16365 (WM2015_1885) - 2252660..2253097 (+) 438 WP_049725829.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  WM2015_RS09565 (WM2015_1886) pilB 2253206..2254930 (+) 1725 WP_049725830.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WM2015_RS09570 (WM2015_1887) pilC 2255170..2256390 (+) 1221 WP_049725831.1 type II secretion system F family protein Machinery gene
  WM2015_RS09575 (WM2015_1888) pilD 2256521..2257342 (+) 822 WP_049727044.1 A24 family peptidase Machinery gene
  WM2015_RS09580 (WM2015_1889) - 2257610..2258311 (-) 702 WP_049725832.1 response regulator transcription factor -
  WM2015_RS09585 (WM2015_1890) - 2258494..2260386 (-) 1893 WP_169751138.1 sulfatase-like hydrolase/transferase -
  WM2015_RS09590 (WM2015_1891) rho 2260519..2261775 (-) 1257 WP_049725834.1 transcription termination factor Rho -
  WM2015_RS09595 (WM2015_1892) trxA 2262042..2262368 (-) 327 WP_049725835.1 thioredoxin TrxA -
  WM2015_RS09600 (WM2015_1893) - 2262584..2263879 (+) 1296 WP_049725836.1 DEAD/DEAH box helicase -
  WM2015_RS09605 (WM2015_1894) - 2263948..2264844 (+) 897 WP_049725837.1 type II secretion system protein N -
  WM2015_RS09610 (WM2015_1895) - 2264841..2266757 (+) 1917 WP_049725838.1 secretin N-terminal domain-containing protein -
  WM2015_RS09615 (WM2015_1896) gspE 2266747..2268225 (+) 1479 WP_049725839.1 type II secretion system ATPase GspE -
  WM2015_RS09620 (WM2015_1897) - 2268230..2269426 (+) 1197 WP_049725840.1 type II secretion system F family protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44216.87 Da        Isoelectric Point: 9.6468

>NTDB_id=151958 WM2015_RS09570 WP_049725831.1 2255170..2256390(+) (pilC) [Wenzhouxiangella marina strain KCTC 42284]
MATTKQELETFLWEGKDKRGKKLKGEKTGRNETIVKAELRQQGIMPSRVRKKPKALFGSTGKRISARDIAIFTRQLATML
TSGIPLVQSFTIISQAAENPRLKKLIKDIQNDVESGSTLAESVAKHPAYFDELYVNLVESGESAGVLDQVLDSIASYKER
IESIKGKVKKAMFYPATVMAVAISVTALLLIVVIPQFEQIFQSFGADLPAFTRLVIGASEFLQSNIFWILLVVGGGGYAV
VQLKKRNKPFAHFLDRMSLKIPVIGPVLEKSALARFASTLATTFAAGVPLVDALKTVAGATGNVVYEEAVLKIREDVSTG
HQMQLAMQQTGLFPPMLIQMTAIGEEAGSLDAMLNKVATFYEEEVNNTVDALSSLLEPLIIVFIGVVVGSLVVAMYLPIF
QMAAVM

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=151958 WM2015_RS09570 WP_049725831.1 2255170..2256390(+) (pilC) [Wenzhouxiangella marina strain KCTC 42284]
ATGGCGACGACCAAGCAGGAACTCGAGACTTTCCTCTGGGAAGGTAAGGACAAACGCGGCAAGAAGCTCAAGGGCGAGAA
GACGGGTCGAAACGAGACCATCGTCAAGGCGGAACTCCGCCAGCAAGGCATCATGCCGTCTCGGGTTCGCAAGAAGCCCA
AGGCTCTTTTCGGCTCGACCGGCAAGCGAATTTCCGCCAGGGACATCGCCATCTTCACCCGCCAGCTGGCCACCATGCTG
ACCTCGGGCATCCCTCTCGTCCAAAGCTTCACGATCATCTCCCAGGCGGCCGAAAATCCTCGACTGAAGAAACTGATCAA
GGACATCCAGAACGACGTCGAATCCGGCTCCACGCTGGCCGAGTCCGTCGCCAAACATCCGGCCTACTTCGACGAACTCT
ACGTCAATCTGGTGGAATCGGGCGAGAGCGCCGGTGTACTCGATCAGGTGCTGGATTCGATCGCGAGCTACAAGGAGCGG
ATCGAGTCGATCAAGGGCAAGGTCAAGAAGGCCATGTTCTACCCCGCGACCGTCATGGCGGTGGCCATCAGCGTCACGGC
CCTGCTGCTGATCGTCGTCATCCCGCAGTTCGAGCAGATCTTCCAGAGCTTCGGGGCCGATCTTCCGGCCTTCACTCGAC
TGGTGATCGGGGCGTCCGAGTTCCTGCAGAGCAATATCTTCTGGATTCTCCTGGTCGTCGGCGGCGGTGGTTATGCCGTC
GTGCAGCTCAAGAAGCGAAACAAGCCCTTCGCGCACTTCCTCGATCGAATGTCCCTGAAGATTCCCGTCATCGGCCCGGT
ACTGGAGAAGTCTGCTCTGGCCCGCTTCGCCAGCACACTGGCCACCACCTTTGCGGCGGGTGTACCGCTGGTCGATGCTC
TCAAGACCGTCGCCGGGGCCACCGGCAATGTGGTTTACGAAGAGGCCGTGCTGAAGATCCGAGAAGATGTTTCGACCGGC
CATCAGATGCAGTTGGCCATGCAGCAGACCGGCCTGTTTCCGCCAATGCTCATCCAGATGACCGCCATCGGTGAGGAAGC
CGGCTCACTGGACGCCATGTTGAACAAGGTGGCTACGTTCTACGAGGAAGAAGTCAACAATACGGTCGACGCACTGTCGA
GCCTGCTCGAACCTCTGATCATCGTGTTCATCGGCGTCGTGGTCGGCAGCCTCGTCGTCGCCATGTATCTTCCGATCTTC
CAGATGGCTGCGGTGATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

57.683

97.783

0.564
  pilC Pseudomonas stutzeri DSM 10701

55.528

98.03

0.544
  pilC Acinetobacter baumannii D1279779

51.597

100

0.517
  pilC Acinetobacter baylyi ADP1

51.724

100

0.517
  pilC Vibrio cholerae strain A1552

42.963

99.754

0.429
  pilG Neisseria meningitidis 44/76-A

42.677

97.537

0.416
  pilG Neisseria gonorrhoeae MS11

42.677

97.537

0.416
  pilC Vibrio campbellii strain DS40M4

40.876

100

0.414
  pilC Thermus thermophilus HB27

36.75

98.522

0.362

Multiple sequence alignment